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比索洛尔对缺氧／复氧内皮细胞一氧化氮生成的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第2期
页码:
93-94，97
栏目:
论著
出版日期:
2001-03-01

文章信息/Info

Title:
Effects of Bisoprolol on nitric oxide production by endothelial cells during anoxia／reoxygenation
作者:
徐凯; 贾国良; 张荣庆; 李 飞; 刘县; 李伟
第四军医大学西京医院心内科，陕西西安71003
Author(s):
XU Kai;  JIA Guo-liang;  ZHANG Rong-qing;  LI Fei; LIU Bing;  LI Wei
Department of cardiology，Xijing Hospita1．Xi an Shaanxi 710032，China
关键词:
比索洛尔 缺氧／复氧; 内皮; 一氧化氮
Keywords:
bisoprolol; anoxia／reoxygenation; endothelium; nitric oxide
分类号:
R364.4
DOI:
-
文献标识码:
A
摘要:
目的 观察比索洛尔对培氧的人脐静脉内皮细胞(HUVECs)在缺氧／复氧(A／R)过程中一氧化氮(N0)生成的影响。方法 将HUVECs暴露于缺氧环境中30 min后复氧，井加人比索洛尔(1．50和500 umol/L)或空白对照,测量复氧前、复氧后1和4 h培养液上清NO含量。结果 对照组NO产量在复氧后1 h，4 h与复氧前相比有显著下降(均P0．05)，而与对照组和1umol/L组同一时间相比均有显著增加(均P
Abstract:
AIM To investigate the effects of bisoprolol on nitric oxide(NO)production by cultured human umbilical vein endothelium cells(HUVECa)during anoxia／reoxygenation(A／R)．METHODS After 30 min period of anoxia by exposing HUVECs monolayers to a chamber filled with 100% N2，reoxygenation was initiated，and meanwhile Bisoprolol(1，50，500umol／L or contro1)was added to HUVECs mooolayers．Products of NO in HUVECs supernatants were determined 0，l and 4h after reoxygenation．RESULTS In control group，level of products of NO decreased significantly 1 h(P<0．01)and 4 h(P<0．01)alter reoxygenatlon．Adding of 5Oumol／L or 500umol／L Bisoprolol resulted in maintaining of NO products (P>0.05)，and comparing to control and 1 umol／L Bisoprolol group level of products of NO increased significantly in 50umol／L and 500umol／L Bisoprolol group 1，4 h after reoxygenation(P<0．01)．CONCLUSION Bisoprolol can prevent level of endothelium-derived NO from decreasing during A／R，so NO may play a role in the mechanism by which β blockers can reduce ishemic reperfusion injury．

参考文献/References

[1]Kheper N,Rigattn C，Senevlratne C，et al.Chronic treatment with propranolol induces antioxidant changes and protects against ischemia-reperfusion injury [J]． J Mol Cell Cardio1．1997,29(12):3335.

[2]Zmudka K，Dublel J,Vanhaecke J，et al．Effects of oral pretreatment with metoprolol on left ventricular wall motion，infarct size．hemodynamlcs，and regional myocardial blood flow in anesthetized dogs during thrombotic coronary artery occlusion and reperfusion[J]. Cardiovasc Drugs Ther，1994，8(3):479.

[3]Jordan JE，Zhao ZQ，Vimen-Johansen J,et al.The role of neutrophils in myocardial ischemia-reperfusion injury[J].Cardiovasc Res,1999,43(4)：860．

[4]Inauen W，Granger DN, Meininget CJ，et al.An in vitro model of ischemia／reperfusion-induced microvascular injury[J]．Am J Physiol，1990，259:G134.

[5]Roth E，Matos G,Guarnieri C，et al.Influence of the betablocker therepy on neutrophil superoxide generation and platelet aggregation in experimental myocardial ischemia and reflow[J]．Acta Physiol Hung，l995,83(2):163．

[6]Sobey CG，Dalipram RA,Dusting GJ,et al．Impaired endothelium-dependent relaxation of dog coronary arteries after myocardial ischaemia and reperfusion: prevention by amlodipine，propranotol and allopurinpl[J]．Br J Pharmacol,1982，105(3)：557．

[7]Lefer AM，Lefer DJ. The role of nitric oxide and cell adhesion molecules on the microcirculation in ischaemia-reperfusion[J]．Cardiovasc Res ,l996，32(4)：743．

[8]Mehta JL,Lopez LM,Cox OE,et al.Alterations in nitric oxide synthase activity，superoxide anion generation,and platele aggregation in systemic hypertension，and effents of celiprolol[J]．Am J Cardiol，1984，74：801．

备注/Memo

备注/Memo:
收稿日期：2000-11-21.
更新日期/Last Update: 2001-03-01