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增龄大鼠心肌线粒体DNA损伤及DNA修复酶γ表达的变化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第1期
页码:
23-27
栏目:
基础研究
出版日期:
2012-02-25

文章信息/Info

Title:
Expression changes in DNA polymerase γ and mitochondrial DNA damage in aging rat hearts
作者:
张 兰1; 张 毅2; 刘 娴1; 李 赫1; 顾金霞1; 肖模超1; 王晓云1
(哈尔滨医科大学：1.附属第四医院心内一科，黑龙江 哈尔滨 150021，2.附属第一医院眼科，黑龙江 哈尔滨 150001)
Author(s):
ZHANG Lan1;  ZHANG Yi2;  LIU Xian1;  LI He1;  GU Jin-xia1;  XIAO Mo-chao1;  WANG Xiao-yun1
(1.Department of Cardiovascular Medicine, Fourth Affiliated Hospital, Harbin 150021, Heilongjiang, China, 2.Eye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang, China)
关键词:
线粒体DNA; DNA修复酶; 心肌
Keywords:
mitochondrial DNA;  DNA repair enzymes;  myocardium
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的:探讨大鼠增龄过程中心肌组织DNA损伤和DNA修复酶表达的变化。方法： 从不同月龄的大鼠心肌组织中提取DNA、RNA及蛋白。采用定量多聚酶链反应(Q-PCR)检测心肌DNA损伤；用RT-PCR和Western blot检测DNA修复酶γ（Polγ）表达的变化。用ELISA法检测DNA内8羟基脱氧鸟苷（8-OHdG）的水平。结果： 随着鼠龄的增长，大鼠心肌组织中核DNA(nDNA)损伤不明显，线粒体DNA(mtDNA)损伤严重，DNA内8-OHdG的水平增加，Polγ的表达下降。结论： 随着鼠龄的增长，大鼠心肌组织中DNA修复酶Polγ的表达下降，mtDNA损伤增加。DNA损伤与DNA修复能力下降间会引起恶性循环，最终可导致DNA损伤的增加加快心脏衰老。
Abstract:
AIM:To investigate whether mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) damage increase with age in rat hearts and to explore the changes of DNA polymerase γ (Polγ) in rat hearts. METHODS: Total DNA, RNA and proteins of hearts were isolated. Damages of mtDNA and nDNA were examined with quantitative polymerase chain reaction (qPCR) assay combined with EvaGreen. Gene expressions of mRNA and protein in Polγ were detected with real-time polymerase chain reaction (RT-PCR) and Western blot analysis, and the levels of 8-hydroxy-2′-deoxy-guanosine (8-OHdG) in DNA were studied with ELISA assay. RESULTS: Oxidative DNA damage increased, primarily in mtDNA. The amount of 8-OHdG in DNA significantly increased with age and gene expression of mRNA and protein in Polγ decreased with age. CONCLUSION: The gene expression of mRNA and protein in the key BER enzymes decreases with age, which causes a decrease in the repair capability of the mtDNA and the accumulation of mtDNA damage. Increased mtDNA damage and decreased expression of BER enzymes may cause a “vicious cycle” of oxidative stress that contributes to the accumulation of mtDNA mutations and age-related cardiofailure pathogenesis.

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备注/Memo

备注/Memo:
收稿日期：2011-06-19.通讯作者：王晓云，主任医师，主要从事冠心病研究 Email：wxy@medmail.com.cn 作者简介：张兰，主治医师，硕士 Email:zhenglanheart@163. com
更新日期/Last Update: 2012-02-14