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|本期目录/Table of Contents|

益气活血复方芪丹通脉片对缺血/再灌注大鼠心肌中NF-κB表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第3期
页码:
325-328
栏目:
基础研究
出版日期:
2009-05-15

文章信息/Info

Title:
Effect of qidantongmai tablet on NF-κB expression in myocardium of rats with ischemia reperfusion injury
作者:
解娟1; 王宗仁1; 王跃民2; 李军昌1; 黄烨1
第四军医大学: 1.西京医院中医科，2.基础部生理学教研室，陕西 西安 710032
Author(s):
XIE Juan1;  WANG Zong-ren1;  WANG Yue-min2;  LI Jun-chang1;  HUANG Ye1
1.Department of Traditional Chinese Medicine, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
心肌缺血/再灌注; NF-κB表达; 芪丹通脉片; SD大鼠
Keywords:
myocardial ischemia/reperfusion;  NF-κB expression;  QDTMT;  SD rat
分类号:
R289.5
DOI:
-
文献标识码:
A
摘要:
目的 探讨益气活血复方芪丹通脉片（QDTMT）对缺血/再灌注(I/R)大鼠心肌中核因子-κB（NF-κB）表达的影响。方法 将SD大鼠48只随机分为6组(n=8),即假手术组、模型组、QDTMT低中高剂量组（0.36、1.08、3.24 g/kg）及恬尔心组（5 mg/kg）。各组均用生理盐水配制的等体积药液灌胃7 d,每日1次。采用冠脉结扎的方法，造成大鼠心肌I/R模型后, 采用Western blot和免疫组化染色法，检测各组大鼠心肌中NF-κB蛋白的表达。结果 与假手术组比较,模型组NF-κB的表达显著增多(P<0.01)。与模型组比较，QDTMT各剂量组和恬尔心组NF-κB的表达显著减少(P<0.01)。结论 QDTMT能抑制I/R大鼠心肌中NF-κB蛋白的表达，从而对心肌具有保护作用。
Abstract:
AIM To investigate the effect of qidantongmai tablet（QDTMT）on NF-κB in the rat model of ischemia reperfusion-mediated myocardial injury (RMMI). METHODS Forty-eight male SD rats were randomly divided into six experimental groups: sham operation group, control group, low dose QDTMT（0.36 g/kg） group, medium dose QDTMT（1.08 g/kg） group, high dose QDTMT（3.24 g/kg） group and diltiazem hydrochloride（5 mg/kg） group. Different dosage of drug or saline at the same volume was administered to the animals once a day for seven days before operation. The model was established by ligating the coronary artery. Tissues in the reperfused region were sampled immediately and the protein levels of NF-κB were determined by Western bolt and immunohistochemical staining. RESULTS Compared with that in the sham operation group, the expression of NF-κB in the control group was obvious and the level of total NF-κB increased. Compared with that of control group, the expression of NF-κB was not obvious and the level of total NF-κB decreased in different QDTMT dosage groups as well as the diltiazem hydrochloride group. CONCLUSION QDTMT has some cardioprotective effect against RMMI in rats possibly by inhibiting the expression of NF-κB.

参考文献/References

[1］ Li C, Brorwder W, Kao RL. Early activation of transcription factor NF-κB during ischemia in perfused rat heart ［J］. Am J Physiol, 1999, 276(2 Pt 2):H543-H552.

［2］ 王宗仁，李军昌，王文，等. 芪丹通脉片对缺血再灌注大鼠心肌损伤的保护作用［J］. 中西医结合心脑血管病杂志， 2007， 5（9）：821-823.

［3］ 朱妙章,袁文俊,吴博威,等. 心血管生理学与临床［M］. 北京: 高等教育出版社, 2004:297-301.

［4］ Altavilla D, Deodato B, Campo GM, et al. IRFI 042， a novel dual vitamin E-like antioxidant，inhibits activation of nuclear factor-kappa B and reduces the inflammatory response in myocardial ischemia-reperfusion injury［J］. Cardiovasc Res, 2000, 47(3)：515-528.

［5］ Xuan YT, Tang XL, Banerjee S, et al. Nuclear factor κB play an essential role in the phase of ischemic preconditioning in conscious rabbits［J］. Circ Res, 1999, 84(9):1095-1109.

［6］ Verma IM, Stevenson JK, Svhwarz EM, et al. Rel/NF-kappa B/Ikappa B family: intimate tales of association and dissociation. ［J］. Gene Dev, 1995, 9(22):2723-2735.

［7］ Hiasa G, Hamada M, Ikeda S, et al. Ischemic preconditioning and lipopolysacharide attenuate nuclear factor-κB activation and gene expression of inflammatory cytokines in the ischemia-reperfusioned rat heart［J］. Jpn Circ J, 2001, 65(11):984-990.

［8］ Ke JJ, Wang YL, Li JG, et al. Pretreatment effect of adenosine on activation of NF-κB and level of TNF-α during myocardial ischemia and reperfusion in rats［J］. Chin J Traumatol, 2004, 7(1):25-27.

［9］ 李军昌，王宗仁，李秋霞，等. PI3K/Akt信号通路介导芪丹通脉片抑制缺血再灌注大鼠心肌细胞凋亡［J］. 中国现代医学杂志， 2007， 17（10）：1183-1186.

［10］黎沛环，王宗仁，李军昌，等. 芪丹通脉片对大鼠心肌缺血再灌注损伤时促炎细胞因子的影响［J］. 陕西医学杂志， 2007， 36（6）：645-646,659.

［11］王彬，王宗仁，李军昌，等. 芪丹通脉片对大鼠缺血/再灌注损伤心肌细胞GLUT1的影响［J］. 安徽中医学院学报， 2007， 26(2):29-32.

［12］王彬，王宗仁，李军昌，等. 芪丹通脉片对大鼠缺血/再灌注损伤心肌细胞GLUT4的影响［J］. 心脏杂志， 2007， 19（3）：255-257.

备注/Memo

备注/Memo:
收稿日期：2007-11-5.基金项目：“十一·五”军队中医药重大临床攻关专项课题基金资助(2006191001) 通讯作者：王宗仁，主任医师，主要从事中药防治心脑血管病的研究Email:zongren@fmmu.edu.cn 作者简介：解娟，硕士生Email:xjalice@126.com
更新日期/Last Update: 2009-05-18