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|本期目录/Table of Contents|

病毒性心肌炎患者外周血T淋巴细胞亚群及B淋巴细胞的研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第1期
页码:
110-112,122
栏目:
临床研究
出版日期:
2010-10-27

文章信息/Info

Title:
Role of T lymphocyte subgroup and B lymphocytes in viral myocarditis
作者:
董新1; 张岩1; 王聪霞1; 张春艳1; 路艳1; 李永勤1; 陈俊亮2
1.西安交通大学医学院附属第二医院心内科，陕西 西安 710004；2.渭南市人民医院心内科，陕西 渭南 720032
Author(s):
DONG Xin1;  ZHANG Yan1;  WANG Cong-xia1;  ZHANG Chun-yan1;  LU Yan1;  LI Yong-qin1;  CHEN Jun-liang2
Department of Cardiology, Second Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710004, Shaanxi, China
关键词:
病毒性心肌炎; T淋巴细胞亚群; B细胞; 流式细胞术
Keywords:
T lymphocyte subgroup;  B lymphocyte;  viral myocarditis;  flow cytometry
分类号:
R542.21
DOI:
-
文献标识码:
A
摘要:
目的: 测定不同时期病毒性心肌炎(VMC)患者T淋巴细胞亚群及B淋巴细胞的数量，并同时检测患者血清心肌损害标志物心肌肌钙蛋白I(cTnI)的水平,探讨其在VMC发病机制中的作用。方法： 采用流式细胞术检测不同时期VMC患者(n=126)外周血CD4+CD45RA+和CD4+CD45RO+以及CD19+的表达,并设正常人群作为对照组(n=50)。结果： 急性期（<3个月）患者CD4+CD45RA+和CD4+CD45RO+以及CD19+与对照组相比差异显著。<1个月以CD4+CD45RA+和CD4+CD45RO+为著（P<0.01；P<0.01）。病程<7 d的患者与对照组相比，CD4+CD45RA+值的降低具有显著性(P<0.01)；CD4+CD45RO+值下降明显(P<0.01)。而病程7~14 d、14~21 d及21~30 d的患者CD4+CD45RA+值、CD4+CD45RO+值及CD19+值与对照组相比差异均有统计学意义，CD4+CD45RO+值恢复较快。CD4+CD45RA+/CD4+CD45RO+在发病14 d内的患者与对照组差异无统计学意义；而14~30 d的患者与对照组相比差异有显著性。发病1~3个月患者以CD19+为著（P<0.01）。急性期CD4+CD45RA+/CD4+CD45RO+细胞的比例失衡。恢复期（3~12个月）患者CD4+CD45RA+和CD4+CD45RO+以及CD19+、CD4+CD45RA+/CD4+CD45RO+细胞的比例与对照组相比差异无显著性。结论： VMC患者感染初期（<1个月）以T淋巴细胞亚群异常为主，感染后期（1~3个月）以B淋巴细胞异常较为显著，以上两时期均存在CD4+CD45RA+/ CD4+CD45RO+细胞比例失衡。恢复期（3~12个月）T淋巴细胞及B淋巴细胞逐渐恢复至正常。
Abstract:
AIM: To explore the role of T lymphocyte subgroup and B lymphocytes in the pathogenesis of viral myocarditis. METHODS: The quantity of CD4+CD45RA+, CD4+CD45RO+ and CD19+ in peripheral blood of viral myocarditis patients was detected using flow cytometry, and cTnI level was measured. Healthy subjects served as controls. RESULTS: The quantity of CD4+CD45RA+, CD4+CD45RO+ and CD19+ in acute patients (<1 month) was significantly different from those in controls (P<0.05). The difference of CD4+CD45RA+ and CD4+CD45RO+ was notable within 1 month (P<0.01; P<0.01). CD4+CD45RO+ decreased in the first week and CD4+CD45RA+ decreased in the second week. The difference of CD4+CD45RA+/CD4+CD45RO+ was significant from 14 to 30 days. The difference of CD19+ was notable from 1-3 months. CD4+CD45RA+/CD4+CD45RO+ cyte ratio imbalance was observed in acute stage. In recovery phase (3-12 months), the quantity of CD4+CD45RA+, CD4+CD45RO+, CD19+ and CD4+CD45RA+/CD4+CD45RO+ cyte ratio in patients was not significantly different from those in controls. CONCLUSIONS: In viral myocarditis patients, T lymphocyte subgroup abnormality and B lymphocyte abnormality play an important role, respectively, in early infection (<1 month) and in later infection (1-3 months). Th1/Th2 cyte ratio imbalance exists in acute stage (<3 months). In recovery phase (3-12 months), T lymphocyte subgroup and B lymphocytes recover gradually.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2009-03-23.作者简介：董新，副主任医师，硕士Email:Dongxin@medmail.com.cn
更新日期/Last Update: 2010-10-27