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循环式血液灌注离体兔心缺血模型的制备及实验观察(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
1995年第3期
页码:
149-152
栏目:
论著
出版日期:
1995-05-01

文章信息/Info

Title:
The preparation and experimental study of an ischemic model of circulating blood perfusion in isolated rabbit hearts
作者:
张英民 曾祥龙
第四军医大学西京医院麻醉科 西安 710032
Author(s):
Zhang Yingmin; Zeng Xianglong
Department of Anesthesio1ogy，Xijing Hospital，Fourth Military Medical University，Xi’an 7l0032
关键词:
离体心脏; 心肌再灌注损伤; 丙二醛; 冠状动脉循环; 血管阻力; 肌酸磷酸激酶; 心律失常; 兔
Keywords:
isolated heart; myocardial reperfusion injury; coronary circulation; vascular resistance; creatine phosphokinase; arrhythmia; malondialdehyde
分类号:
-
DOI:
-
文献标识码:
-
摘要:
离体兔心循环式血液灌注90 min，随着灌注时间延长，心肌肌酸磷酸激酶释放量、冠脉流量及冠脉阻力均无明显变化；灌注过程中无室性心律失常发生；灌注90 min的心肌含水量与非灌注组比较，无显著差异，说明该模型的稳定性较好。心肌缺血15 min后，再灌注5 min时，血中丙二醛含量和肌酸磷酸激酶释放量有所增加；随着再灌注时间延长，冠脉流量逐渐减少，冠脉阻力进行性增高；再灌注期间室性心律失常的发生率明显高于非缺血组；再灌注的心肌含水量也明显高于非缺血组。可见，此种缺血再灌注离体兔心模型，能较好地反映心肌缺血再灌注损伤引起的病理生理变化。
Abstract:
Some isolated rabbit hearts were perfused for 90 min，no significant changes were found in creatine phosphokinase(CPK)，coronary flow and coronary vascular resistance，and ventricular arrhythmias did not occur．Compared with the unperfused group，the change of myocardial water content after perfusion of 90 min was．insignificant．These results indicated that the perfusion model was stable．After 15 min’s myocardial ischemia and 5 min’s reperfusion，the malondialdehyde(MDA)and CPK content in plasma began to increase．When perfused for 30 and 60 min，MDA and CPK increased significantly．With the prolongation of reperfusion time，the coronary flow decreased and coronary vascular resistance increased gradually．The water content in myocardium was higher in the ischemic rabbits reperfused for 60 min than in the unischemic group．During perfusion，the incidence of ventricular arrhythmias was a1so higher than in the unischemic group．The results indicated that obviously,lipid peroxidation had happened and induced a series of pathologic damage．This model，therefore，can satisfactorily show that damage caused by myocardial ischemic-reperfusion induces pathologic changes．

参考文献/References

［1］Michio F．Hideo I．Tetsuga A．In vitro assessment of myocardial function using rabbit heart．J Pharmacd Meth 1985;14:49.

［2］张丽丽．磷酸肌酸激酶测定．上海市医学化验所主编.临床生化检验(上册)．上海：科学技术出版社．1982；337.

［3］齐风菊,周 玫,陈 瑷,等．血浆丙二醛含量测定方法．第一军医大学学报 986；6：152.

［4］Hou D．Liu WY．Fang FZ．et al．The experimental study of prevention of myocardial reperfusion injury with free radicalwith free radical eliminant.Perfusion 1988;3:205.

［5］Myers CL．Weiss SJ．Kirsh MM．et al．Effects of supplementing hypothermic crystalioid cardioplegic solution．with catalase superoxide．allopurind．deferoxamine on functional recovery of glolally ischemic and reperfused isolated hearts.J Thorac Cardiovasc Strrg 1986：91：281.

［6］Guilbeau EJ．Moore LK．Viole AJ．et al．Effect of intermittent infusion of glucose-containing crystalioid cardioplegic solution on myocardial tissue lactic acid and recovery of contractility．J Thorac Cardiovasc Strrg 1984；87：920.

［7］Maloney JV．Nelson RL．Myocardial preserration during cardiopulmonary by pass.J Thorac Cardiovasc Strrg 1975；70：1040.

［8］张海林,傅绍莹,李蕴山.家兔工作心脏及两种缺血模型的制备法及实验观察．中国药理学通报1989,5：53.

备注/Memo

备注/Memo:
Received 1995-06-13
更新日期/Last Update: 1995-05-01