我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

OxLDL诱导人血单核细胞表达SR-BImRNA与致炎细胞因子(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第4期
页码:
289-292
栏目:
实验研究
出版日期:
2003-07-01

文章信息/Info

Title:
The relation between the human peripheral blood monocyte expression of SR-BI mRNA and expression of proinflammatory cytokines mRNA induced by oxidized low density lipoprotein
作者:
雷新军; 马爱群; 耿涛; 白玲; 张葳
西安交通大学第一医院心血管内科,陕西 西安 710061
Author(s):
LEI Xin-jun;  MA Ai-qun;  GENG Tao;  BAI Ling;  ZHANG Wei
Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China
关键词:
清道夫受体; 细胞因子; 单核细胞; 脂蛋白; 岩藻多糖; 动脉粥样硬化
Keywords:
scavenger receptor;  cytokine;  monocyte;  lipoprotein;  fucoidin;  atherosclerosis
分类号:
R363.21
DOI:
-
文献标识码:
A
摘要:
目的:研究人动脉粥样硬化病变部位B族I型清道夫受体(SR-BI)表达增加的机制。方法:采用地高辛标记的寡核苷酸探针原位杂交技术,观察氧化型低密度脂蛋白(OxLDL)及A族清道夫受体(SR-A)的特异度抑制性配体-- 岩藻多糖作用下,原代培养的人外周血单核细胞SR-BImRNA表达与单核细胞趋化蛋白-1(MCP-1)、碱性成纤维细胞生长因子(bFGF)、血小板源性生长因子(PDGF)、白细胞介素-10(IL-10)四种致炎细胞因子mRNA表达之间的关系。果:OxLDL(10～30μg·ml-1)同人外周血单核细胞孵育24h后能够以剂量依赖性方式显著提高SR-BImRNA,MCP-1,bFGF,PDGF与IL-10四种致炎细胞因子mRNA的表达(均P<0.01)。岩藻多糖(50～250μg·ml-1)能够明显抑制OxLDL(20μg·ml-1)对SR-BImRNA及上述四种因子mRNA表达的诱导(均P<0.01)。结论:OxLDL可以同步刺激原代培养的人血单核细胞表达SR-BImRNA和致炎细胞因子mRNA,SR-A是介导此过程最关键的受体。
Abstract:
AIM: To investigate the mechanism of scavenger receptor class Btype I (SR-BI) upregulated in human atherosclerotic lesions. METHODS: With the digoxenin-labeled oligonucleotide-probes in situ hybridization, we investigated the relation between the primary cultured human peripheral blood monocyte expression of SR-BImRNA and expression of proinflammatory cytokines mRNA including MCP-1, bFGF, PDGF and IL-10 affected by oxidized low density lipoprotein (OxLDL) and fucoidin, a specific inhibitory ligand for scavenger receptor class A (SR-A). RESULTS: Monocyte was significantly increased the mRNA expression of SR-BI, MCP-1, bFGF, PDGF and IL-10 in a dose-dependent manner after incubating with OxLDL (10～30μg·ml-1) for 24h (P<0.01). Fucoidin (50～250μg·ml-1) distinctly inhibited OxLDL (20μg· ml-1) from inducing monocyte them RNA expression of SR-BI as well as expression of above proinflammatory cytokines (P< 0.01). CONCLUSION: OxLDL can simultaneously stimulated the primary cultured human peripheral blood monocyte expression of SR-BImRNA and mRNA expression of proinflammatory cytokines, among which SR-A plays a crucialrole.

参考文献/References

[1] Arai T, Wang N, Bezouevski M, et al. Decreased atherosclerosis in heterozygous low density lipoprotein receptor-deficient mice expressing the scavenger receptor BI transgene[J].JBiolChem,1999,274:2366-2371.

[2] Fluite rK, van der Westhuijzen DR, VanBerkel TJ. In vivo regulation of scavenger receptor BI and these lective uptake of high density lipoprotein cholesteryl esters in rat liver parenchymal and kupffer cells [J] . J Biol Chem, 1998, 273:8434-8438.

[3] Hirano K, Yamashita S, Nakagawa Y, et al. Expression of human scavenger receptor class B type I in cultured human monocyte-derived macrophages and atherosclerotic lesions [J] . Circ Res, 1999, 85:108-116.

[4] Ares MP, Kallin B, Eriksson P, et al. Oxidized LDL induces transcription factor activator protein-1 but inhibits activation of nuclear factor-kB inhuman vascular smooth muscle cells [J] . Arterioscler Thromb Vasc Biol, 1995, 15:1584-1590.

[5] Han JH, Nicholson AC, Zhou XY, et al. Oxidized low density lipoprotein decreases macrophage expression of scavenger receptorB-I [J] . J Biol Chem, 2001, 276:16567-16572.

[6] Perez FR, Kaski JC. Interleukin-10 and coronary disease [J] . Rev Esp Cardiol, 2002, 55:738-750.

[7] Lougheed M, Lum CM, Ling W, et al. High affinity saturable uptake of oxidized low density lipoprotein by macrophages from mice lacking the scavenger receptor class A type I/II [J] . J Biol Chem, 1997, 272:12938-12944.

[8] Nozaki S, Kashiwagi H, Yamashita S, et al. Reduced uptake of oxidized low density lipoproteins in monocyte-derived macrophages from CD36-deficient subjects [J] . J Clin Invest, 1995, 96:1859-1865.

[9] Ramprasad MP, Terpstra V, Kondratenko N, et al. Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein [J] . Proc Natl Acad Sci USA, 1996, 93:14833-14838.

[10] van der Kooij MA, von der Mark EM, Kruijt JK, et al. Huaman monocyte-derived macrophages express an approximately 120-kD OxLDL binding protein with strong identity to CD68 [J] . Arterioscler Thromb Vasc Biol, 1997, 17:3107-3116.

[11] Johnson WJ, Mahlerg FH, Rothblat GH, et al. Cholesterol transport between cells and high density lipoprotein [J] .Biochim Biophys Acta, 1991, 1085:273-298.

[12] Aiello RJ, Bourassa PA, Lindsey S, et al. Monocyte chemoattractant protein-1 accelerates atherosclerosis in apolipoprotein E-dagicient mice [J] . Arterioscler Thromb VascBiol, 1999, 19:1518-1525.

[13] 李莹, 曾智. 氧化型高密度脂蛋白与动脉粥样硬化 [J] . 心脏杂志, 2002, 14(5):423-425.

备注/Memo

备注/Memo:
收稿日期：2002-10-29.
更新日期/Last Update: 2003-07-01