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|本期目录/Table of Contents|

高温应激对大鼠心肌血管紧张素Ⅱ的影响及其与心肌p22phox表达的关系

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第3期
页码:
304-308
栏目:
基础研究
出版日期:
2009-05-15

文章信息/Info

Title:
Effect of heat stress on angiotensinⅡ expression in rat myocardium and its relationship to p22phox
作者:
侯小玲; 赵连友; 薛玉生; 艾永飞; 景晓娟; 牛晓琳; 赵晓燕; 尚福军
第四军医大学唐都医院心血管内科，陕西 西安 710038
Author(s):
HOU Xiao-Ling;  ZHAO Lian-You;  XUE Yu-Sheng;  AI Yong-Fei;  JING Xiao-Juan;  NIU Xiao-Lin;  ZHAO Xiao-Yan;  SHANG Fu-Jun
Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi, China
关键词:
高温应激; 血管紧张素Ⅱ; 活性氧; p22phox
Keywords:
heat stress;  angiotensinⅡ;  reactive oxygen species;  p22phox
分类号:
R972.5
DOI:
-
文献标识码:
A
摘要:
目的 观察高温应激对大鼠心肌血管紧张素Ⅱ（AngⅡ)的影响,探讨心肌AngⅡ与心肌p22phox表达的关系。方法 将24只成年雄性SD大鼠，随机分为：对照组、高温组、高温高湿组，每组8只。喂养4周后，颈动脉插管测定平均动脉压。用放射免疫法测定血浆和心肌AngⅡ浓度。用比色法测定心肌活性氧(ROS)水平。应用逆转录聚合酶链式反应（RT-PCR）和免疫组化法检测心肌p22phox mRNA及蛋白的表达水平。结果 高温组和高温高湿组大鼠平均动脉压、AngⅡ浓度、ROS水平、p22phox mRNA及蛋白的表达水平与对照组比较，均有显著升高（P<0.01）;高温高湿组与高温组比较，无显著性差异。结论 高温应激使大鼠血压升高，这可能与AngⅡ水平上调有关。AngⅡ介导ROS生成增加，其机制可能是高温应激引起p22phox超常表达所致，提示高温应激可引起心脏损害。
Abstract:
AIM To study the effect of heat stress on the expression of angiotensinⅡ（AngⅡ） in the myocardium and to investigate the interrelation between AngⅡ and p22phox in the myocardium. METHODS Twenty-four SD rats were divided randomly into three groups: the control group, the high temperature and high humidity stress group, and the heat stress group. All examinations were performed four weeks later. Mean arterial blood pressure (MBP) was measured by carotid artery intubation and reactive oxygen species (ROS) was determined with spectrophotometer. The concentration of AngⅡ in the plasma and myocardium was detected respectively by radioimmunoassay and expression level of p22phox mRNA in myocardium was determined by reverse transcription polymerase chain reaction (RT-PCR). P22phox protein was detected by immunohistochemistry and semi-quantitative analysis in different groups. RESULTS Four weeks after heat stress, MBP in the heat stress group and the high temperature and high humidity stress group increased significantly (P<0.01), but no significant difference was observed in LVW/BW index (P>0.05) compared with those in the control group. The concentration of AngⅡ in the plasma and the myocardium and the ROS level of myocardium significantly increased (both P<0.01), and the expression of p22phox mRNA and protein levels of myocardium also increased significantly (P<0.01） in the heat stress group and the high temperature and high humidity stress group. CONCLUSION The concentration of AngⅡ in the plasma and myocardium and p22phox in the myocardium increase in heat stress conditions. Heat stress may be associated with over production of ROS, which is induced by increased expression of AngⅡ.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2009-3-2.基金项目：军队“十一五”重要军事医学基础研究项目资助（062074） 通讯作者：赵连友，教授，主任医师，主要从事高血压发病机制及相关疾病防治研究Email：zhaolyfmmu@yahoo.com.cn 作者简介：侯小玲，医师，硕士Email：houxiaoling.good@163.com
更新日期/Last Update: 2009-05-18