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|本期目录/Table of Contents|

重组人松弛素体外调节血管紧张素Ⅱ诱导的大鼠心肌纤维化

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第1期
页码:
30-32
栏目:
基础研究
出版日期:
2010-01-04

文章信息/Info

Title:
Recombinant human relaxin modulates angiotensin II-induced cardiac fibrosis
作者:
王萍; 李虹伟; 王宇朋; 严松彪; 陈晖; 马文英; 贾三庆
首都医科大学附属北京友谊医院心脏中心，北京 100050
Author(s):
WANG Ping;  LI Hong-wei;  WANG Yu-peng;  YAN Song-biao;  CHEN Hui;  MA Wen-ying;  JIA San-qing
Cardiac Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
关键词:
重组人松弛素; 胶原; 血管紧张素Ⅱ
Keywords:
rhRLX;  collagen;  angiotensin II
分类号:
R542.23
DOI:
-
文献标识码:
A
摘要:
目的: 观察在重组人松弛素（rhRLX）及血管紧张素Ⅱ（AngⅡ）作用下对心脏成纤维细胞功能（CFs）的影响。方法:原代培养Sprague-Dawley大鼠心脏成纤维细胞，经AngⅡ（10-6mmol/L）单独作用及AngⅡ加rhRLX(100μg/L)共同作用后，用MTT比色法测定CFs的增殖；用ELISA法检测Ⅰ型前胶原羧基末端肽（PⅠCP）和Ⅲ型前胶原氨基末端肽（PⅢNP）的水平。结果: rhRLX可显著抑制AngⅡ促进CFs增殖，并拮抗AngⅡ促进CFs分泌PⅠCP的作用。结论: rhRLX能够改善AngⅡ诱导的心肌纤维化。
Abstract:
AIM: To observe the influence of recombinant human relaxin (rhRLX) on cardiac fibroblast (CF) functions under angiotensin II (AngII). METHODS: We cultured neonatal rat CFs in AngII (10-6 mmol/L) conditions in the absence or presence of rhRLX (100 μg/L). The cell growth of CF rate was determined by MTT colorimetry. Levels of procollagen type I C-terminal peptide (PICP) and procollagen type III amino terminal peptide (PIIINP) were detected by ELISA. RESULTS: rhRLX significantly inhibited the proliferation of cultured CF induced by AngII (P<0.01). AngII accelerated the production of PICP in CFs. rhRLX blocked collagen synthesis induced by AngII. CONCLUSION: rhRLX ameliorates cardiac fibrosis induced by AngII.

参考文献/References

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［3］ Samuel CS, Hewitson TD, Zhang Y, et al. Relaxin ameliorates fibrosis in experimental diabetic cardiomyopathy［J］. Endocrinology, 2008, 149(7):3286-3293.

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［7］ Dschietzig T, Bartsch C, Richter C, et al. Relaxin, a pregnancy hormone, is a functional endothelin-1 antagonist: attenuation of endothelin-1-mediated vasoconstriction by stimulation of endothelin type-B receptor expression via ERK-1/2 and nuclear factor-kappaB［J］. Circ Res, 2003, 92(1):32-40.

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备注/Memo

备注/Memo:
收稿日期：2008-9-16.通讯作者：李虹伟，主任医师，博士，主要从事糖代谢与冠心病的关系研究 Email:mcw19656@yahoo.com.cn 作者简介：王萍，主治医师，博士Email:wangping1@medmail.com.cn
更新日期/Last Update: 2010-01-05