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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2014年第2期

页码:

138-142

栏目:

基础研究

出版日期:

2014-01-20

文章信息/Info

Title:

Interleukin-33 mediates intestinal influence on heart function in mice with heart failure

作者:

刘海涛¹; 张海锋²; 李 飞¹; 李伟杰¹; 王海昌¹

(第四军医大学：1.西京医院心血管内科，2.基础医学教育实验中心，陕西 西安 710032)

Author(s):

LIU Hai-tao¹;  ZHANG Hai-feng²;  LI Fei¹;  LI Wei-jie¹;  WANG Hai-chang¹

(1.Department of Cardiology, Xijing Hospital, 2.Department of Preclinical Medical Teaching Experiment Center, Fourth Military University, Xi’an 710032, Shaanxi, China)

关键词:

心衰; 回肠; 基因表达; IL-33; 大鼠

Keywords:

heart failure;  ileum;  gene expression;  IL-33;  rat

分类号:

Q343.1

DOI:

-

文献标识码:

A

摘要:

目的:观察心衰(HF)时胃肠道炎性基因表达的变化，并通过功能分析找出可能对心脏功能产生一定影响的炎性因子。方法： 将10只大鼠分为主动脉弓缩窄(TAC)模型组和对照组，其中TAC模型组5只，对照组5只；3个月后，通过超声证实HF模型建立成功；取材心肌和回肠组织，并分析样本中的基因表达谱。在微矩阵检测的所有基因中，选择两组在回肠样本中表达有显著差异的炎性因子基因，并采用Western blot和ELISA验证分析结果。结果： 经过统计，在TAC模型组的回肠样本中有1 467个基因表达发生变化，其中462个表达上调。进一步细化筛选条件以及查阅文献后发现，编码白介素(IL)-33的基因在TAC模型组中高表达并有功能上的意义。Western blot结果表明HF导致回肠组织中IL-33表达增高，心肌中则无明显变化，而IL-3的受体ST2L在回肠和心肌中的表达均没有显著变化。ELISA结果表明，HF导致大鼠血中IL-33含量显著增高。结论： HF可诱导大鼠回肠高表达炎症细胞因子IL-33，而IL-33可能介导了HF大鼠肠道对心功能的影响。

Abstract:

AIM:To verify the assumption that changed endocrine function of gastrointestinal tract under heart failure condition may exert some influence on cardiac performance. METHODS: We developed a transverse aortic constriction (TAC) model and sham groups in mice. After 3 months, heart failure was confirmed by echocardiography. We analyzed gene expression profiles of ileum and myocardial samples. Among all the genes detected on microarray, we selected genes whose expression was significantly different in ileum samples between sham and TAC groups. Subsequently, the functional analysis of these genes was performed and the biological functions most significant to the dataset were identified. RESULTS: Based on the established statistical approach, 1,467 genes were identified in ileum samples, of which 462 were upregulated in TAC group. After refining the screening condition and performing a literature-based search, Interleukin-33 (IL-33) gene was identified with high expression level and strong connection with the regulation of cardiomyopathy. CONCLUSION: Identification of genes that are differentially expressed in ileum of heart failure mice supports the suggestion that microarray analyses may be useful in studying the role of gastrointestinal tract in heart failure. Inflammatory IL-33 may mediate the intestinal influence on the heart function in heart failure mice. These results need further investigation and validation.

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备注/Memo

备注/Memo:

收稿日期：2013-07-22.
通讯作者：王海昌，主任医师，主要从事冠心病与心律失常诊断与治疗研究Email:wanghc@fmmu.edu.cn
作者简介：刘海涛，主治医师，博士Email：xjcardio@hotmail.com

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更新日期/Last Update: 2014-01-16