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精氨酸加压素诱导大鼠心脏成纤维细胞胶原合成的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2000年第3期
页码:
169-171, 173
栏目:
论著
出版日期:
2000-05-25

文章信息/Info

Title:
Effects of arginine vasopressin on collagen synthesis in rat cardiac fibroblasts
作者:
杨学东;  赵连友;  李雪;  徐海丽;  乔怀宇;  彭育红;  陈永清;  范延红
第四军医大学唐都医院心脏内科, 陕西 西安 710038
Author(s):
YANG Xue-dong; ZHAO Lian-you;  LI Xue;  XU Hai-li;  QIAO Huai-yu;  PENG Yu-hong;  CHEN Yong- qing;  FAN Yan-hong
Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi, China
关键词:
精氨酸加压素; 胶原合成; 心脏成纤维细胞; 大鼠
Keywords:
arginine vasopressin;  collagen synthesis;  cardiac fibroblasts;  rat
分类号:
R329.28
DOI:
-
文献标识码:
A
摘要:
目的 探讨精氨酸加压素（AVP）对大鼠心脏成纤维细胞（CFs）胶原合成的影响。方法 采用胰酶消化法分离、培养新生SD大鼠CFs，3H-脯氨酸掺入法和羟脯氨酸比色法分别观察不同浓度AVP及其V1受体拮抗剂［d(CH2)5Tyr2(Me)］AVP对CFs的影响。结果 ① CFs 3H-脯氨酸掺入率随着AVP浓度的增加而增高，其中10-7mol/L,10-6mol/L AVP组的3H-脯氨酸掺入率分别为（541±96）cpm/5000 cells和（565±72）cpm/5000 cells，较对照组（166±31）cpm/5000 cells明显增高（均P＜0.01）；10-7mol/L AVP+10-7mol/L［d(CH2)5Tyr2(Me)］AVP组3H-脯氨酸掺入率（268±64）cpm/5000 cells较10-7mol/L AVP组明显降低（P＜0.01）。②CFs培养上清光吸收值（A值）随着AVP浓度的增加而增高，其中10-7mol/L,10-6mol/L AVP组的培养上清A值分别为0.22±0.01和0.24±0.01，明显高于对照组0.20±0.01，有统计学意义（均P＜0.01）；10-7mol/L AVP+10-7mol/L［d(CH2)5Tyr2(Me)］AVP组的A值为0.19±0.01，明显低于 10-7mol/L AVP组（P＜0.01）。结论 AVP促进SD大鼠CFs胶原合成，其作用可能与V1受体介导有关。
Abstract:
AIM Arginine vasopressin(AVP) modulate cardiac fibroblasts(CFs) collagen synthesis was tested. METHODS CFs of neonatal SD rat were isolated by trypsin digestion method and growth-arrested CFs were stimulated with 25 ml/L fetal calf serum in the presence of varying concentrations of AVP and 10-7mol/L V1 receptor antagonist ［d(CH2)5Tyr2(Me)］AVP. Collagen synthesis was measured by 3H-proline incorporation. Collagen content of CFs culture medium was estimated by hydroxyproline chromatogragy. RESULTS ①AVP increases 3H-proline incorporation in CFs in a concentration-dependent manner. 3H-proline incorporation value of 10-7mol/L and 10-6 mol/L AVP group(541±96 cpm/5000 cells, 565±72 cpm/5000 cells, respectively) were higher than that of control group(both P＜0.01). 3H-proline incorporation value of 10-7mol/L AVP+10-7mol/L ［d(CH2)5Tyr2(Me)］AVP group(268±64 cpm/5000 cells) was lower than that of 10-7mol/L AVP group(P＜0.01). ②AVP increases A value of CFs culture medium in a concentration-dependent manner. A value of 10-7mol/L and 10-6mol/L AVP group (0.22±0.01, 0.24±0.01, respectively) were higher than that of control group 0.20±0.01 (both P＜0.01). A value of 10-7mol/L AVP+10-7mol/L ［d(CH2)5Tyr2(Me)］AVP group(0.19±0.01) were lower than that of 10-7mol/L AVP group(P＜0.01). CONCLUSION AVP increases collagen synthesis in rat CFs, which seems to be mediated through V1 receptor.

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备注/Memo

备注/Memo:
收稿日期：2000-02-09.
更新日期/Last Update: 2000-05-25