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|本期目录/Table of Contents|

高糖对THP-1巨噬细胞ABC转运体的表达和功能的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第4期
页码:
421-425
栏目:
基础研究
出版日期:
2012-08-25

文章信息/Info

Title:
Effect of high glucose on expression and function of ABC transporters in THP-1 monocyte-derived macrophages
作者:
薛嘉虹; 魏 瑾; 董 新; 朱参战; 党寅虎; 李永勤; 牛小麟
(西安交通大学医学院第二附属医院心内科，陕西 西安 710004)
Author(s):
XUE Jia-hong;  WEI Jin;  DONG Xin;  ZHU Can-zhan;  DANG Yin-hu;  LI Yong-qin;  NIU Xiao-lin
(Department of Cardiovascular Medicine, Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an 710004, Shaanxi, China)
关键词:
高糖; ABC转运体; 巨噬细胞
Keywords:
high glucose;  ATP-binding cassette transporters;  THP-1 macrophages
分类号:
R587.1
DOI:
-
文献标识码:
A
摘要:
目的:探讨高糖对体外培养的THP-1巨噬细胞中三磷酸腺苷结合盒（ABC）转运体的表达及功能的影响。方法： 以不同浓度的D-葡萄糖干预培养的THP-1单核巨噬细胞5 d，用实时定量PCR和Western blot检测巨噬细胞中ABCG1、ABCA1 mRNA和其蛋白的表达。用酶荧光化学法检测培养基中及细胞内胆固醇的含量。结果： 高糖可抑制巨噬细胞中ABCG1的表达，但是对ABCA1的表达影响不明显。随着D-葡萄糖浓度的增加，从巨噬细胞中流出的胆固醇量减少，同时细胞内胆固醇的含量增加。结论： 高糖可抑制巨噬细胞中ABCG1的表达及功能，有助于促进巨噬细胞内脂质堆积。
Abstract:
AIM:To investigate the effect of high glucose on expression and function of ABC transporters in THP-1 monocyte-derived macrophages (MDM). METHODS: THP-1 MDM were incubated with different doses of D-glucose for 5 days. Real-time PCR and Western blotting were used to measure the expression of ABCG1 and ABCA1 and enzymatic fluorometric method was used to measure cholesterol content in cells and media after glucose-incubated macrophages were treated by LDL and HDL. RESULTS: High glucose reduced the levels of ABCG1 mRNA and protein in MDM in a dose-dependent manner. However, expression of ABCA1 was not influenced by high glucose. With the increasing glucose, cholesterol efflux regulated by HDL decreased and intracellular cholesterol accumulation increased. CONCLUSION: High glucose decreases the expression of ABCG1 and cholesterol efflux in MDM and contributes to intracellular cholesterol accumulation.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2011-11-14.基金项目：国家自然科学基金资助（81100210，81170209）； 教育部博士点新教师基金资助（1896008）；西安交通大学医学创新基金资助 作者简介：薛嘉虹，主治医师，博士Email:xjh1224@163.com
更新日期/Last Update: 2012-07-20