我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

胰岛素改善严重烫伤大鼠心脏功能和生存率的实验研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第2期
页码:
150-153
栏目:
基础研究
出版日期:
2012-04-25

文章信息/Info

Title:
Insulin alleviates cardiac dysfunction and improves survival rate in rats after severe scald injury
作者:
蔡晓庆1; 曹 兵2; 张利华3; 刘文冲1; 张海锋1; 李 嘉1; 高 峰1
(第四军医大学：1.生理学教研室，2.口腔医学系，陕西 西安 710032，3.唐都医院心血管内科，陕西 西安 710038)
Author(s):
CAI Xiao-qing1;  CAO Bing2;  ZHANG Li-hua3;  LIU Wen-chong1;  ZHANG Hai-feng1;  LI Jia1;  GAO Feng1
(1.Department of Physiology, 2.Department of Stomatology, 3.Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
烫伤; 胰岛素; 心脏功能; 死亡率; 大鼠
Keywords:
scald;  insulin;  stress hyperglycemia;  cardiac function;  mortality
分类号:
R977.15
DOI:
-
文献标识码:
A
摘要:
目的:探讨大鼠烫伤后早期胰岛素治疗对心脏功能和死亡率的影响。方法：将60只成年SD大鼠随机分为对照组(37℃温水处理)、烫伤组（95℃热水处理）和胰岛素治疗组（皮下注射胰岛素2.5 U/kg），每组20只（n=20），于大鼠烫伤后30 min、1 h和3 h，观察各组大鼠血糖的变化，测定烫伤后3 h心脏的功能，于大鼠烫伤后8 h，记录各组大鼠的内生存状况。结果:与对照组相比，烫伤组的血糖显著上升(P<0.01)，心脏功能明显下降(P<0.01)。早期胰岛素治疗可降低烫伤后大鼠的血糖(P<0.01)，改善大鼠心脏的功能(P<0.05)。烫伤组大鼠8 h内累积死亡率为40%，而胰岛素治疗组未出现大鼠死亡。结论：重度烫伤后，早期胰岛素治疗可改善大鼠心脏的功能，提高大鼠的生存率。
Abstract:
AIM:To investigate the effects of early insulin treatment on cardiac function and survival rate in rats after severe scald injury. METHODS: Sixty male Sprague Dawley (SD) rats were randomized into sham-operated group (in 37℃ water), scald injury group (immersing the back of rats for 15 sec and abdomen for 5 sec in 95℃ water) and insulin treatment group (s.c. injection, 2.5 U/kg). Blood glucose was determined at 0 min, 30 min, 1 h and 3 h post-scald, and cardiac function was observed at 3-h post-scald. The survival status of each rat was recorded 8 h post-scald. RESULTS: Compared with those in the control group, blood glucose soared and cardiac function were impaired in scald injury group at 3 h post-scald (n=10, P<0.01). Early insulin treatment significantly decreased blood glucose (n=10, P<0.01). Meanwhile, cardiac dysfunction was alleviated by insulin treatment (n=10, P<0.05). After early insulin treatment, post-scald survival rate was significantly improved. CONCLUSION: Early insulin treatment alleviates cardiac dysfunction and improves survival rate in rats after severe scald injury.

参考文献/References

[1]Blalock A.The importance of the local loss of fluid in the production of the low blood pressure after burns[J].Arch Surg,1931,22(4):610-617.

[2]Brooks F,Dragstedt LR,Warner L,et al.Sludged blood following severe thermal burns[J].Arch Surg,1950,61(3):387-418.

[3]Moyer CA,Coller FA,Iob V,et al.A study of the interrelationship of salt solutions, serum and defibrinated blood in the treatment of severely scalded, anesthetised dogs[J].Ann Surg,1944,120(3):367-371.

[4]van den Berghe G,Wouters P,Weekers F,et al.Intensive insulin therapy in the critically ill patients[J].N Engl J Med,2001,345(19):1359-1367.

[5]Gao F,Gao E,Yue TL,et al.Nitric oxide mediates the antiapoptotic effect of insulin in myocardial ischemia-reperfusion: the roles of PI3-kinase,Akt,and endothelial nitric oxide synthase phosphorylation[J].Circulation,2002,105(12):1497-1502.

[6]Ma H,Zhang HF,Yu L,et al.Vasculoprotective effect of insulin in the ischemic/reperfused canine heart:role of Akt-stimulated NO production[J].Cardiovasc Res,2006, 69(1):57-65.

[7]Ikezu T,Okamoto T,Yonezawa K,et al.Analysis of thermal injury-induced insulin resistance in rodents[J].J Biol Chem,1997,272(40):25289-25295.

[8]Sugita H,Kaneki M,Sugita M,et al.Burn injury impairs insulin-stimulated Akt/PKB activation in skeletal muscle[J].Am J Physiol Endocrinol Metab,2005,288(3):E585-E591.

[9]Li L,Messina JL.Acute insulin resistance following injury[J].Trends Endocrinol Metab,2009,20(9):429-435.

[10]Thorell A,Nygren J,Ljungqvist O.Insulin resistance:a marker of surgical stress[J].Curr Opin Clin Nutr Metab Care,1999,2(1):69-78.

[11]McCowen KC,Ling PR,Ciccarone A,et al.Sustained endotoxemia leads to marked down-regulation of early steps in the insulin-signaling cascade[J].Crit Care Med,2001, 29(4):839-846.

[12]Ma Y,Wang P,Kuebler JF,et al.Hemorrhage induces the rapid development of hepatic insulin resistance[J].Am J Physiol Gastrointest Liver Physiol,2003,284(1):G107-G115.

[13]Melanie GC,Robert RW.Postburn trauma insulin resistance and fat metabolism[J].Am J Physiol Endocrinol Metab,2008,294(1):E1-E9.

[14]Sourij H,Saely CH,Schmid F,et al.Post-challenge hyperglycaemia is strongly associated with future macrovascular events and total mortality in angiographied coronary patients[J].Eur Heart J,2010,31(13):1583-1590.

[15]Khairollah A,Nicholas B,Geoffrey G.Hyperglycaemia and mortality[J].J R Soc Med,2007,100(11):503-507.

[16]Dungan KM,Braithwaite SS,Prieser J-C.Stress hyperglycaemia[J].Lancet,2009,373:1798-1807.

[17]Hui S,Xin S,Heng M,et al.Acute hyperglycemia exacerbates myocardial ischemia/reperfusion injury and blunts cardioprotective effect of GIK[J].Am J Physiol Endocrinol Metab,2007,293(3):E629-E635.

[18]Gauglitz GG,Herndon DN,Jeschke MG. Insulin resistance postburn: underlying mechanisms and current therapeutic strategies[J].J Burn Care Res,2008,29(5):683-694.

[19]Horton JW.Left ventricular contractile dysfunction as a complication of thermal injury[J].Shock,2004,22(6):495-507.

备注/Memo

备注/Memo:
收稿日期：2011-08-22.基金项目：国家自然科学基金项目资助（30800447），国家自然科学基金重点项目资助（81030005） 通讯作者：李嘉，讲师，主要从事炎性介质与心血管疾病研究 Email:jiali816@fmmu.edu.cn 共同通讯作者：高峰，教授，主要从事胰岛素抵抗与心血管疾病研究 Email:fgao@fmmu.edu.cn 作者简介：蔡晓庆，硕士生 Email:xiaoqcai6@126.com
更新日期/Last Update: 2012-04-01