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益气活血复方芪丹通脉片对缺血/再灌注大鼠心肌中NF-κB表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第3期
页码:
325-328
栏目:
基础研究
出版日期:
2009-05-15

文章信息/Info

Title:
Effect of qidantongmai tablet on NF-κB expression in myocardium of rats with ischemia reperfusion injury
作者:
解娟1王宗仁1王跃民2李军昌1黄烨1
第四军医大学: 1.西京医院中医科,2.基础部生理学教研室,陕西 西安 710032
Author(s):
XIE Juan1 WANG Zong-ren1 WANG Yue-min2 LI Jun-chang1 HUANG Ye1
1.Department of Traditional Chinese Medicine, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
心肌缺血/再灌注NF-κB表达芪丹通脉片SD大鼠
Keywords:
myocardial ischemia/reperfusion NF-κB expression QDTMT SD rat
分类号:
R289.5
DOI:
-
文献标识码:
A
摘要:
目的 探讨益气活血复方芪丹通脉片(QDTMT)对缺血/再灌注(I/R)大鼠心肌中核因子-κB(NF-κB)表达的影响。方法 将SD大鼠48只随机分为6组(n=8),即假手术组、模型组、QDTMT低中高剂量组(0.36、1.08、3.24 g/kg)及恬尔心组(5 mg/kg)。各组均用生理盐水配制的等体积药液灌胃7 d,每日1次。采用冠脉结扎的方法,造成大鼠心肌I/R模型后, 采用Western blot和免疫组化染色法,检测各组大鼠心肌中NF-κB蛋白的表达。结果 与假手术组比较,模型组NF-κB的表达显著增多(P<0.01)。与模型组比较,QDTMT各剂量组和恬尔心组NF-κB的表达显著减少(P<0.01)。结论 QDTMT能抑制I/R大鼠心肌中NF-κB蛋白的表达,从而对心肌具有保护作用。
Abstract:
AIM To investigate the effect of qidantongmai tablet(QDTMT)on NF-κB in the rat model of ischemia reperfusion-mediated myocardial injury (RMMI). METHODS Forty-eight male SD rats were randomly divided into six experimental groups: sham operation group, control group, low dose QDTMT(0.36 g/kg) group, medium dose QDTMT(1.08 g/kg) group, high dose QDTMT(3.24 g/kg) group and diltiazem hydrochloride(5 mg/kg) group. Different dosage of drug or saline at the same volume was administered to the animals once a day for seven days before operation. The model was established by ligating the coronary artery. Tissues in the reperfused region were sampled immediately and the protein levels of NF-κB were determined by Western bolt and immunohistochemical staining. RESULTS Compared with that in the sham operation group, the expression of NF-κB in the control group was obvious and the level of total NF-κB increased. Compared with that of control group, the expression of NF-κB was not obvious and the level of total NF-κB decreased in different QDTMT dosage groups as well as the diltiazem hydrochloride group. CONCLUSION QDTMT has some cardioprotective effect against RMMI in rats possibly by inhibiting the expression of NF-κB.

参考文献/References

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[10]黎沛环,王宗仁,李军昌,等. 芪丹通脉片对大鼠心肌缺血再灌注损伤时促炎细胞因子的影响[J]. 陕西医学杂志, 2007, 36(6):645-646,659.

[11]王彬,王宗仁,李军昌,等. 芪丹通脉片对大鼠缺血/再灌注损伤心肌细胞GLUT1的影响[J]. 安徽中医学院学报, 2007, 26(2):29-32.

[12]王彬,王宗仁,李军昌,等. 芪丹通脉片对大鼠缺血/再灌注损伤心肌细胞GLUT4的影响[J]. 心脏杂志, 2007, 19(3):255-257.

备注/Memo

备注/Memo:
收稿日期:2007-11-5.基金项目:“十一·五”军队中医药重大临床攻关专项课题基金资助(2006191001) 通讯作者:王宗仁,主任医师,主要从事中药防治心脑血管病的研究Email:zongren@fmmu.edu.cn 作者简介:解娟,硕士生Email:xjalice@126.com
更新日期/Last Update: 2009-05-18