我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

NDRG2在大鼠心肌缺血再灌注损伤中的表达

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第4期
页码:
529-532
栏目:
基础研究
出版日期:
2010-06-10

文章信息/Info

Title:
NDRG2 expression of rat heart in ischemia/reperfusion injury
作者:
孙中婵1; 沈岚2; 孙冬冬1; 曹丰1; 药立波2; 王海昌1
第四军医大学：1.西京医院心内科，2.基础部生化教研室，陕西 西安 710032
Author(s):
SUN Zhong-chan1;  SHEN Lan2;  SUN Dong-dong1;  CAO Feng1;  YAO Li-bo2;  WANG Hai-chang1
1.Department of Cardiology, Xijing Hospital, 2.Department of Biochemistry and Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
NDRG2; 缺血再灌注损伤; 心肌; 大鼠
Keywords:
NDRG2;  ischemia/reperfusion injury;  myocardium;  rats
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的: 观察大鼠心肌缺血或心肌缺血再灌注损伤(RMMI)时，心肌组织中N-Mcy下游调节基因(NDRG2)表达的变化。方法: 将60只健康成年SD大鼠随机分为对照组、单纯缺血组和缺血/再灌注(I/R)组，每组5~6只。单纯缺血组：套扎冠脉，选取缺血3、6、12、24 h4个时间点。再灌注组分别选择再灌注后6、12、24和48 h后获取心脏。结果: 单纯缺血组各时间点NDRG2的表达与对照组相比无显著差异，NDRG2 mRNA和其蛋白表达的变化一致。再灌注组与对照组比较，随着时间的延长，NDRG2 mRNA的表达逐渐降低，在缺血12 h达到最低（P<0.01），NDRG2蛋白的表达在缺血24 h达到最低（P<0.01）。结论: 单纯心肌缺血不引起NDRG2基因及其蛋白表达水平的变化，但再灌注损伤可下调心肌细胞中NDRG2的表达。
Abstract:
AIM: To investigate alterations caused by ischemia alone or ischemia/reperfusion in cardiac gene expression of NDRG2. METHODS: Expressions of NDRG2 in rat hearts exposed to ischemia stress alone or to ischemia/reperfusion were observed at 3, 6, 12 and 24 h of ischemia and at 6, 12, 24 and 48 h of reperfusion preceded by 30 min of ischemia induced by coronary artery ligation. RESULTS: Results demonstrated that the NDRG2 mRNA expression in cardiac tissue significantly decreased after 12 h of reperfusion and NDRG2 protein expression significantly decreased after 24 h of reperfusion. However, ischemia alone exerted little influence on NDRG2 expression. CONCLUSION: Ischemia alone cannot cause alterations in NDRG2 gene expression but ischemia/reperfusion injury can downregulate NDRG2 gene expressions in myocardium.

参考文献/References

［1］Yao L, Zhang J, Liu X. NDRG2: a Myc-repressed gene involved in cancer and cell stress［J］. Acta Biochim Biophys Sin, 2008, 40(7):625-635.

［2］Wang L, Liu N, Yao L, et al. NDRG2 is a new HIF-1 target gene necessary for hypoxia-induced apoptosis in A549 cells［J］. Cell Physiol Biochem, 2008, 21(1-3):239-250.

［3］Hu XL, Liu XP, Lin SX, et al. NDRG2 expression and mutation in human liver and pancreatic cancers［J］. World J Gastroenterol, 2004, 10(23):3518-3521.

［4］Deng Y, Yao L, Chau L, et al. N-Myc downstream-regulated gene 2 (NDRG2) inhibits glioblastoma cell proliferation［J］. Int J Cancer, 2003, 106(3):342-347.

［5］Kim A, Kim MJ, Yang Y, et al. Suppression of NF-kappaB activity by NDRG2 expression attenuates the invasive potential of highly malignant tumor cells［J］. Carcinogenesis, 2009, 30(6):927-936.

［6］Foletta VC, Prior MJ, Stupka N, et al. NDRG2, a novel regulator of myoblast proliferation, is regulated by anabolic and catabolic factors［J］. J physiol, 2009, 587(Pt 7):1619-1634.

［7］Hu XL, Liu XP, Deng YC, et al. Expression analysis of the NDRG2 gene in mouse embryonic and adult tissues［J］. Cell and tissue research, 2006, 325(1):67-76.

［8］Fliss H, Gattinger D. Apoptosis in ischemic and reperfused rat myocardium［J］. Circulation research, 1996, 79(5):949-956.

[9]Ostadal P, Elmoselhi AB, Zdobnicka I, et al. Ischemia-reperfusion alters gene expression of Na+-K+ ATPase isoforms in rat heart［J］. Biochem Biophys Res Commun, 2003, 306(2):457-462.

备注/Memo

备注/Memo:
收稿日期：2009-08-20.通讯作者：药立波,教授,主要从事肿瘤生物学研究 Email:bioyao@fmmu.edu.cn 共同通讯作者：王海昌，主任医师，主要从事冠心病与心律失常诊疗研究Email:wanghc@fmmu.edu.cn 作者简介:孙中婵，硕士生Email:sunzhongchan@yahoo.cn
更新日期/Last Update: 2010-05-20