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地黄低聚糖对人脂肪组织源性间充质干细胞分泌血管内皮细胞生长因子的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第3期
页码:
332-335，346
栏目:
基础研究
出版日期:
2009-05-15

文章信息/Info

Title:
The effect of rehmannia glutinosa oligosaccharide on vascular endothelial cell growth factor secreted by human adipose tissue-derived mesenchymal stem cells
作者:
王玉红1; 2; 陈光辉1; 王舒1; 张琰琴1; 王磊1; 秦宇红3
1.解放军总医院心血管内科，北京 100853； 2.北京军区总医院急诊科， 北京 100700； 3.解放军总医院第一附属医院急诊科，北京 100037
Author(s):
WANG Yu-hong1; 2;  CHEN Guang-hui1;  WANG Shu1;  ZHANG Yan-qin1;  WANG Lei1 ; QIN Yu-hong3
1.Department of Cardiology,General Hospital of PLA, Beijing 100853, China; 2.Department of Emergency, General Hospital, Beijing Military Area Command, Beijing 100700, China； 3.Emergency and Burns Institute, First Affiliated Hospital, General Hospital of P
关键词:
脂肪组织源性间充质干细胞; 血管内皮生长因子; 地黄低聚糖
Keywords:
adipose tissue-derived mesenchymal stromal cells;  vascular endothelial cell growth factor;  rehmannia glutinosa oligosaccharide
分类号:
R282.71
DOI:
-
文献标识码:
A
摘要:
目的 探讨地黄低聚糖(RGO)对分离、培养的人脂肪组织源性间充质干细胞(ADMSCs)分泌血管内皮细胞生长因子(VEGF)的影响。方法 人脂肪组织用2.5 g/L胶原蛋白酶Ⅰ消化、分离ADMSCs。取沉淀的细胞进行培养，用免疫细胞化学染色法鉴定其表面CD44、CD105、CD45和CD34分子的表达。以IMDM培养基作为空白对照，加入RGO配成不同浓度（0、1、10、100及400 mg/L）的IMDM分别培养ADMSCs，用MTT比色法检测ADMSCs的增殖。培养72 h后，用ELISA法测定不同培养基中VEGF的含量。结果 免疫细胞化学染色显示，分离培养的细胞表面CD44+、CD105+、CD34-、CD45-。MTT比色法的结果显示，与空白对照组相比，1和10 mg/L组无明显差别，100及400mg/L组明显升高（均P<0.01），且100mg/L组明显高于400mg/L组(P<0.05)。0、1、10、100、400mg/L5个组VEGF的含量，分别为(627.88±33.86)、(655.50±40.29)、(666.50±43.20)、（843.50±53.05）和（722.88±51.34）ng/L。结论 RGO对ADMSCs 的增殖有促进作用，且呈一定的浓度依赖性，并导致其分泌VEGF的作用增强。
Abstract:
AIM To isolate and culture human adipose tissue-derived mesenchymal stromal cells (hADMSCs) and to study the effect of rehmannia glutinosa oligosaccharide（RGO） on the secretion of vascular endothelial cell growth factor(VEGF) from ADMSCs. METHODS Human adipose tissue from patients receiving abdominal surgery was digested with 2.5 g/L collagenase typeⅠ, hADMSCs were separated and cells from sediment were cultured. The expression of CD44, CD105, CD45 and CD34 on ADMSCs were examined by immunocytochemical staining. ADMSCs were cultured in IMDM culture medium containing different concentrations (0，1，10，100，400 mg/L) of RGO. The effects of different concentrations of RGO on hADMSCs proliferation were measured by MTT colorimetry. At 72 hours after culture, ELISA was used to detect the level of VEGF in different culture mediums. RESULTS ADMSCs were identified by positive expression of CD44 and CD105 and negative expression of CD34 or CD45. According to MTT colorimetry’s results, there was little and insignificant difference in proliferation between groups of 1 mg/L and 10 mg/L and control group. Significant proliferation was seen in ADMSCs of groups of 100 mg/L and 400 mg/L (P<0.01). The contents of VEGF were (627.88±33.86), (655.50±40.29), (666.50±43.20), （843.50±53.05） and （722.88±51.34）ng/L, respectively. CONCLUSION RGO has significant effect on ADMSCs’s proliferation and can promote secretion of VEGF.

参考文献/References

[1］ Gimble J, Guilak F. Adipose-derived adult stem cells: isolation, characterization, and differentiation potential［J］. Cytotherapy, 2003, 5(5):362-369.

［2］ Miranville A, Heeschen C, Sengenes C, et al. Improvement of postnatal neovascularization by human adipose tissue-derived stem cells［J］. Circulation, 2004, 110(3):349-355.

［3］ Rehman J, Traktuev D, Li J, et al. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells［J］ . Circulation, 2004, 109（10）:1292-1298.

［4］ 尹明. 地黄低聚糖对自体骨骼肌成肌细胞移植治疗心肌梗死效应及机制的研究［D］. 北京:解放军军医进修学院， 2006:35-36.

［5］ Risau W. Mechanisms of angiogenesis［J］. Nature, 1997, 386（6626）:671-674.

［6］ Henry TD. Therapeutic angiogenesis［J］. BMJ, 1999, 318(7197):1536-1539.

［7］ 霍勇,高炜,丁文惠. 心肌梗死［M］. 沈阳: 辽宁科学技术出版社, 2003:417.

［8］ Spyridopoulos I, Brogi E, Kearney M, et al. VEGF inhibits endothelial cell apoptosis induced by tumor necrosis factor-α: balance between growth and death signals［J］. J Mol Cell Cardiol, 1997, 29(5):1321-1330.

［9］ Losordo DW, Vale PR, Symes JF, et al. Gene therapy for myocardial angiogenesis, initial clinical results with direct myocardial injection of phVEGF 165 as sole therapy for myocardial ischemia［J］. Circulation, 1998, 98(25):2800-2804.

［10］叶定江,张世臣. 中药炮制学［M］. 北京: 人民卫生出版社, 1999:289-294.

［11］刘福军，程军平，赵修南，等. 地黄多聚糖对正常小鼠造血干细胞、祖细胞及外周血像的影响［J］. 中药药理与临床， 1996， 12（2）：14-16.

［12］De Ugarte DA, Alfonso Z, Zuk PA, et al. Differential expression of stem cell mobilization-associated molecules on multilineage cells from adipose tissue and bone marrow［J］. Immunol Lett, 2003, 89(2-3):267-270.

［13］吴志奎，王蕾，蔡永春，等. 糖生物学研究与传统中医药发展［J］. 深圳中西医结合杂志， 2001， 12(2):73-75.

备注/Memo

备注/Memo:
收稿日期：2007-12-14.基金项目：国家自然科学基金项目资助(30471924/30572445) 通讯作者：陈光辉，副主任医师，主要从事心衰发病机制及防治的研究Email:chenkevin301@msn.com 作者简介：王玉红，副主任医师，硕士生Email:redwinny@sina.com
更新日期/Last Update: 2009-05-18