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|本期目录/Table of Contents|

缺血预处理对兔心肌中肌钙蛋白T表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第3期
页码:
329-331
栏目:
基础研究
出版日期:
2009-05-15

文章信息/Info

Title:
Immunohistochemical study on influence of depletion of cardiac Troponin T in rabbits of myocardial infarction after ischemic preconditioning
作者:
刘娴1; 王晓云1; 朱红2; 王立峰1; 宁波1
1.哈尔滨医科大学附属第四医院心内科， 黑龙江 哈尔滨 150000; 2.首都医科大学宣武医院综合科， 北京 100053
Author(s):
LIU Xian1;  WANG Xiao-yun1;  ZHU Hong2;  WANG Li-feng1;  Ning Bo1
1.Department of Cardiology, Fourth Affiliated Hospital of Haebin Medical University, Haebin 150000, Heilongjiang, China; 2.General Department, Xuanwu Medical University, Beijing 100053, China
关键词:
缺血预处理; 心肌梗死; 肌钙蛋白T; 兔
Keywords:
ischemic preconditioning;  myocardial infarction;  troponin T;  Rabbit
分类号:
Q593.3
DOI:
-
文献标识码:
A
摘要:
目的 探讨心肌缺血预处理（IP）对心肌中肌钙蛋白T（cTnT）表达的影响。方法 将45只兔随机分为3组，即假手术组（只开胸、穿线不结扎冠脉）、直接梗死组（开胸、并结扎冠脉）及IP组（结扎冠脉5 min，再灌注10 min，反复3次）。于术后15 min、30 min、1 h、3 h及5 h处死动物，切取心肌，用免疫组化染色法检测心肌中cTnT的表达，并进行计算机图像分析。在高倍光镜下，测量心肌中不表达cTnT（cTnT-）的面积，取其平均值进行统计学分析。结果 比较同一时段3个组cTnT-的面积，发现IP组cTnT-的面积大于假手术组,但小于直接梗死组（P<0.05）。结论 IP明显减少缺血心肌的cTnT面积。
Abstract:
AIM To prove whether ischemic preconditioning is protective against myocardial ischemia. METHODS Myocardial tissues were obtained from models of three groups in different periods and immunohistochemical method was used to detect the expression of cTnT. The depletion areas of cTnT in myocardial infarction were measured under the microscope by the computer image analysis and the results were analyzed by statistical methods. RESULTS The depletion areas of the control group, the preconditioning group and the infarction group increased gradually in the same period and the result was significant. CONCLUSION Ischemic preconditioning is protective against myocardial ischemia.

参考文献/References

[1］ Murry CE, Jennings RB, Reimer KA, et al. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium［J］. Circulation, 1986, 74(5):1124-1136.

［2］ Marber MS, Latchman DS, Walker JM, et al. Cardiac stress protein elevation 24 hours after brief ischemia or heat stress is associated with resistance to myocardial infarction［J］. Circulation, 1993, 88(3):1264-1272.

［3］ 祝宝华,奚群英,刘晨,等. 细胞膜钠钙交换蛋白在大鼠心肌缺血预适应及腺苷诱导预适应中的作用及其信号转导［J］. 中华心血管病杂志, 2006, 34(4):367-371.

［4］ Fukuzwa S, Ozawas S, Inagaki M, et al. Nicorandil affords cardioprotection in patients with acute myocardial infarction treated with primary percutaneous transluminal coronary angioplasty: assessment with thallium-201/iodine-123 BMIPP dual SPECT［J］. J Nucl Cardiol, 2000, 7(5):447-453.

［5］ Kawakami T, Lwbeer C, Valen G, et al. Post-ischaemic dysfunction does not correlate with release of cardiac troponin T in isolated rat hearts［J］. Acta Physiol Scand, 1999, 167(1):23-27.

［6］ 姚尖平,张希,吴中凯,等. 缺血预处理在心瓣膜置换术中对心肌保护的作用［J］. 中国胸心血管外科临床杂志, 2006, 13(5):311-314.

［7］ Wu ZK, Pehkonen E, Laurikka J, et al. Ischemic preconditioning protects right ventricular function in coronary artery bypass grafting patients experiencing angina within 48-72 hours［J］. J Cardiovasc Surg(Torino), 2002, 43(3):319-326.

［8］ Kloner RA, Rezkalla SH. Preconditioning, postconditioning and their application to clinical cardiology［J］. Cardiovasc Res, 2006, 70(2):297-307.

备注/Memo

备注/Memo:
收稿日期：2008-3-11.通讯作者：王晓云，主任医师，主要从事心肌预处理的研究Email：wxy@medmail.com.cn 作者简介：刘娴，住院医师，硕士生Email：liuxian1979@126.com
更新日期/Last Update: 2009-05-18