染料介导的光氧化处理去细胞猪主动脉瓣的细胞毒性研究(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2009年第4期
- 页码:
- 496-499
- 栏目:
- 基础研究
- 出版日期:
- 2009-06-25
文章信息/Info
- Title:
- Cytotoxicity of acellular porcine heart valve treated with dye-mediated photooxidation
- Author(s):
- LIU Yang1; SUN Guo-cheng1; LIU Shan-shan2; ZHENG Qi-jun1; GU Chun-hu1; YI Ding-hua1; LIU Wei-yong1
- 1.Department of Cardiac Surgery, Xijing Hospital, Xian 710032, Shaanxi, China, 2.Department of Ultrasound Diagnosis, Tangdu Hospital, Fourth Military Medical University, Xian 710038, Shaanxi, China
- Keywords:
- dye-mediated photooxidation; acellular; bioprosthetic valve; tissue engineering; cytotoxicity assay
- 分类号:
- R318.11
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 评价染料亚甲基蓝介导的光氧化处理去细胞猪主动脉瓣膜的细胞毒性。方法 将L929细胞经亚甲基蓝介导光氧化处理去细胞猪主动脉瓣浸提液培养后，用MTT比色法测定其相对增殖率。将L929细胞与染料介导光氧化处理去细胞猪主动脉瓣直接接触培养，于不同时间观察细胞的生长状态。将L929细胞种植于染料介导的光氧化处理去细胞猪主动脉瓣表面，用扫描电镜观察其生长情况。结果 染料亚甲基蓝介导的光氧化处理去细胞猪主动脉瓣的性质稳定，细胞毒性程度为0～Ⅰ级。L929细胞与瓣膜材料直接接触培养生长良好，形态学无明显改变。结论 染料亚甲基蓝介导的光氧化处理去细胞猪主动脉瓣的细胞相容性良好。
- Abstract:
- AIM: To evaluate the cytotoxicity of acellular porcine heart valve treated with dye-mediated photooxidation. METHODS: L929 cells were cultured in the extraction obtained from acellular porcine heart valve with dye-mediated photooxidation to measure the relative growth rate by methyl-thiazolyl-tetrazolium (MTT) assay. L929 cells were cultured directly with acellular porcine heart valve with dye-mediated photooxidation. Cell morphology was observed and the cell number was counted. Morphology of L929 cells cultured on the surface of acellular porcine heart valve with dye-mediated photooxidation was examined by scanning electron microscopy. RESULTS: The property of acellular porcine heart valve with dye-mediated photooxidation was stable. Toxic levels ranged from grade 0 to I . There were no signs of cytotoxicity induced by acellular porcine heart valve with dye-mediated photooxidation, and cell morphology was normal. CONCLUSION: Acellular porcine heart valve with dye-mediated photooxidation shows good cytocompatibility in vitro.
参考文献/References
［1］ Jorge-Herrero E, Fernandez P, Turnay J, et al. Influence of different chemical crosslinking treatments on the properties of bovine pericardium and collagen［J］. Biomaterials, 1999, 20(6):539-545.
［2］ Nugent HM, Edelman ER. Tissue engineering therapy for cardiovascular disease［J］. Circ Res, 2003, 92(10):1068-1078.
［3］ Chang Y, Hsu CK, Wei HJ, et al. Cell-free xenogenic vascular grafts fixed with glutaraldehyde or genipin: In vitro and in vivo studies［J］. J Biotechnol, 2005, 120(2):207-219.
［4］ Moore MA, Adams AK. Calcification resistance, biostability and low immunogenic potential of porcine heart valves modified by dye-mediated photooxidation［J］. J Biomed Mater Res, 2001, 56(1):24-30.
［5］ Moore MA, Bohachevsky IK, Cheung DT, et al. Stabilization of pericardial tissue by dye-mediated photooxidation［J］. J Biomed Mater Res, 1994, 28(5):611-618.
［6］ Adams AK, Talman EA, Campbell L, et al. Crosslink formation in porcine valves stabilized by dye-mediated photooxidation［J］. J Biomed Mater Res, 2001, 57(4):582-587.
［7］ Svendsen CA, Kreykes NS, Butany J, et al. In vivo assessment of a photofixed bovine pericardial valve［J］. J Heart Valve Dis, 2000, 9(6):813-820.
［8］ Schmidt CE, Baier JM. Acellular vascular tissue: natural biomaterials for tissue engineering［J］. Biomaterials, 2000, 21(22):2215-2231.
［9］ Breymann T, Thies WR, Boehtig DR. et al. Bovine valved xenografts for RVOT reconstruction: results after 71 implantations［J］. Eur J Cardiothorac Surg, 2002, 21(4):703-710.
［10］Fann JI, Miller DC, Moore KA, et al. Twenty-year clinical experience with porcine bioprosthesis［J］. Ann Thorac Surg, 1996, 62(5):1301-1311.
备注/Memo
- 备注/Memo:
- 收稿日期：2008-3-24.基金项目：国家高技术研究发展计划（863计划）课题项目资助(2006AA02A138)；陕西省科技攻关课题项目资助［2007K11-03（1）］ 通讯作者：孙国成，教授，主任医师，主要从事婴幼儿先心病的外科治疗研究Email：sungch@fmmu.edu.cn 作者简介：刘洋，博士生Email: liuyang2@fmmu.edu.cn
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