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腺苷抑制NF-kB在肺炎大鼠心肌损伤中的作用X(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第4期
页码:
312-314,317
栏目:
基础研究
出版日期:
2004-07-01

文章信息/Info

Title:
Effect of adenosine on NF-kB activity in experimental rats with pneumonia
作者:
黄宇戈1;  谭建新1;  黄迪南2;  徐美奕3;  郑朝晖4;  黄秀兰1
广东医学院: 1. 附属医院儿科, 2. 生化教研组, 3. 同位素室, 4. 附属医院病理科, 广东湛江524001
Author(s):
HUANG Yu-ge1;  TAN Jian-xin1;  HUANG Di-nan2;  XU Mei-yu3;  ZHENG Zhao-hui4;  HUANG Xiu-lan1
1. Department of Pediatrics, Affiliated Hosipital of Guangdong Medical College, Zhanjiang,Guangdong 524001, China
关键词:
腺苷;  剂量;  肺炎;  心肌;  核因子-kB
Keywords:
Adenosine;  dosage ;  pneumonia;  myocardium ;  NF-kB
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的: 观察金葡菌感染大鼠肺炎时心肌组织核因子-kB (NF-kB) 的表达, 以及不同剂量腺苷对其影响, 以进一步探讨重症肺炎并发心肌损害的机制和腺苷治疗的疗效及理论基础。方法: SD大鼠50只被随机分为5组, 分别为正常对照组、肺炎组和腺苷治疗A、B、C 组。金葡菌经气管插管注入复制肺炎大鼠模型, 于注菌后第2、3、4 天静脉点滴腺苷治疗, 每天90min, 剂量分别为50、100、150 ug·kg- 1·min-1。第5天处死大鼠, 立即取心脏液氮保存, 心肌组织用于病理检测, 提取核蛋白用EMSA方法检测NF-kB活性。结果: ①心肌病理组织检查示肺炎组病变明显,与对照组比较差异显著(P< 0.01) , 腺苷有保护心肌作用(P< 0.01) , 并呈剂量依赖性; ②肺炎组NF-kB活性较对照组显著增高(P< 0.01) , 腺苷呈剂量依赖性地抑制心肌NF-kB 活性(P< 0.01)。结论: 本研究证实金葡菌感染大鼠肺炎可致心肌损害,NF-kB参与心肌损伤的发生和发展。外源性腺苷可通过抑制N F2JB 活性, 从而有利于炎症的减轻和保护心肌, 此为临床应用腺苷治疗重症肺炎并发心肌损害和心功能不全提供了理论依据。
Abstract:
AIM: To further investigate the role of NF-kB in the mechanism of myocardium injury casused by infectious pneumonia and the therapeutic effects of exogenous adenosine by observing the activity of NF-kB in injured myocardium and the effects of different dosages of adenosine on NF-kB activity. METHODS: Fifty rats were divided into five experimental groups at random , each group had 10 rats. Themodel of pneumonia was induced by the injection of staphylococcus aureus into the windpipe of rats. Adenosine-treated rats (A、B and Cgroup) received daily injection of adenosine at different dosages (50、100 and 150 ug·kg-1·min-1) for 3 days. All rats were killed on the fifth day. Myocardial tissues were preserved in liguid nitrogen for examination.Pathological exa mination of myocardium were done and NF-kB activity was detected by electrophoretic mobility shift assay (EMSA). RESULTS: ①Significant increase of NF-kB activity was observed in myocardium of pneumonic rats when compared with control group (P<0.01,respectively). The increased levels of NF-kB activity were positively correlated with pathological changes in injuried myocardium ( P<0.01, respectively ) ; ②NF-kB activity were dosage-dependently decreased in adenosine-treated rats (B、C groups) when compared with pneumonia group (P< 0.01, respectively) , but no significant change in Agroup (P> 0.05). CONCLUSION:According to our finding, NF-kB may be involved in the development of myocardial injury in rats with pneumonia. Exogenous adenosine can inhibits inflammation by down-regulation NF-kB activity, and protect in jured myocardium in rat with pneumonia. Our findings provide novel therapeutic evidence of adenosine in myocardial injury induced by pneumonia in clinic.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期: 2003-10-08X基金项目：广东省卫生厅资助课题（编号：卫200513）
更新日期/Last Update: 2004-07-01