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|本期目录/Table of Contents|

低分子肝素对血管内皮细胞舒缩功能的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第4期
页码:
295-296,300
栏目:
基础研究
出版日期:
2004-07-01

文章信息/Info

Title:
LMWH on vascular endothelial functions
作者:
龙健; 曾智
四川大学华西医院心内科，四川 成都 610041
Author(s):
LONG Jian; ZENG Zhi
Department of Cardiology,Huaxi Hospital Sichuan University,Chengdu,Sichuan 610041,China
关键词:
低分子肝素; 内皮细胞
Keywords:
low molecular weight heparin;  endothelial cell
分类号:
R331.32;R969
DOI:
-
文献标识码:
A
摘要:
目的:本实验旨在探索低分子肝素是否具有形响内皮依赖性的血管舒缩功能的作用。方法:人脐静脉内皮细胞株ECV304接种于6孔板，分两组:低分子肝素组和对照组.低分子肝素组分4种浓度:0.72 ,2,5,8U /ml.常氧条件下培养24 h后取各组上清液，以比色法测定一氧化氮(NO)、丙二醛(MDA)的含A，放免法测定内皮素(ET)的含Ae结果:常氛条件下。.72-5 U/ml的低分子肝素促进内皮分泌NO，而8 U/ml的低分子肝素抑制NO的分泌，但。.7 2^8U /ml的低分子肝素始终抑制ET,MDA生成。结论:低分子肝素可作用于内皮细胞影响其分泌NO,ET的能力，并减少MDA生成，避免氧自由基损伤内皮，保护内皮细胞。
Abstract:
AIM:To investigatew hether low molecular weigh the parincan influencev ascular endothelial function. METHODS:Human umbilical vein endothelial cells-ECV304 were passaged to 6-well dishes and divided into two groups: the LMWH group and the controll group. There were four concentrations in the LMWH group:0.72,2，5 and 8 U/ml. After the cells were cultured in normal condition for 24 hours,the medium was removed from each well. No and MDA were measured by chromatometry and ET were measured by rdioimmunoassay. RESULTS: In normal condition the advanced effects were observed on the biosythesis and release of NO in groups with 0.72-V5 U/ml LMWH. But inhibitory effects were detected with 8 U/ml LMWH. However 0.72-8 U/ml LMWH always suppressed the biosythesis and release of MDA and ET. CONCLUSION: LMWH can influence the endocrine functions of endothelial cells,inhibit the production of MDA，and protect endothelial cells from oxyegn free radicals.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2003-8-27
更新日期/Last Update: 2004-07-01