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|本期目录/Table of Contents|

壳寡糖对运动大鼠心肌组织保护作用的研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第5期
页码:
688-691
栏目:
基础研究
出版日期:
2010-06-22

文章信息/Info

Title:
Research concerning the protective effect of chitooligosaccharide on the cardiac muscle of rats with heavy load training
作者:
熊静宇; 陈筱春; 文质君
湛江师范学院体育系运动人体科学室,广东 湛江 524048
Author(s):
XIONG Jing-yu;  CHEN Xiao-chun;  WEN Zhi-jun
Department of Physical Education, Zhanjiang Normal College, Zhanjiang 524048, Guangdong, China
关键词:
壳寡糖; 大负荷运动; 心肌; 形态学; 自由基代谢; 大鼠
Keywords:
chitooligosaccharide;  heavy load training;  cardiac muscle;  morphology;  free radical metabolism;  rats
分类号:
G804
DOI:
-
文献标识码:
A
摘要:
目的: 研究壳寡糖(COS)对大负荷运动大鼠心肌组织的保护作用。方法： 将36只3月龄SPF级SD雄性大鼠随机分为正常对照组、耐力运动组和壳寡糖组，每组12只。耐力运动组和COS治疗组进行6周的跑台训练。24 h后处死大鼠，迅速取出心脏，在光镜和透射电镜下分别观察大鼠心肌组织的形态结构。测定心肌组织和血清超氧化物歧化酶(SOD)和丙二醛(MDA)的水平，SOD活性采用黄嘌呤氧化酶法测定，MDA含量采用TBA缩合比色法测定。结果： COS组大鼠心肌SOD活性（246.38±0.79）较运动组（138.61±1.17）显著增高（P<0.05），而血清SOD活性（67.92±18.47）较运动组（98.79±16.10）显著降低（P<0.05）；心肌和血清MDA含量（3.64±0.19和4.50±0.74）均较运动组（6.87±0.34和7.86±0.46）显著降低（P<0.05）。COS治疗组大鼠心肌组织和血清中SOD的活性及MDA的含量与正常对照组比较差异无显著性。光镜下观察，运动组大鼠心肌细胞出现不同程度的水肿、变性和坏死；电镜下观察，耐力运动组大鼠心肌细胞损伤明显，肌原纤维模糊、断裂甚至坏死，线粒体肿胀等有形态学改变。COS治疗组大鼠心肌损伤的程度与耐力运动组比较明显减轻，而与正常对照组比较心肌组织的形态结构无明显变化。结论： 大负荷运动可导致大鼠心肌组织、血清抗氧化功能指标改变，心肌组织损伤。COS可提高大负荷运动大鼠心肌组织的抗氧化功能，维持心肌组织的正常形态结构。
Abstract:
AIM: To investigate the protective effect of chitooligosaccharide (COS) on the cardiac muscle of rats with heavy load training. METHODS: Thirty six male rats (SPF, 3-months old) were randomly divided into three groups: normal control group, endurance exercise group and COS treatment group. Rats belonging to the endurance exercise group and COS treatment group exercised on the treadmill for 6 weeks. All rats were sacrificed and their hearts were quickly picked up 24 h after the last training. The morphological structure of the cardiac muscle of the rats was observed by light microscopy and transmission electron microscopy. SOD activity and MDA content of the cardiac muscle and blood serum were tested. SOD activity was tested by the xanthine oxidase method and MDA content was tested by TBA colorimetric condensation. RESULTS: SOD activity of the cardiac muscle in the COS treatment group (246.38±0.79) was significantly higher than the endurance exercise group (138.61±1.17) (P<0.05), but the SOD activity of the blood serum in the COS treatment group (67.92±18.47) was significantly lower than the endurance exercise group (98.79±16.10) (P<0.05). MDA content of blood serum and cardiac muscle in the COS treatment group (3.64±0.19 and 4.50±0.74) was significantly lower than endurance exercise group (6.87±0.34 and 7.86±0.46) (P<0.05). There were no significant differences in SOD activity and MDA content between COS treatment group and normal control group. There was significant myocardial cell injury in the endurance exercise rat group. The myofibril was blurred, broken and even necrosed and mitochondrial swelling and other morphological changes were observed by light microscopy. CONCLUSION: Heavy load training induced cardiac muscle injury and anti-oxidation functional change of the cardiac muscle and blood serum. COS may improve the anti-oxidant function of the cardiac muscle of rats with heavy load training and may maintain the normal morphological structure of the cardiac muscle.

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备注/Memo

备注/Memo:
收稿日期：2009-10-22.作者简介：熊静宇,实验师,硕士生Email:xjylg@126.com
更新日期/Last Update: 2010-06-22