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ClC23 基因敲除对小鼠心室肌细胞容积敏感性Cl- 电流的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第6期
页码:
485-489
栏目:
实验研究
出版日期:
2003-11-01

文章信息/Info

Title:
Effect of ClC-3 deficiency on volume-sensitive Cl-current in ventricular cardiomyocytes isolated from adult mice
作者:
龚卫琴12; 臧益民2; 王晓明12; 李源1
1.第四军医大学西京医院老年病1科, 陕西 西安 710032; 2.第四军医大学生理学教研室
Author(s):
GONG Wei-qin12;  ZANG Yi-min2;  WANG Xiao-ming12 ;  LI Yuan1
1.Department of Geriatrics, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi’an, Shaanxi 710032
关键词:
心室肌细胞; ClC-3; 氯通道; 细胞容积
Keywords:
cardiomyocytes;  ClC-3;  chloride channel;  cell volume
分类号:
R331.38　
DOI:
-
文献标识码:
A
摘要:
目的:研究小鼠心室肌细胞容积敏感性Cl- 电流与ClC23 氯通道的相关性。方法: 采用基因敲除技术获得ClC-3 基因缺失型小鼠; 膜片钳全细胞记录法记录分离的小鼠心室肌细胞低渗诱导的容积敏感性Cl- 电流。结果: ClC-3基因敲除小鼠心室肌细胞存在典型的容积敏感性Cl- 电流, 电生理学特性表现为外向整流性、高电位刺激下的时间依赖性失活, 以及受到Cl- 通道阻断剂(D IDS) 的抑制。而且, 基因敲除鼠的电流幅度与野生鼠和杂交鼠相比无明显 差异(P>0.05)。结论: ClC-3 不是编码小鼠心室肌细胞容积敏感性氯通道的基因。
Abstract:
AIM: To investigate the relationship between volume-sensitive Cl-current (ICl,vol) and ClC-3 channel in mouse ventricular myocytes. METHODS: ClC-3 deficient mice were obtained by using a knockout technique and ICl,vol in isolated mouse ventricular myocytes was recorded by using whole cell voltage clamp technique. RESULTS: Cardiomyocytes isolated from ClC-3 knockout mice had typical volume-sensitive Cl-current which exhibited moderate outward rectification, time-dependent in activation, and was inhibited by 4, 4’-diisothiocyanatostilbene-2, 2’-disulfonic acid (DIDS). Moreover, the current amplitude in knockout mice was not significantly different, compared with those in wild-type and heterozygous mice (P>0.05). CONCLUSION: ClC-3 is not a candidate for volume-sensitive Cl-channel in mouse cardiomyocytes.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2002-03-27.
更新日期/Last Update: 2003-11-01