牙龈卟啉单胞菌对心血管内皮细胞黏附和入侵的研究
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2010年第5期
- 页码:
- 692-694
- 栏目:
- 基础研究
- 出版日期:
- 2010-06-22
文章信息/Info
- Title:
- Invasion of porphyromonas gingivalis to human coronary artery endothelial cells
- Author(s):
- SONG Hong; MA Qin; LV Xin; ZHAO Rui-ni
- Department of General Dentistry, School of Stomatoloy, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
- 分类号:
- R329.25
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的: 牙龈卟啉单胞菌(Porphyromonas gingivalis，P.g)作为牙周炎致病菌，同时也能引起心血管系统的疾病。在本研究中，比较4种P.g对人牙龈上皮细胞，人心血管内皮细胞，和人脐静脉内皮细胞的黏附和入侵。探讨P.g对人牙龈上皮细胞和心血管内皮细胞的黏附和入侵能力。方法: 通过抗菌素保护实验检测细菌对各种细胞的黏附和入侵能力。结果: 各种细菌的黏附和入侵能力是不同的，相同细菌对不同细胞的黏附和入侵也各有不同，P.g对人牙龈上皮细胞，人心血管内皮细胞的黏附和入侵明显高于对人脐静脉内皮细胞的黏附和入侵。结论: P.g菌株对牙龈上皮细胞和人心血管内皮细胞有着相似的黏附和入侵能力。
- Abstract:
- AIM: To explore the invasion of porphyromonas gingivalis (P.g), a periodontal pathogen, in the pathogenesis of coronary heart disease. METHODS: Four different strains of P.g were tested for adhesion and invasion of human gingival epithelial cells (HGEC), human umbilical vein endothelial cells (HUVEC) and human coronary artery endothelial cells (HCAEC). The strains were tested for the ability to attach to and invade HGEC, HUVEC and HCAEC using a standard antibiotic protection assay. RESULTS: Data indicated that the invasion efficiency of P.g was different among the different strains. The same strain showed a different ability to adhere to and invade different types of cells. The efficiency of P.g adhesion and invasion of HGEC and HCAEC was higher than HUVEC. CONCLUSION: P.g strains have a stronger ability to adhere to and invade both HGEC and HCAEC.
参考文献/References
［1］Chen T, Duncan MJ. Gingipain adhesin domains mediate Porphyromonas gingivalis adherence to epithelial cells［J］. Microb Pathog, 2004, 36(4):205-209.
［2］Dierickx K, Pauwels M, Laine ML, et al. Adhesion of Porphyromonas gingivalis serotypes to pocket epithelium［J］. J Periodontol, 2003, 74(6):844-848.
［3］Beck JD, Elter JR, Heiss G, et al. Relationship of periodontal disease to carotid artery intima-media wall thickness: the atherosclerosis risk in communities (ARIC) study［J］. Arterioscler Thromb Vasc Biol, 2001, 21(1):1816-1822.
［4］Socransky SS, Haffajee AD. The bacterial etiology of destructive periodontal disease:current concepts［J］. J Periodontol, 1992, 63(4 Suppl):322-331.
［5］ Spahr A, Klein E, Khuseyinova N, et al. Periodontal infections and coronary heart disease: role of periodontal bacteria and importance of total pathogen burden in the Coronary Event and Periodontal Disease (CORODONT) study［J］. Arch Intern Med, 2006, 166(5):554-559.
［6］Cavrini FV, Sambri A, Moter D, et al. Molecular detection of Treponema denticola and Porphyromonas gingivalis in coronary heart disease in the United States［J］. Infect Immun, 2005, 153(21):2489-2494.
［7］ Sambri V, Marangoni A, Cavrini F, et al. Need for procedural details in detection of periodontopathic bacterial DNA in the atheromatous plaque by PCR［J］. J Clin Microbiol, 2004, 42(10):4914-4915.
［8］ Nakano K, Inaba H, Nomura R, et al. Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients［J］. Oral Microbiol Immunol, 2008, 23(2):170-172.
[9]Janket SJ, Baird AE, Chuang SK, et al. Meta-analysis of periodontal disease and risk of coronary heart disease and stroke［J］. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2003, 95(5):559-569.
[10]Progulske-Fox A, Kozarov E, Dorn B, et al. Porphyromonas gingivalis virulence factors and invasion of cells of the cardiovascular system［J］.J Periodontal Res, 1999, 34(7):393-399.
[11]Amornchat C, Rassameemasmaung S, Sripairojthikoon W, et al. Invasion of Porphyromonas gingivalis into human gingival fibroblasts in vitro［J］. J Int Acad Periodontol, 2003, 5(4):98-105.
[12]Dorn BR, Harris LJ, Wujick CT, et al. Invasion of vascular cells in vitro by Porphyromonas endodontalis［J］.Int Endod J, 2002, 35(4):366-371.
备注/Memo
- 备注/Memo:
- 收稿日期：2009-08-13.作者简介:宋红,副主任医师Email:songhong@fmmu.edu.cn
常用功能
统计/Statistics
- 摘要浏览/Viewed1220
- 评论/Comments
