我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

红景天苷对脂多糖所致急性肺损伤治疗作用的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第3期
页码:
322-325，330
栏目:
基础研究
出版日期:
2011-05-12

文章信息/Info

Title:
Therapeutic effect of salidroside on lipopolysaccharide-induced acute lung injury in rats
作者:
李莺1; 武蕾1; 赵辉1; 赵澎涛2
1.空军总医院药学部，北京 100142；2.第四军医大学病理生理学教研室，陕西 西安 710032
Author(s):
LI Ying1;  WU Lei1;  ZHAO Hui1;  ZHAO Peng-Tao2
1.Department of Pharmacy, PLA Air Force General Hospital, Beijing 100142, China; 2.Department of Pathophysiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
红景天苷; 急性肺损伤; 脂多糖
Keywords:
acute lung injury;  lipopolysaccharide;  salidroside
分类号:
R563
DOI:
-
文献标识码:
A
摘要:
目的:观察红景天苷（SDS）对脂多糖（LPS）引起的大鼠急性肺损伤（ALI）的治疗作用，并初步探讨其作用的机制。方法： 将36只SD大鼠随机分为生理盐水（NS）对照组、LPS组和LPS+SDS组(每组n=12)，通过向气管内滴注LPS建立大鼠ALP模型。采用微量注射泵向大鼠右颈外静脉注射液体并留置的给药方法，NS组和LPS组注射NS并留置4 h；LPS+SDS组先于气管内滴注LPS，0.5 h后再注射SDS并留置4 h。4.5 h后处死实验动物，取肺组织观察其形态学改变，测定肺湿/干质量的比值（W/D）、肺泡灌洗液（BALF）中蛋白的含量及肺组织匀浆中TNF-α、IL-6、IL-10、乳酸脱氢酶(LDH)和髓过氧化物酶(MPO)的含量。结果: 形态学观察表明，LPS组肺组织水肿，有点、片状出血及大量的炎性细胞浸润，肺泡间隔显著增厚，肺泡腔变窄、结构消失。LPS+SDS组的肺组织损伤明显减轻，肺组织结构趋于正常，肺泡腔及支气管腔炎细胞及渗出物与LPS组相比明显减少。LPS组肺W/D与NS组相比明显增加（P<0.05），BALF中蛋白的含量显著增加（P<0.05），肺组织匀浆中TNF-α、IL-6、IL-10、LDH和MPO的含量显著增加（P<0.05）；而给予SDS治疗后，肺W/D、BALF中蛋白含量、肺组织匀浆中TNF-α、IL-6、LDH和MPO的含量与LPS组相比均明显减少（P<0.05），IL-10含量显著增加（P<0.05）。结论: SDS对LPS所致ALI具有治疗作用，这种治疗作用可能与其通过抑制肺组织中炎症介质的作用有关。
Abstract:
AIM:To observe the role of salidroside (SDS) in the treatment of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats and to further explore the underlying mechanism by which salidroside exerts protective effects on LPS-induced ALI. METHODS: Sprague Dawley rats were divided randomly into three groups: normal saline (NS) control group, LPS group and LPS+SDS group. The animal model of ALI was established by intratracheal administration of LPS ［0.5 mg/(ml·kg)］ and microinjection pump was used to inject solution through the right external jugular vein. NS was injected in NS group and LPS group for 4 h. In LPS+SDS group, LPS was instilled intratracheally and after 0.5 h, liquid SDS ［5 mg/(ml·kg)］ was injected for 4 h. After 4.5 h, histological changes of the lungs were observed, and the left lung wet-to-dry (W/D) weight ratios and the protein content in BALF were evaluated. Meanwhile, TNF-α, IL-6, IL-10, LDH and MPO in lung homogenate were measured. RESULTS: Histological study showed congestion, edema and sequestration of inflammatory cells in the lung tissues in LPS group, but the lung injury was significantly alleviated in LPS+SDS group. W/D ratio, protein content in BALF, and contents of TNF-α, IL-6, IL-10, LDH and MPO were significantly increased in LPS group (P<0.05). SDS treatment inhibited all these increased parameters (P<0.05) and increased the content of IL-10 (P<0.05). CONCLUSION: Salidroside plays an important role in treatment of LPS-induced ALI. Its protective mechanism is correlated with the inhibition of mediators of inflammation.

参考文献/References

[1]Ware LB,Matthay MA.The acute respiratory distress syndrome[J].N Engl J Med,2000,342(18):1334-1349.
[2]于晟,时圣武,薛晓东,等.复方红景天对大鼠高原严重烧伤后早期肺脏损伤的保护作用[J].中国中医急症,2004,13(11):760-761.
[3]刘亚玲,林树新,徐明达,等.红景天对烫伤后多器官功能不全综合征作用的一氧化氮机制[J].中国病理生理杂志,2003,19(10):1379-1382.
[4]李巧云.红景天口服液对大鼠高原性肺水肿的实验研究[J].中华现代中西医杂志,2003,1(11):965-966.
[5]赵贺玲,林树新,贾斌,等.红景天甙抑制兔缺氧性肺动脉平滑肌细胞增殖的作用[J].第四军医大学学报,2000,21(2):186-189.
[6]张君岚,刘艳梅,王殿华,等.IL-10抗内毒素气管内滴注致急性肺损伤作用的实验研究[J].中国病理生理杂志,2000, 16(4):324-326.
[7]Bone RC.Sir Isaac Newton,sepsis,SIRS,and CARS[J].Crit Care Med.1996,24(7):1125-1128.
[8]Kabir K, Gelinas J P,Chen M.Characterization of a murine model of endotoxin-induced acute lung injury[J].Shock, 2002,17(4):300-303.
[9]Cox G.IL-10 enhances resolution of Pulmonary inflammatory in vivo by Promoting apoptosis of neutrophils[J].Am J Physiol， 1996， 71(6):566-571.
[10]马根顺,罗丁.红景天药理作用研究进展［J］. 中国实用医药, 2009,4(28):227-228.
[11]胡晓兰,邱水凤,梅如焕,等.红景天注射液对油酸诱导兔急性肺损伤的防治作用[J].中草药,2008,39(11):1706-1709.
[12]Hagiwara S,Iwasaka H,Hidaka S,et al.Danaparoid sodium inhibits systemic inflammation and prevents endotoxin-induced acute lung injury in rats［J］. Crit Care,2008,12(2):R43.
[13]Koike T,Nadeen Qutab M,Tsuchida M,et al.Pretreatment with olprinone hydrochloride,a phosphodiesterase III inhibitor attenuates lipopolysaccharide-induced lung injury via an anti-inflammatory effect[J].Pulm Pharmacol Ther,2008,21(1):166-171.

备注/Memo

备注/Memo:
收稿日期：2010-07-26.通讯作者：赵澎涛，讲师，主要从事呼吸与循环病理生理学的研究 Email: zhaopt@fmmu.edu.cn 作者简介：李莺，主管药师Email:hhf-1116@163.com
更新日期/Last Update: 2011-03-17