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不同诱导条件对小鼠胚胎干细胞分化为心肌细胞的实验研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第4期
页码:
297-300
栏目:
基础研究
出版日期:
2004-07-01

文章信息/Info

Title:
Study of different methods of inducing embryonic stem cells in vitro to differentiate into cardiomyocytes
作者:
钱海燕;  李庚山;  徐红新;  吕菁君;  蒋 雯;  王 晶
武汉大学人民医院心内科, 湖北武汉430071
Author(s):
QIAN Hai-yan; LI Geng-shan;  XU Hong-xin; LU Jing-jun;  JIANG Wen; WANG Jin
Cardiovascular Department, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430071,China
关键词:
胚胎干细胞;  心肌细胞;  分化
Keywords:
embryonic stem cells;  cardiomyocytes;  differentiation
分类号:
Q254;Q813.11
DOI:
-
文献标识码:
A
摘要:
目的:比较小鼠胚胎干细胞(ESC) 在不同诱导条件下分化为心肌细胞的分化比率。方法: 用鼠胚成纤维细胞(MEF) 作为饲养层促进ESC 增殖并抑制其分化, 用维甲酸(RA)、二甲基亚砜(DMSO)、转化生长因子B1 (TGF-B1)、激活素-A(activin-A) 为分化诱导剂, 采用三步法诱导ESC分化为心肌样细胞, 并比较各组分化比率。结果: 各组实验用的各种诱导剂均能诱导ESC分化为心肌样细胞, 尤以TGF-B1(2 ng/ml)、activin-A (20 ng/ml)及20% 胎牛血清(FCS) 组成的分化培养基可以使其分化比率高达88% , 显著高于其它各组(P< 0.01)。结论: 以MEF饲养层培养, TGF2B1、act ivin-A 作分化诱导剂可作为一种比较简便、稳定、高效的ESC分化条件。
Abstract:
AIM: To compare the different methods of inducing mouse embryonic stem cells (ESC)in vitro to differentiate into cardiomyocytes. METHODS: Mouse embryonic fibroblast (MEF)feeders were used to promote the growth and to inhibit the differentiation of ESC. During the inducing process, RA , DMOS, TGF-B1 and activin-A were used as inducing reagents and constituted six kinds of differentiating media. Then a three-step method was used to induce the differentiation of ESC. RESULTS: ESC could be induced in vitro to differentiate into cardiomyocytes in all groups, and the group induced by using TGF-B1 and activin-A acquired the highest differentiating ratio. CONCLUSION: TGF-B1 and activin-A were valuable reagents in inducing ESC to differentiate into cardiomyocytes.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期: 2003-05-21
更新日期/Last Update: 2004-07-01