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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

1999年第2期

页码:

88-90

栏目:

论著

出版日期:

1999-03-01

文章信息/Info

Title:

Distribution and function of alpha adrenoceptors subtypers in portal vein of rats

作者:

保庭毅¹　吴金生¹　何泽生¹　臧益民²　牛国保²

1第四军医大学唐都医院普通外科　西安 710038　2第四军医大学生理学教研室

Author(s):

Bao Tingyi1; Wu Jinsheng1;  He Zesheng1;  Zang Yimin2;  NiuGuobao2

1Deparment of General Surgery, Tangdu Hospital, 2Department of Physiology, Fourth Military Medical University, Xi′an 710038

关键词:

受体;  肾上腺素能α　门静脉　大鼠

Keywords:

receptors;  adrenergic α portal vein rats

分类号:

-

DOI:

-

文献标识码:

-

摘要:

目的:探讨门静脉α肾上腺素受体及亚型的分布及其对门脉循环的调控机制。方法:采用药理受体分析方法, 结合离体和整体实验,测定α肾上腺素受体拮抗剂和激动剂应用前后;pA2, pD2, EC50, Rmax和门静脉压力(PVP)。结果: ①NE浓度-收缩曲线均右移, 程度为哌唑嗪(Pra)40倍, 硝苯吡啶(Nif)231.8倍, 育亨宾(Yoh)4.2倍。②对Rmax的抑制率:Nif89.1% , Pra 71.2% , Yoh41.9%。③与门静脉A受体亲和力(pA2) : Pra81.9, Yoh6.09,Nif6.02。④对PVP影响: 苯福林(Phe)显著升高PVP(P<0.05),Pra和Nif显著降低PVP(P<0.01)。结论:大鼠静脉α受体亚型分布α1亚型,而α1受体亚型构成中主要以α1a为主。它们介导门静脉的收缩效应, 调节门脉循环。

Abstract:

Aim: To investigate the distribution of Aadrenoceptor subtypes in portal vein and mechanism of α-adrenoceptor regulating on portal circulation.Methods:According to the effect in vivo and in vitro after agonist or an tagonist on NE dose response effects of preparations, the pA 2,pD2, EC50, Rnax and PVP were calculated by using pharmacological methods.Results: ①NE dose response curves were shifted to the right; Pra(40 fold) , Yoh(4.2 fold) and Nif(231 fold) ; ②The suppression of Rmax; Pra(71.2%) , Yoh(41.9%) and Nif(89.1%) ; ③pA2, Pra(8.19) , Yoh(6.09)and Nif (6.02);④Effect of Phe, Pra and Nif on PVP: The PVP was increased by Phe(P<0.01)and decreased by Pra and Nif(P<0.01).Conclusion:In portal vein of rat, the distribution of αa-drenocepter subtypes were α1 type and α1a type.these receptor smediated the contraction and regulated the portal circulation.

参考文献/References

［1］Minneman KP,Abel PW.Relationship between α1-adrenoceptor density and functional responses of rat vas deferens.Naunyn Shmedebergs Arch Pharmcol, 1984; 327 (2) : 238.

［2］Digges KG, Summers RJ. Characterization of postsynaptic α-a-drenoceptors in rat ao rtic strips and portal veins.Br J Pharmcol, 1983; 79 (4) : 655.

［3］Han CD, Abel PW , Minneman KP.α1-adrenoceptor subtypes linked to different mechanisms for increasing intracellular Ca2+in smooth muscle.Nature, 1987; 329 (24) : 333.

［4］韩启德.肾上腺素受体研究进展.生理科学进展, 1995; 26 (2) :103.

［5］Albillos A ,Dagan JCG, Iborra J , et al.Propranolol plus prazosin compared with propranolol plus isosorbide-5-mononotrate in the treatment of portal hypertension.Gastroenterology , 1998; 115 (1) : 116.

备注/Memo

备注/Memo:

(收稿1999-02-14)

常用功能

更新日期/Last Update: 1999-03-01