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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2002年第1期

页码:

27-28,32

栏目:

论著

出版日期:

2002-01-01

文章信息/Info

Title:

Effect of tetrandrine on expression of apoptos is related-gene of vascular smooth muscle cells after balloon injury to vessel

作者:

李俊峡¹;  李佃贵³;  张卓立²;  李振彬³;  赵勇军³;  贾国良¹

第四军医大学西京医院: 1. 心血管内科, 2. 放射科, 陕西 西安 710032; 3. 河北医科大学基础部

Author(s):

LI Jun-xia¹; LI Dian-gui³;  ZHANG Zhuo-li²; LI Zheng-bin³;  ZHAO Yong-jun³;  JIA Guo-liang¹

1. Department of cardiology, Xijing Hospital, Fourth Military Medical Univeristy, Xian Shaanxi 710032, China

关键词:

粉防已碱;  血管平滑肌细胞;  凋亡;  基因表达

Keywords:

tetrandrine;  vascular smooth muscle cells;  proliferation;  apoptosis;  gene expression

分类号:

Q256

DOI:

-

文献标识码:

A

摘要:

目的 研究球囊损伤后血管平滑肌细胞(VSMC) 凋亡机制及粉防已碱(Tet) 对VSMC 凋亡及凋亡相关基因表达的影响。方法 采用末端脱氧核苷酸转移酶介导的三磷酸脱氧尿嘧啶缺口末端标记法(TUNEL) 和免疫组化技术检测球囊损伤内皮后VSMC 凋亡及bcl-1,bax 蛋白表达的变化。结果 球囊损伤后血管壁增生, 血管中膜和内膜出现细胞凋亡。Tet可明显促进VSMS 凋亡、降低球囊损伤后内膜及中膜厚度、增加管腔面积(P<0.05) , 促进bax 蛋白的表达、抑制bcl-2 蛋白的表达(P<0.05)。结论 bax,bcl-2 参与了球囊损伤内皮后血管平滑肌细胞凋亡的调节,Tet增加VSMC细胞凋亡, 防止血管再狭窄.

Abstract:

AIM To investigate the mechanism of vascular smooth muscle cells (VSMC) apoptosis after balloon injury. To study the effect of Tet on the apoptosis and apoptosis related-gene expression of VSMC. METHODS Apoptosis of VSMC, Bax protein and Bcl-2 protein was measured by TUNEL and immunohistochemical technique after balloon injury. RESULTS After balloon injury, the vascular was proliferated. Apoptosis of VSMC appeared in intima and media of the vascular. Tet significantly increase the apoptosis of VSMC and decrease the thickness of intima an media after ballon injury (P < 0. 05) , and also can increase the area of vascular luminal.Tet significantly increase the expression of Bax protein and decrease the expression Bcl-2 protein(P < 0. 05). CONCLUSION Bax and bcl-2 contribute to the regulation of VSMC apoptosis after balloon injury. Tet can increase the apoptosis of VSMc by the regulation of Bax and Bcl-2 gene.The Tet could significantly inhibit VSMC proliferation and prevent the restenosis of blood vessel after balloon injury.

参考文献/References

[1] Bittl JA. Advences in coronary [J]. N Engl Med , 1996, 335:1290.

[2] Kearney M. Histopathology of in-stent restenosis in patients with peripheral artery disease[J]. Circulation, 1997, 95: 1998-2002.

[3] 方宁远, 郑道声, 张世华, 等. 卡托普利对血管成形术后管壁增生影响的实验研究[J]. 中华心血管病杂志, 1994, 22: 275-278.

[4] Yu ST, Yu C,Wong HH, et al. Effect of endothelin-1 on thevascular smooth muscle cell cycle[J]. J Cardiovasc Pharmacol,1991, 17 (Suppl 7) : S239.

[5] 张永刚. 粉防已碱药理研究进展[J]. 国外医学中医中药分册,1997, 19 (4) : 6.

备注/Memo

备注/Memo:

收稿日期: 2000-11-09.

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更新日期/Last Update: 2002-01-01