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陕西地区老年动脉粥样硬化群体Rb基因XbaI酶切位点多态性研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2000年第3期
页码:
196-197, 200
栏目:
论著
出版日期:
2000-05-25

文章信息/Info

Title:
Rb gene XbaI polymorphism in relation to atherosclerosis in Shaanxi elder population
作者:
刘军1;  舒青2;  郑强荪1;  杜日映1;  张宁仔1
第四军医大学: 1 唐都医院心脏内科, 2 生物学教研室, 陕西西安710038
Author(s):
LIU Jun1; SHU Qing2; ZHENG Qiang-sun1; DU Ri-ying1; ZHANG Ning-zi1
1Department of Cardiovasology, Tangdu Hospital, 2Department of Biology, Fourth Military Medical University, Xi'an 710038; Shaanxi, China
关键词:
动脉粥样硬化;  R b 基因;  Xba I;  限制性片段长度多态性
Keywords:
atherosclerosis;  Rb gene;  XbaI;  restrict fragment length polymorphism
分类号:
R394.5
DOI:
-
文献标识码:
A
摘要:
目的 探讨陕西地区老年动脉粥样硬化(AS)群体Rb基因内含子17多态性分布，及其该位点与AS遗传易感性的关系。方法 应用PCR对100例老年AS患者及100例对照组老年人Rb基因内含子17 XbaI酶切位点的限制性片段长度多态性(RFLP)进行对比分析。结果 在AS组及对照组中该位点共检出2种等位基因片段 945 bp(S1)及630 bp加315 bp(S2). AS组基因频率S1 0.46，S2 0.54，对照组基因频率S1 0.42，S2 0.58；二者的等位基因频率无显著差异。结论 Rb基因内含子17 XbaI酶切位点在AS群体中稳定性较高，不易发生突变，对AS的形成、发展可能影响较小。
Abstract:
AIM To investigate the relationship between the polymorphism of Rb (intron17) in elder population of Shaanxi and atherosclerosis(AS) genetic susceptibility. METHODS The polymorphism of Rb (intron17) were examined in 100 elder AS individuals and 100 elder control samples in Shaanxi by using PCR-Rb-XbaI-RFLP. RESULTS There are 2 alletic fragments (945 bp, 630 bp+315 bp) were found in AS group and control group. S1S2 genotype was the most frequent one in the two populations. Allele frequency of AS group is S1 0.46, S2 0.54 and that of control group is S1 0.42,S2 0.58. Allele frequency was no differences in the two groups (P＞0.05). CONCLUSION XbaI site of Rb (intron17) could have been certainly stable in AS population, we infered it was not liable to cause mutation, and the locus might not make a notable impact to the occurrence and development of AS.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2000-03-27.基金项目：国家自然科学基金　No. 39700165
更新日期/Last Update: 2000-05-25