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|本期目录/Table of Contents|

脂肪间充质干细胞移植对兔心梗后近期心功能的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第2期
页码:
193-196
栏目:
基础研究
出版日期:
2010-12-10

文章信息/Info

Title:
Effect of transplantation of adipose tissue-derived mesenchymal stem cells on short-term heart function in rabbits with myocardial infarction
作者:
张卫泽1; 肖立运2; 陈永清1; 马凌1; 褚嫒嫒2
1.兰州军区兰州总医院心血管内科，甘肃 兰州 730050；2.兰州大学第二临床医学院,甘肃 兰州 730000
Author(s):
ZHANG Wei-ze1;  XIAO Li-yun2;  CHEN Yong-qing1;  MA Ling1;  CHU Ai-ai2
1.Department of Cardiology, Lanzhou General Hospital, Lanzhou Military Area Command, Lanzhou 730050, Gansu, China; 2.Second Medical College, Lanzhou University, Lanzhou 730000, Gansu, China
关键词:
脂肪间充质干细胞; 移植; 左室重构; 兔
Keywords:
ADMSCs;  transplantation;  left ventricular remodeling;  rabbits
分类号:
R542.22
DOI:
-
文献标识码:
A
摘要:
目的: 观察脂肪间充质干细胞(ADMSCs)心肌内移植对兔心肌梗死(MI)后心力衰竭近期心功能的影响，并初步探讨其机制。方法： 将30只兔随机分为假手术组（只穿线不接扎）、对照组（心肌内注射IMDM培养基）及干细胞移植组（心肌内注射ADMSCs），每组10只(n=10)；结扎兔冠状动脉左前降支（LAD）制作急性MI模型,同时将6-二氨基-乙-苯茚二酮(DAPI)标记的ADMSCs注射到梗死区数个不同部位。造模前及移植后4周、8周，用超声心动图检测心功能，术后8周处死动物测量左室体质量指数及组织学观察，采用免疫荧光方法鉴定移植的细胞。结果: 移植后4周起，心功能指标明显改善(P<0.05),术后第8 周心功能指标改善更明显(P<0.01)，左室重构程度也得到明显改善(P<0.01)。结论： 移植的ADMSCs可在宿主体内成活，并显著改善兔MI后的心功能。
Abstract:
AIM: To study the effect of transplantation of adipose tissue-derived mesenchymal stem cells (ADMSCs) on short-term heart function in rabbits with myocardial infarction (MI). METHODS: A total of 30 Japanese big ear albino rabbits were selected and randomly divided into three equal groups: sham-operation group (A), control group (B), ADMSCs transplantation group (C). MI models were established in rabbits in B and C groups. The left anterior descending (LAD) arteries of the rabbits were ligated to establish animal models of acute MI (AMI) and DAPI-marked ADMSCs were transplanted into the models. Before transplantation and 4 and 8 weeks after transplantation, heart functions were examined by ultrasonographic cardiogram test and left ventricular weight index was measured. RESULTS: Heart function improved 4 weeks after transplantation and the left ventricular weight index reduced significantly 8 weeks after transplantation. CONCLUSION: Transplanted ADMSCs can survive and improve heart functions in rabbits with heart failure after AMI.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2010-03-22.基金项目：全军医药卫生科研基金资助项目（06MB094） 作者简介：张卫泽，主任医师，博士Email：zhangweizee@163.com
更新日期/Last Update: 2010-12-10