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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2015年第6期

页码:

662-665

栏目:

基础研究

出版日期:

2015-07-15

文章信息/Info

Title:

Expressive evolution of hypoxia-inducible factor-1α in pressure-overloaded rat hearts

作者:

王 婷¹; 柴春艳¹; 王 甜¹; 徐邦强¹; 赵玉兰²; 海冰峰²

（1.陕西省人民医院西院一病区，陕西 西安 710068；
2.郑州大学第二附属医院心内一科，河南 郑州 450014）

Author(s):

WANG Ting¹;  CHAI Chun-yan¹;  WANG Tian¹;  XU Bang-qiang¹;  ZHAO Yu-lan²;  HAI Bing-feng²

(1.Department of Geriatrics, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi, China；
2.Department of Cardiology, Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014, Henan, China)

关键词:

低氧诱导因子-1α;  压力过负荷; 心肌肥厚; 心力衰竭; 大鼠

Keywords:

hypoxia-inducible factor-1α;  pressure-overload;  myocardial hypertrophy;  heart failure

分类号:

R542.2

DOI:

-

文献标识码:

A

摘要:

目的 探讨压力过负荷时大鼠心肌组织低氧诱导因子(HIF)-1α、血管内皮生长因子（VEGF）表达的变化。方法 将45只雄性Wistar大鼠随机分为两组，即假手术组（15只）和模型组（30只）。采用腹主动脉缩窄法建立压力过负荷大鼠模型。分别在6周、12周后，制作两组大鼠的心肌组织切片，检测血流动力学指标以及心肌组织中HIF-1α及其靶基因VEGF的表达。结果 与假手术相比，模型组大鼠心肌组织第6周出现明显肥厚(P<0.05)；心肌组织HIF-1α及靶基因VEGF的表达明显增加，并于第12周出现心力衰竭(P<0.05)；心肌组织中HIF-1α和VEGF的表达仍较假手术组增加(P<0.05)，但较本组第6周大鼠表达明显减少(P<0.05)。结论 压力过负荷造成的大鼠心肌肥厚组织，HIF-1α及靶基因VEGF的表达明显增加，但当出现心力衰竭时，HIF-1α和VEGF的表达则明显减少。

Abstract:

AIM To evaluate the expressive evolution of HIF-1α and VEGF in pressure-overloaded rat hearts. METHODS Forty-five male Wistar rats were randomly divided into sham-operated group (n=15) and model group (n=30). Pressure-overloaded rat heart models were produced by abdominal aortic banding.After 6 weeks and 12 weeks, respectively, myocardium and hemodynamic performance were examined, and the expressions of HIF-1α and VEGF in myocardium of rats were determined. RESULTS After 6 weeks, left ventricular hypertrophy increased in model group compared with sham-operated group (P<0.05). The expressions of HIF-1α and VEGF increased significantly in the model group (P<0.05). After 12 weeks, the expressions of HIF-1α and VEGF in the model group increased compared with those in the sham-operated group (P<0.05), but the expressions decreased significantly compared with those during the 6th week in the same group. CONCLUSION When myocardial hypertrophy caused by pressure-overload exists, HIF-1α and VEGF expressions increase significantly but decrease dramatically when heart failure occurs.

参考文献/References

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备注/Memo

备注/Memo:

收稿日期：2014-10-19.
作者简介：王婷，主治医师，硕士 Email：tinasees@163.com

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更新日期/Last Update: 2015-07-23