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Title:: Management of anesthesia and cardiopulmonary bypass in a mini-pig model

作者:: 金艳¹; 陈敏²; 金振晓¹; 郑奇军¹; 王新利¹; 赵堃¹; 崔勤¹; (第四军医大学西京医院：1.心外科，2.麻醉科，陕西西安 710032)

Author(s):: JIN Yan¹; CHEN Min²; JIN Zhen-xiao¹; ZHENG Qi-jun¹; WANG Xin-li¹; ZHAO Kun¹; CUI Qin¹; (1.Department of Cardiovascular Surgery, 2.Department of Anesthesiology, Xijing Hospital, Fourth Military University, Xi’an 710032, Shaanxi, China)

关键词:: 小型猪; 体外循环; 麻醉

Keywords:: mini-pig; cardiopulmonary bypass; anesthesia

分类号:: R654.1

DOI:: -

文献标识码:: A

摘要:: 目的:探讨小型猪体外循环(CPB)实验模型的建立和麻醉管理。方法：小型猪12头，全麻诱导插管后，应用全血冷灌方法建立中低温阻闭升主动脉的体外循环模型，并于围CPB期进行动、静脉血氧饱和度(SaO2、SvO2)、血细胞比容(Hct)及乳酸水平(Lac)测定，同时监测围CPB期血流动力学变化。结果：转流过程中SaO2、SvO2均在正常范围，Lac水平随CPB时间延长，显著升高，Hct在CPB后呈中度稀释，平均血压在CPB后自主循环恢复后经过较短时间恢复到正常范围内。结论：全血冷灌法建立的小型猪CPB模型，可做为CPB条件下开展研究的动物模型。

Abstract:: AIM:To investigate the establishment and management of anesthesia and cardiopulmonary bypass (CPB) in a mini-pig model. METHODS: Twelve mini-pigs were used in this experiment. After successful anesthesia and tracheal intubation, mediastinotomy was performed to expose the heart and great vessels. CPB was established with cannulations of ascending aorta as well as superior and inferior vena cava. The circuit was composed of a mini-type membrane oxygenator, an arterial filter and a roller pump, which was primed with 250 ml balanced solution. CPB was conducted under moderate hypothermia and hearts were arrested with antegrade blood cardioplegia infusion. Hemodynamic data, arterial and venous oxygen saturation (SaO2, SvO2), hematocrit (Hct) and blood lactate acid concentrations (Lac) were determined at different time points. RESULTS: The blood was moderately diluted according to Hct values. SaO2 and SvO2 were maintained at the normal range during the CPB period, whereas Lac increased significantly with elapsed CPB time. Mean arterial blood pressure decreased significantly compared with the pre-bypass value and returned to baseline value after CPB disconnection. All hearts were successfully arrested with cardioplegia infusion and spontaneous rhythm restored upon aortic cross-clamping removal. CONCLUSION: CPB is successfully established in this mini-pig model, which can be used for CPB-related in vitro experiments.s and R219K variant significantly modulates the HDL-C response to pravastatin in patients with AMI.

参考文献/References

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小型猪体外循环动物模型建立及麻醉管理(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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