改良的大鼠自体静脉移植模型的建立
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2010年第5期
- 页码:
- 695-698
- 栏目:
- 基础研究
- 出版日期:
- 2010-06-22
文章信息/Info
- Title:
- A novel model of rat autogenous vein graft
- 作者:
- 肖平喜1; 陈绍良2; 田乃亮1; 葛震1; 裴兆辉2; 吴敬国2; 王礼春2
- 1.南京医科大学附属南京第一医院心内科，江苏 南京 210006；2.中山大学附属第一医院心内科，广东 广州 510080
- Author(s):
- XIAO Ping-Xi1; CHEN Shao-liang2; TIAN Nai-liang1; GE Zhen1; PEI Zhao-hui2; WU Jing-guo2; WANG Li-chun2
- 1.Department of Cardiology, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing 210006, Jiangsu, China; 2.Department of Cardiology, First Affiliated Hospital, SUN Yat-Sun University, Guangzhou 510080, Guangdong, China
- Keywords:
- model; vein graft; remodeling; Doppler ultrasonography
- 分类号:
- R617
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的: 建立稳定、简便、内皮损伤小的大鼠自体静脉移植模型。方法: 以36只雄性大鼠建立改良的简易大鼠颈外静脉移植模型，采用血管超声方法，观察大鼠颈外静脉移植后的血管超声显像，手术后血管的通畅率。病理学检查移植静脉血管内膜的情况。结果: 成功地建立了改良的大鼠自体颈外静脉移植模型，手术时间较短，近期发生血栓栓塞的几率低，管腔通常率高、未发生吻合口漏，手术成功率高。超声检查可以显示移植静脉血管的通畅度及血管腔径大小，显示吻合口的延续情况。结论: 采用改良血管套（cuff）法，可建立改良的大鼠颈静脉移植模型。该模型稳定、简便、内皮损伤小，手术时间缩短。超声检查可辅助评价模型的建立，检查移植静脉血管桥是否流通畅。
- Abstract:
- AIM：To establish a stable and simple rat autogenous vein graft model with minor endothelial injury. METHODS: A novel rat model of external jugular vein graft was in 36 male rats. Vascular ultrasound was used to observe the external jugular vein before and after transplantation, postoperative patency rate of blood vessels and vascular changes. RESULTS: An improved rat autologous jugular vein graft model was successfully established with a shorter surgical time, lower risks of thromboembolism, higher lumen patency rate and lower anastomotic leakage rate. Surgical success rate was 90%. Ultrasonography clearly showed the vein graft, the size of the lumen diameter and the anastomotic continuation. CONCLUSION: A stable and simple model of rat jugular vein graft with minor endothelial injury is established by the modified cuff technique. Ultrasonography can be conveniently used at the early stage to test the patency of the surgical model.
参考文献/References
［1］Peykar S, Angiolillo DJ, Bass TA, et al. Saphenous vein graft disease［J］. Minerva Cardioangiol, 2004, 52(5):379-390.
［2］Kwei S, Stavrakis G, Takahas M, et al. Early adaptive responses of the vascular wall during venous arterialization in mice［J］. Am J Pathol, 2004, 164 (1): 81-89.
［3］ Diao Y, Xue J, Segal MS. A novel mouse model of autologous venous graft intimal hyper-plasia (1,2)［J］. J Surg Res, 2005, 126(1):106-113.
［4］Shi C, Patel A, Zhang D, et al. Plasminogen was not required for neointima formation in a mouse model of vein graft stenosis［J］. Circ Res, 1999, 84(8):883-890.
［5］ Cooley BC. Model of murine interpositional vein grafting［J］. Microsurgery, 2005, 25(3):209-212.
［6］Carmeliet P, Moons L, Stassen JM, et al. Vascular wound healing and neointima formation induced by perivascular electric injury in mice［J］. Am J Pathol, 1997, 150(2):761-774.
［7］Souza DS, Johansson B, Boj L, et al. Harvesting the saphenous vein with surrounding tissue for CABG provides long-term graft patency comparable to the left internal thoracic artery: results of a randomized longitudinal trial［J］. J Thorac Cardiovasc Surg, 2006, 132(2):373-378.
［8］Kloppenburg GT, Grauls GE, Bruggeman CA, et al. Adenoviral activin A expression prevents vein graft intimal hyperplasia in a rat model［J］. Interact Cardiovasc Thorac Surg, 2009, 8(1):31-34.
[9] Fuster V, Badimon L, Badimon JJ, et al. The porcine model for the understanding of thrombogenesis and atherogenesis［J］. Mayo Clin Proc, 1991, 66(8):818-831.
[10]Schachner T, Heiss S, Mayr T, et al. Connective tissue changes in a mous model of vein graft disease［J］. J Cardiovasc Surg (Torino), 2008, 49(2):269-276.
[11]朱海龙,杨景学,张卫达,等. 实验性自体移植静脉内膜增生模型的建立［J］. 第四军医大学学报, 2000, 21 (5):589-591.
[12]张棉根,钱虎声,邝耀麟. 实验性自本静脉移植平滑肌细胞增殖周期动力学研究［J］. 中华外科杂志, 1996, 34(7):405-408.
[13]Kilian EG, Eifert S, Beiras-Fernandez A, et al. Adeno-associated virus-mediated gene transfer in a rabbit vein graft model［J］. Circ J, 2008, 72(10):1700-1704.
[14]Popov AF, Dorge H, Hinz J, et al. Accelerated intimal hyperplasia in aortocoronary internal mammary vein grafts in minipigs［J］. J Cardiothorac Surg, 2008, 3:20.
备注/Memo
- 备注/Memo:
- 收稿日期：2009-05-27.基金项目：广东省科技攻关项目资助（2007B080701031） 通讯作者：裴兆辉，副主任医师，主要从事次声生物学效应研究Email:leak123@126.com 作者简介：肖平喜，主治医师，博士生Email:sysu-xiao@163.com
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