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胰岛素治疗对心肌梗死大鼠胶原重构的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第2期
页码:
154-157
栏目:
基础研究
出版日期:
2012-04-25

文章信息/Info

Title:
Effects of insulin treatment on collagen remodeling after myocardial infarction in rats
作者:
田 娜1; 付 锋1; 耿超群2; 邢文娟1; 张海锋1; 张 圆1; 高 峰1
(第四军医大学：1.生理学教研室，2.航空航天医学系五队，陕西 西安 710032)
Author(s):
TIAN Na1;  FU Feng1;  GENG Chao-qun2;  XING Wen-juan1;  ZHANG Hai-feng1;  ZHANG Yuan1;  GAO Feng1
(1.Department of Physiology, 2.Department of Aerospace Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
胰岛素; 心肌梗死; 胶原重构; 心脏功能; Ⅰ/Ⅲ型胶原; 大鼠
Keywords:
insulin;  myocardial infarction;  collagen remodeling;  cardiac function;  ratio of myocardial collagen I/III
分类号:
R458.5
DOI:
-
文献标识码:
A
摘要:
目的:研究观察胰岛素治疗对心肌梗死大鼠（myocardial infarction，MI）胶原重构的影响。方法：成年雄性SD大鼠34只，随机分为3组，即假手术(Sham)组（n=6）、生理盐水(NS)对照组（n=14）及胰岛素(ISL)治疗组［n=14，缺血20 min后，通过尾静脉以4 ml/(kg·h)的速率输入50 U/L ISL 2 h，并在缺血后1周内，每日皮下注射0.5 U/ml胰岛素1 ml/kg体质量］。分别于术后1周和4周，行心脏超声心动图(UCG)监测左室射血分数（LVEF），用氯胺T法检测心肌组织中羟脯氨酸（Hyp）的含量，用Western blot检测Ⅰ型胶原和Ⅲ型胶原蛋白的表达。结果：术后1周和4周时，对照组的LVEF值较Sham相比明显降低（P<0.01），而ISL治疗组与对照组相比，LVEF显著升高（P<0.05，P<0.01）。与Sham组相比，对照组心肌胶原的含量升高，ISL治疗组可使胶原的含量显著降低（P<0.05）。1周时，对照组的心肌Ⅰ/Ⅲ型胶原的比值增加，经ISL治疗后，Ⅰ/Ⅲ型胶原的比值明显下降（P<0.05）；4周时，对照组Ⅰ/Ⅲ型胶原的比值增加，而给予ISL治疗后Ⅰ/Ⅲ型胶原的比值无明显差异。结论：MI后大鼠心肌非梗死区组织出现胶原重构，ISL可影响该过程中心肌Ⅰ/Ⅲ型胶原的比值，从而起到改善心脏功能的作用。
Abstract:
AIM:To investigate whether insulin treatment attenuates collagen remodeling of non-infarcted myocardium after myocardial infarction (MI) in rats. METHODS: MI models were produced by ligation of the anterior descending coronary artery. Thirty four adult male rats were randomized to receive one of the following treatments: sham (n=6), saline ［4 ml/(kg·h) i.v. injection beginning 20 min before ischemia and continuing for 2 h; n=14］ and insulin treatment (50 U/L, i.v. injection following the same routine and hypodermic injection of insulin ［0.5 U/ml, 1 ml/(kg·day)］ for 7 days after ischemia surgery; n=14). Parameters of echocardiogram were compared prior to and 1 and 4 weeks after operation. Hydroxyproline concentration was detected using immunochemistry. Collagen I and collagen III expressions were measured by Western blot. RESULTS: Insulin treatment significantly improved cardiac function as evidenced by increased left ventricular ejection fraction (LVEF) at 1 and 4 weeks after MI (P<0.05, P<0.01). Cardiac remodeling decreased post-MI as evidenced by reduced collagen content (P<0.05). Western blot showed that the ratios of myocardial collagen I/III of rats 1 and 4 weeks after MI were significantly higher than those in the sham group (P<0.05). Insulin treatment reduced the ratios of collagen I/III compared with those in saline hearts 1 week post-MI (P<0.05), whereas there were no differences in the ratios of collagen I/III 4 weeks post-MI. CONCLUSION: Insulin prevents heart remodeling in non-infarcted myocardium after MI by decreasing cardiac collagen I/III ratio.

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备注/Memo

备注/Memo:
收稿日期：2011-09-27.基金项目：国家自然科学基金项目资助（30800376,30800447） 通讯作者：张圆，讲师，主要从事心血管疾病研究 Email：fmmuzhr@fmmu.edu.cn 共同通讯作者：高峰，教授，主要从事胰岛素抵抗与心血管疾病研究 Email：fgao@fmmu.edu.cn 作者简介：田娜，硕士生 Email:tianna1984.love@163.com
更新日期/Last Update: 2012-04-01