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|本期目录/Table of Contents|

高效液相色谱测定大鼠血浆儿茶酚胺的含量(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第1期
页码:
9-11
栏目:
实验研究
出版日期:
2003-01-01

文章信息/Info

Title:
To detect the contents of mice plasma catecholamine by the high efficient liquid chromatography
作者:
裴兆辉; 朱妙章; 裴建明; 高瞻; 吕顺艳
第四军医大学生理学教研室,陕西 西安710032
Author(s):
PEIZhao-hui; ZHUMiao-zhang; PEIJian-ming; GAOZhan; LU‥Shun-ya
Department of Physiology,Fourth Military Medical University,Xi'an,Shaanxi 710032,China
关键词:
大鼠; 儿茶酚胺; 高效液相色谱; 电化学检测器
Keywords:
rat; catecholammine; high efficient liquid chromatography; electrochemical detector
分类号:
O652.63,Q577
DOI:
-
文献标识码:
A
摘要:
目的:改进高效液相色谱配电化学检测器检测血浆儿茶酚胺(CA)类物质含量的技术。方法:用不同的流动相条件,在体外用外标法对大鼠血浆CA含量进行检测。结果:色谱条件以流动相用双蒸水配制,脱气30min,pH2.8～3.0,检测电压0.6V,流速0.6ml/min外标法为理想检测条件。去甲肾上腺素(NE)最小检测极限1.003ng/ml,肾上腺素(E)最小检测极限1.21ng/ml。结论:本文方法简便、快速、可靠,可用于科研测定血浆NE和E。
Abstract:
AIM: To improve the technique of the high efficient liquid chromatography of mice plasma catecholamine to study the changes of plasma catecholammine (CA) content.METHODS:To measure the plasma CA content of bound stress mice labeled in vitro by the method of high efficient liquid chromatography electrochemical detector.RESULTS:Chromatography conditions were made with the flow-phas double steam water and degassed completely,pH 2.8～ 3.0,detecting voltage 0.6V,flow speed 0.6ml/min.The detected smallest limit of norepinephrine (NE)is 1.003ng/ml,the detected smallest limit of epinephrine(E) is 1.21ng/ml.This way is very idea.CONCLUSION:This measure method is simple,swift and confident;it can be used to detect the plasmas NE and E in clinic and basic research.

参考文献/References

[1] 冯清平, 尹华清, 张林, 等. 高效液相色谱电化学检测定血浆去甲肾上腺素 [J] . 中华医学检测杂志, 1998, 11(3):135-138.

[2] Lange DL, Haywood JR, Hinojosa-Laborde C. Endothelin enhances and inhibits adrenal catecholamine release in deoxycorticosterone acetate-salt hypertensive rats [J] . J Hypertens, 2000, 35(1,part2)Suppl:385-390.

[3] 钟友工, 朱禧星, 钟学礼, 等. 高效液相色谱法测定血清去甲肾上腺素及肾上腺素 [J] . 上海医科大学学报, 1987, 14(6):441-446.

[4] Missavage AE, Bush RL, kien ND, et al. The effect of clysed and topical epinephrine on intraoperative catecholamine levels [J] . J Trauma, 1998, 45(6):1074-1078.

[5] Irwin M, Thompson J, Miller C, et al. Effects of sleep and sleep deprivation on catecholamine and interleukin-2 levels in humans [J] . J Clin Endocrinol Metab, 1999, 84(6):1979-1985.

[6] 唐琴梅. 生物胺的分离和测定(上)-儿茶酚胺、5-羟基色胺及其代谢产物的分离和测定 [J] . 色谱, 1992, 10(2):82-87.

备注/Memo

备注/Memo:
收稿日期：2002-01-30.
更新日期/Last Update: 2003-01-01