VEGF对人血单核细胞基质金属蛋白酶表达及活性的影响X(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2004年第4期
- 页码:
- 308-311
- 栏目:
- 基础研究
- 出版日期:
- 2004-07-01
文章信息/Info
- Title:
- Effect of VEGF on expression and activity of MMPs of human monocytes
- Author(s):
- WANG Chang-qian; TANG Da-ming ; WANG Li-min; XIE Xiu-lan; XU Yi-min; DING Hong-yi; SONG Wei; WANG Bin-yao; HUANG Ding-jiu
- Department of Cardiology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China
- 分类号:
- 541. 4
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的: 探讨血管内皮生长因子(VEGF) 对人单核细胞源巨噬细胞(HMDM) 基质金属蛋白酶(MMPs) 的表达及其活性的影响。方法: 分别应用RT-PCR 和Western blot检测MMPs (MMP-2 和MMP-9)基因和蛋白表达,Zymography活性分析检测MMPs活性。结果: VEGF可明显增加人单核细胞源巨噬细胞(HMDM)MMP-2、MMP-9mRNA及其蛋白表达和活性。结论:VEGF可刺激HMDMMMP-2、MMP-9mRNA及其蛋白的表达, 增强其活性, 因而有可能促进动脉粥样硬化斑块内基质降解, 进而引起粥样硬化斑块破裂。
- Abstract:
- AIM: To observe the effect of VEGF on expression and activitiy of Matrix Metalloproteinase (MMPs). METHODS: The gene and protein expression of MMPs (MMP-2 and MMP-9) were respectively detected by RT-PCR and Western blot. MMPs’ activity was detected by Zymography. RESULTS: The expressions and activities of MMP-2 and MMP-9 mRNA of human monocytes were increased significantly by VEGF. CONCLUSION : As VEGF can stimulate the expressions and activities of MMP-2 and MMP-9 of human monocytes, it may increase the breakdown of matrix in lesions of atherosclerosis and enhance the lesions’vulnerability.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期: 2003-05-23X基金项目：本课题受上海市青年科技启明星计划（98QB14014）资助
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