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PPARδ基因过表达对RAW264.7细胞MCP-1及VCAM-1表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第3期
页码:
313-317，321
栏目:
基础研究
出版日期:
2011-05-12

文章信息/Info

Title:
PPAR gene transfection inhibits oxidized low-density lipoprotein-induced MCP-1 and VCAM-1 gene and protein expression in RAW264.7 cells
作者:
杨大春; 杨永健; 李德; 马双陶; 唐兵; 张鑫; 李刚
成都军区总医院心血管内科，四川 成都 610083
Author(s):
YANG Da-chun;  YANG Yong-jian;  Li De;  MA Shuang-tao;  TANG Bing;  ZHANG Xin;  LI Gang
Department of Cardiology, General Hospital, Chengdu Military Area Command, Chengdu 610083, Sichuan, China
关键词:
动脉粥样硬化; 巨噬细胞; 过氧化酶物增殖体激活受体δ; 炎症介质
Keywords:
atherosclerosis;  macrophage;  peroxisome proliferator-activated receptor δ;  inflammatory mediators
分类号:
R543.5
DOI:
-
文献标识码:
A
摘要:
目的:观察过氧化物酶增殖体激活受体δ(PPARδ)基因转染对氧化型低密度脂蛋白(ox-LDL)诱导的鼠源性单核细胞RAW264.7中单核细胞趋化蛋白-1(MCP-1)及血管细胞间黏附分子-1(VCAM-1)基因和蛋白表达的影响。方法: 构建表达人PPARδ基因的复制缺陷型腺病毒表达载体(Ad-PPARδ)，并培养RAW264.7细胞。实验分为未转染的对照组、PPARδ基因转染组及假转染组。将RAW264.7细胞与ox-LDL(50 mg/L)孵育24 h后，采用RT-PCR及蛋白免疫印迹法分别检测各组细胞MCP-1及VCAM-1 mRNA及其蛋白的表达。采用单核细胞趋化试验检测人外周血单核细胞在不同条件培养基中的趋化活性。结果: PPARδ基因转染组细胞中MCP-1及VCAM-1的表达明显低于对照组及假转染组(P<0.05)；单核细胞移动的距离明显小于对照组及假转染组(P<0.05)。假转染组与对照组比较，MCP-1及VCAM-1的表达及单核细胞移动的距离无显著差异。结论: PPARδ基因转染能抑制ox-LDL诱导的MCP-1及VCAM-1表达，增加PPARδ的表达可能具有防治动脉粥样硬化的作用。
Abstract:
AIM:To investigate whether peroxisome proliferator-activated receptor δ (PPARδ) gene transfection inhibits oxidized low-density lipoprotein (ox-LDL)-induced monocyte chemoattractant protein-1 (MCP-1) and vascular cell adhesion molecule-1 (VCAM-1) gene and protein expression in RAW264.7 cells. METHODS: A replication-deficient recombinant adenovirus expression vector of human PPARδ was constructed using the AdEasy system. RAW264.7 cells were randomly divided into three groups: control group, P group (PPARδ gene transfection group) and ST group (sham transfected group). RAW264.7 cells were incubated for 24 h with ox-LDL (50 mg/L). Changes of MCP-1 and VCAM-1 at mRNA and protein levels in different groups were detected by RT-PCR and immunoblotting. Conditioned media with or without PPARδ gene transfection were collected for chemotaxis assay by micropore filter using a modified Boyden chamber. RESULTS: MCP-1 and VCAM-1 mRNA as well as protein expressions from P group were significantly lower than in control group and ST group (P<0.01). Compared with control group and ST group, monocyte chemotactic activity was noticeably inhibited in P group (P<0.05). No significant difference was observed between control group and ST group. CONCLUSION: PPARδ gene transfection inhibits ox-LDL-induced MCP-1 and VCAM-1 expression. Activation of PPARδ may play an important role in the prevention of atherosclerosis.

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备注/Memo

备注/Memo:
收稿日期：2010-11-29.通讯作者：杨大春，主治医师， 主要从事冠心病、高血压基础与临床研究Email:yangdc71@126.com
更新日期/Last Update: 2011-03-17