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血小板衍化生长因子对血管平滑肌细胞增殖及胶原蛋白合成的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第2期
页码:
90-93
栏目:
论著
出版日期:
2001-03-01

文章信息/Info

Title:
Effects of platelet-derived growth factor on proliferation and collagen protein synthesis of vascular smooth muscle cells
作者:
任雨笙1 ; 崔 芳2 ; 贾国良2 ; 俞世强2 ; 汤朝武2; 吴宗贵1
1.第二军医大学长征医院心内科，上海200003；2．第四军医大学西京医院
Author(s):
REN Yu-sheng1 ; CUI Fang2; JIA Guo liang2 ; YU Shi-qiang2; TANG Chao-wu2;  Zong-gui1
Department of Cardiology，Changzheng Hospital，Second Military Medical University,Shanghai 200003,China; 2．Xingjing Hospital，Fourth Military Medical University
关键词:
血小板衍化生长因子; 血管平滑肌细胞; 增殖; 胶原蛋白合成; 兔
Keywords:
platelet-derived growthfactor; vascular smoothmuscle cell; proliferation; collagen protein synthesis; rabbit
分类号:
R329.28
DOI:
-
文献标识码:
A
摘要:
目的 观察血小板衍化生长因子(PDGF)对培养的血管平滑肌细胞(VSMC)增殖及胶原蛋白合成的影响。方法 采用培养的免动脉VSMC，应用 H-TdR的 H-脯氨酸掺入方法，观察PDGF-BB对免VSMC DNA合成以及胶原蛋白合成的影响。结果 PDGF-BB可促进处于静止状态的兔VSMC DNA及胶原蛋白的合成，并呈现明显的浓度依赖关系，在40 ug／L的浓度时DNA及胶原蛋白的合成达到高峰，DNA及胶原蛋白分别处于36 h和48 h合成最为显著。结论 PDGF-BB可明显促进培养的VSMC增殖及胶原蛋白的合成。
Abstract:
AIM To investigate the effects of platelet-derived growth factor(PDGF)on proliferadon and coilagen protein synthesis of vascular smooth muscle cells(VSMC)．METHODS In the present experiment，the rabbit VSMC were cultured and effects of PDGF-BB on DNA synthesis and collagen protein synthesis of rabbit VSMC were observed by using H-TdR incorporation and H-proline intorporation in vitro．RESULTS PDGF-BB could significantly promote DNA synthesis and collagen protein synthesis of quiescent rabbit VSMC in a dose-dependent manner,with a maximal response at a concentration of 40 ug／L at 36 h and 48 h respectively．CONCLUSION PDGF-BB could promote proliferation and collagen protein synthesis of cultured rabbit VSMC．

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：1999-12-22.
更新日期/Last Update: 2001-03-01