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Title:: Effects of glucagon-like peptide-1 on injury of neonatal mice cardiomyocytes induced by hypoxia-reoxygenation

作者:: 周雪¹; 谢云²; 汪玮琳²; 钟成福¹; 沙伟伟²; 王绍欣²; 李贲³; 1.保定解放军第252医院内分泌科，3.保定第三医院胸外科，河北保定 071000；2.天津医科大学代谢病医院神经内科，天津 300070

Author(s):: ZHOU Xue¹; XIE Yun²; WANG Wei-lin²; ZHONG Cheng-fu¹; SHA Wei-wei²; WANG Shao-xin²; LI Ben³; 1.Department of Endocrinology, PLA 252 Hospital, Baoding 071000, Hebei, China; 2.Department of Neurology, Metabolic Disease Hospital, Tianjin Medical University, Tianjin 300070, China; 3.Department of Thoracic Surgery, Baoding Third Hospital, Baoding 071000, Hebei, China

关键词:: 胰高血糖素样肽-1; 心肌细胞; 凋亡; 半胱氨酸天冬氨酸特异性蛋白酶-3

Keywords:: GLP-1; cardiomyocytes; apoptosis; caspase-3

分类号:: R975

DOI:: -

文献标识码:: A

摘要:: 目的: 研究胰高血糖素样肽-1（glucagon-like peptide-1，GLP-1）对缺氧/复氧(H/R)诱导的乳鼠心肌细胞损伤的影响及其可能的机制。方法: 将体外培养乳鼠心肌细胞分为3组，即正常对照组、H/R组和GLP-1+H/R组。H/R损伤后，分别通过比色法、流式细胞术、荧光检测法测定上清液中乳酸脱氢酶（LDH）的活性、心肌细胞的凋亡率及半胱氨酸天冬氨酸特异性蛋白酶-3（caspase-3）的活性。结果: 与正常对照组比较，H/R组LDH的活性、心肌细胞的凋亡率及caspase-3的活性均明显增高(P<0.01)；而与H/R组相比，GLP-1+H/R组LDH的活性、心肌细胞的凋亡率及caspase-3的活性则明显降低(P<0.01)。结论: GLP-1可直接作用于心肌细胞，对H/R诱导的心肌细胞损伤产生一定的拮抗作用，其作用机制可能主要是通过抑制心肌细胞的凋亡所致；而GLP-1对心肌细胞凋亡的抑制作用，可能与其对caspase-3相关的凋亡或抗凋亡途径的调节有关。

Abstract:: AIM: To study the effects of glucagon-like peptide-1 (GLP-1) on the injury of neonatal mice cardiomyocytes induced by hypoxia-reoxygenation (H/R) and to explore the possible mechanism. METHODS: Cultured neonatal mice cardiomyocytes were randomly divided into three groups: normal control group, H/R group, and H/R and GLP-1 group. LDH activity, apoptosis rate of cardiomyocytes and caspase-3 activity were detected. RESULTS: Compared with the normal control group, LDH activity, cardiomyocyte apoptosis rate and caspase-3 activity all increased significantly in the H/R group. Compared with the H/R group, the above three indexes all decreased in the H/R and GLP-1 groups. CONCLUSION: GLP-1 directly acts on cardiomyocytes and protects them from H/R injury mainly by inhibiting apoptosis. GLP-1 may exert its apoptotic effects through regulation of apoptotic or antiapoptotic pathways of caspase-3.

参考文献/References

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胰高血糖素样肽-1对缺氧/复氧诱导的乳鼠心肌细胞损伤的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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