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|本期目录/Table of Contents|

睡眠剥夺对大鼠心肌损伤效应的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第5期
页码:
598-600
栏目:
基础研究
出版日期:
2011-10-25

文章信息/Info

Title:
Effects of sleep deprivation on rat myocardial damage
作者:
方 舟; 李 泱; 卢才义
解放军总医院老年心血管病研究所
Author(s):
FANG Zhou;  LI Yang;  LU Cai-yi
Institute of Geriatric Cardiology, PLA General Hospital, Beijing, 100853, China
关键词:
睡眠剥夺; 心肌损伤; 氧化应激; 炎症; 大鼠
Keywords:
sleep deprivation;  myocardial damage;  oxidative stress;  inflammation
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的:评估睡眠剥夺(SD)后大鼠心肌组织损伤程度并探讨其机制。方法： 将60只实验大鼠随机分为6组，每组10只。采用改良的多平台SD法(MMPM)建立SD模型，观察SD对心肌组织中缺血修饰白蛋白（IMA）、高敏C反应蛋白（hs-CRP）、还原型谷胱甘肽(GSH)、丙二醛(MDA)、超氧化物酶(SOD)含量的影响。结果： 与笼养组（CC组）和大平台组（TC组）相比，SD后大鼠心肌组织中IMA、hs-CRP、GSH及MDA的含量明显升高(P<0.05)，且随着SD时间的延长有明显上升的趋势；而SOD的活性随着SD时间的延长有降低的趋势。结论： SD可引起大鼠心肌发生明显的氧化应激和炎症反应，且随着氧化应激和炎症反应的加重，心肌发生进行性缺血缺氧损害。
Abstract:
AIM:To evaluate the severity of myocardial damage after sleep deprivation and to explore its mechanism. METHODS: Sixty rats were randomized into six groups (ten in each group) and sleep deprivation rat models were established by “multi-platform method” (MMPM). Effects of sleep deprivation on the contents of ischemia modified albumin (IMA), high-sensitivity C-reactive protein (hs-CRP), glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) in rat myocardium were evaluated. RESULTS: Compared with those in the normal cage control (CC) and tank control (TC) group, the contents of IMA, hs-CRP, GSH and MDA increased (P<0.05). With the elongation of sleep deprivation time, the contents of these markers increased, but the activity of SOD showed a decreasing tendency. CONCLUSION: Sleep deprivation contributes to rat myocardial oxidative stress and inflammation. With the aggravating oxidative stress and inflammation, the damaging effects of ischemia and hypoxia on myocardium become more serious.

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备注/Memo

备注/Memo:
收稿日期：2010-12-13.通讯作者：卢才义，主任医师，主要从事心律失常研究 Email：cylu2000@sina.com.cn 作者简介：方舟，住院医师，硕士生Email：fangzhou5211@sina.com
更新日期/Last Update: 2011-11-03