姜黄素对不同亚型THP-1来源巨噬细胞表达炎症因子的抑制作用
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2016年第3期
- 页码:
- 258-262
- 栏目:
- 基础研究
- 出版日期:
- 2016-01-05
文章信息/Info
- Title:
- Inhibition effect of curcumin on inflammatory cytokine expressions of polarized macrophages
- Author(s):
- ZHOU Yao-yao; WEI Xiao-wei; GUO Ling-yu; XIE Qian-qian; ZHANG Tian-tian; ZHANG Jun-feng
- (Department of Cardiology, Shanghai Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201999, China)
- Keywords:
- curcumin; THP-1; macrophage polarization; inflammatory cytokines
- 分类号:
- R34
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 探讨姜黄素对M0、M1和M23种亚型巨噬细胞表达炎症因子的影响。方法 构建M0型/M1型/M2型巨噬细胞模型以及不同浓度梯度的姜黄素干预组和对照组，通过实时荧光定量PCR及酶联免疫吸附方法分别测定不同分组中TNFα、IL-6以及IL-123种炎症因子的表达。结果 实时荧光定量PCR结果显示，与对照组相比，姜黄素对3种亚型巨噬细胞TNFα、IL-6以及IL-12 mRNA的表达均具有明显抑制作用(P<0.01)，酶联免疫吸附实验也证实姜黄素能抑制以上3种细胞炎症因子的分泌水平(P<0.01)。结论 姜黄素对M0、M1和M23种亚型的巨噬细胞炎症因子表达水平均有不同程度的抑制作用，且呈剂量依赖关系。
- Abstract:
- AIM To investigate the effects of curcumin on the inflammatory cytokine expressions of M0, M1 and M2 macrophages. METHODS THP-1 cells were differentiated to M0, M1 and M2 macrophages and then were treated separately by different doses of curcumin (0 μmol/L, 7.5 μmol/L, 15 μmol/L, 30 μmol/L). Using real-time reverse transcription polymerase chain reaction (real-time PCR) and ELISA assay, the expressions of TNFα, IL-6 and IL-12 in each group were detected. RESULTS Compared with those in the control group, cytokine protein and mRNA expressions of TNFα, IL-6 and IL-12 were suppressed by curcumin in M0, M1 and M2 macrophages (P<0.01). Protein expression and mRNA expression both reduced with the increasing of the concentration of the curcumin, respectively. CONCLUSION Curcumin could remarkably suppress the synthesis of cytokine expressions of M0, M1 and M2 macrophages in a dose-dependent manner. Further studies are needed to verify the molecular mechanism of curcumin on macrophage polarization.
参考文献/References
[1]Pello O M,Silvestre C,De Pizzol M,et al.A glimpse on the phenomenon of macrophage polarization during atherosclerosis[J].Immunobiology,2011,216(11):1172-1176.
[2]Stoger JL,Gijbels MJ,van der Velden S,et al.Distribution of macrophage polarization markers in human atherosclerosis[J].Atherosclerosis,2012,225(2):461-468.
[3]陆文彬,沈成兴.细胞因子在单核细胞致动脉硬化中的作用[J].心脏杂志,2011,23(2):266-269.
[4]Hasan ST,Zingg JM,Kwan P,et al.Curcumin modulation of high fat diet-induced atherosclerosis and steatohepatosis in LDL receptor deficient mice[J].Atherosclerosis,2014,232(1):40-51.
[5]段维勋,杨 阳,金振晓,等. 姜黄素后处理通过JAK2/STAT3信号通路拮抗心肌缺血/再灌注损伤[J].心脏杂志,2012,24(3):298-302.
[6]Shishodia S.Molecular mechanisms of curcumin action: gene expression[J].Biofactors,2013,39(1):37-55.
[7]Hirata Y,Tabata M,Kurobe H,et al.Coronary atherosclerosis is associated with macrophage polarization in epicardial adipose tissue[J].J Am Coll Cardiol,2011,58(3):248-255.
[8]Nakagawa K,Zingg JM,Kim SH,et al.Differential cellular uptake and metabolism of curcuminoids in monocytes/macrophages: regulatory effects on lipid accumulation[J].Br J Nutr,2014,112(1):8-14.
[9]Min KJ,Um HJ,Cho KH,et al.Curcumin inhibits oxLDL-induced CD36 expression and foam cell formation through the inhibition of p38 MAPK phosphorylation[J].Food Chem Toxicol,2013,58:77-85.
[10]Guimaraes MR,Leite FR,Spolidorio LC,et al.Curcumin abrogates LPS-induced pro-inflammatory cytokines in RAW 264.7 macrophages. Evidence for novel mechanisms involving SOCS-1,-3 and p38 MAPK[J].Arch Oral Biol,2013,58(10):1309-1317.
[11]Lee KH,Chow YL,Sharmili V,et al.BDMC33,A curcumin derivative suppresses inflammatory responses in macrophage-like cellular system: role of inhibition in NF-κB and MAPK signaling pathways[J].Int J Mol Sci,2012,13(3):2985-3008.
[12]Chen F,Guo N,Cao G,et al.Molecular analysis of curcumin-induced polarization of murine RAW264.7 macrophage[J].J Cardiovasc Pharmacol,2014,63(6):544-552.
备注/Memo
- 备注/Memo:
- 收稿日期：2015-03-25.
通讯作者：张俊峰，主任医师，主要从事冠心病综合防治研究 Email：jfzhang_dr@163.com
作者简介：周瑶瑶，硕士生 Email：joyoyo88@163.com
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