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|本期目录/Table of Contents|

成年糖尿病大鼠心室肌细胞的分离

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第2期
页码:
209-210/214
栏目:
基础研究
出版日期:
2009-03-30

文章信息/Info

Title:
Isolation of ventricular myocytes of adult diabetes rats
作者:
邵阳贞; 张代民; 李勋; 蒋文平
苏州大学附属第一医院心内科，江苏 苏州 215006
Author(s):
SHAO Yang-zhen;  ZHANG Dai-min;  LI Xun;  JIANG Wen-ping
Department of Cardiology, First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu, China
关键词:
糖尿病; 心室肌细胞; 大鼠
Keywords:
diabetes mellitus;  ventricular myocytes;  rats
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的 探索成年糖尿病大鼠心室肌细胞成功分离的方法。方法 取体质量150～200 g的Sprague-Dawley大鼠20只，单次腹腔注射链脲佐菌素(STZ)建立DM大鼠模型，3周后取大鼠心脏行主动脉逆行灌流分离心室肌细胞；并在倒置显微镜下观察分离的单个心室肌细胞状态。结果 建模成功率达90%，糖尿病大鼠的血糖水平稳定维持在20 mmol/L以上；分离的单个心室肌细胞存活率达70%～90%，且纹理清晰状态好。结论 该糖尿病心室肌细胞的分离方法简单有效。
Abstract:
AIM To explore an effective method for successful isolation of ventricular myocytes of adult diabetic rats. METHODS The diabetic rat model was established by a single injection of streptozocin (STZ) peritoneally in Sprague-Dawley rats weighing 150-200 g. Ventricular myocytes were isolated by perfusion of enzymatic solution. The isolated ventricular myocytes were then observed under inverted microscope. RESULTS The successful establishment rate of diabetic rats was 90%, the blood glucose level stayed high above 20 mmol/L. The isolated ventricular myocytes were alive up to 70%-90% with good and clear stripes. CONCLUSION This simple method is an effective way for successful isolation of diabetic rat ventricular myocytes.

参考文献/References

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［3］ Shehadeh A, Regan TJ. Cardiac consequences of diabetes mellitus［J］. Clin Cardiol, 1995, 18(6):301-305.

［4］ Mordes JP, Rossini AA. Animal models of diabetes mellitus［M］// Marble A, Krall LP, Bradley RF, et al. Injoslin’s Diabetes Mellitus. 12th ed, Philadelphia: Christlieb AR,1985:110-137.

［5］ Celentano A, Vaccaro O, Tammaro P, et al. Early abnormalities of cardiac function in non-insulin dependent diabetes mellitus and impaired glucose tolerance［J］. Am J Cardiol, 1995, 76(16):1173-1176.

［6］ Fang ZY, Prins JB, Marwick TH. et al. Diabetic cardiomyopathy: evidence, mechanisms, and therapeutic implications［J］. Endocrine Society, 2004, 25(4):543-567.

［7］ Shimoni Y, Firek L, Severson D, et al. Short-term diabetes alters K+ currents in rat ventricular myocytes［J］. Circ Res, 1994, 74(4):620-628.

［8］ T Kono. Roles of collagenases and other proteolytic enzymes in the dispersal of animal tissues［J］. Biochim Biophys Acta, 1969, 178(2):397-400.

备注/Memo

备注/Memo:
收稿日期：2007-8-27.通讯作者: 李勋,教授,主要从事心律失常的研究Email:xunli58@hotmail.com 作者简介: 邵阳贞，住院医师，硕士Email:lenelotj@163.com
更新日期/Last Update: 2009-04-16