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|本期目录/Table of Contents|

早期心肌缺血缺氧性坏死中周细胞数量的变化

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第2期
页码:
211
栏目:
基础研究
出版日期:
2009-03-30

文章信息/Info

Title:
Number changes of pericytes in early myocardial ischemia necrosis
作者:
李永梅1; 3; 任立群1; 王心蕊2
吉林大学： 1.再生医学科学研究所病理室,2.基础医学院病理科，吉林 长春 130021；3.天津医科大学内分泌研究所病理室，天津 300070
Author(s):
LI Yong-mei1; 3;  REN Li-qun1;  WANG Xin-rui2
1.Department of Pathology, Institute of Frontier Medical Sciences, 2.Department of Pathology, Foundation Medical College, Jilin University, Changchun 130021, Jinlin, China; 3.Department of Pathology, Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China
关键词:
周细胞; 心肌纤维化; 免疫荧光染色; 激光共聚焦显微镜
Keywords:
pericyte;  myocardial fibrosis;  immunofluorescence staining;  laser scanning confocal microscopy
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的 探讨周细胞在心肌纤维化发生发展中的作用。方法 采用免疫荧光双重标记技术，将周细胞染色后，在激光扫描共聚焦显微镜下观察周细胞在早期心肌缺血缺氧性坏死中该细胞的数量变化情况。结果 激光共聚焦显微镜观察发现，内皮细胞定位明确的周细胞的数量在注射盐酸异丙肾上腺素（Isp） 4 h后明显增多，12 h后开始下降，48 h后显著低于正常水平（P<0.05）。结论 周细胞作为心肌中的间质细胞可能是心肌纤维化发生发展中的主要效应细胞。
Abstract:
AIM To exlpore the role of pericytes during the generation and the development of myocardial fibrosis. METHODS After pericytes were double-labeled with immunofluorescence, the changes of the number of pericytes in the early myocardial ischemia necrosis was observed under laser scanning confocal microscopy. RESULTS Observation under laser scanning confocal microscope demonstrated the number of pericytes around endothelial cells increased significantly 4 hours after injection of Isp, descended 12 hours after the injection, and the number of pericytes was markedly lower than the normal level 48 hours after the injection (P<0.05). CONCLUSION Pericytes as myocardial interstitial cells may be the main effector cells in the generation and development of myocardial fibrosis.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2007-5-25.通讯作者：任立群，教授，主要从事心血管分子病理的研究Email: renlq@jlu.edu.cn 作者简介：李永梅，助教，硕士Email:Liyongmei0811@sina.com
更新日期/Last Update: 2009-04-16