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白细胞介素-6 对血管升压素调控心脏成纤维细胞增殖及p27 蛋白表达的干预效应(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第5期
页码:
397-399,402
栏目:
基础研究
出版日期:
2004-09-01

文章信息/Info

Title:
Intervention effects of interleukin26 on cardiac f ibroblasts proliferation and p27 expression modulated by argipressin
作者:
陈永清1; 赵连友1; 郑强荪1; 薛玉生1; 彭育红1; 杨学东1; 田建伟1; 王士雯2
1. 第四军医大学唐都医院心内科,陕西西安710038 ;2. 解放军总医院老年心血管病研究所,北京100853
Author(s):
CHEN Yong-qing1; ZHAO Lian-you1; ZHENG Qiang-sun1; XUE Yu-sheng1; PENG Yu-hong1; YANG Xue-dong1; TIAN Jian-wei1; WANG Shi- wen2
1. Department of Cardiology , Tangdu Hospital , Fourth Military Medical University , Xi’an , Shaanxi 710038 ; 2. Institute of Geriatric Cardiology , General Hospital of PLA , Beijing 100853 , China
关键词:
白细胞介素26 ; 血管升压素; 成纤维细胞; 增殖; p27 蛋白
Keywords:
interleukin26 ;  argipressin ;  fibroblasts ;  proliferation ;  protein p27
分类号:
Q71 ;R541. 3
DOI:
-
文献标识码:
A
摘要:
目的:观察白细胞介素26 (interleukin26 , IL26) 对血管升压素(argipressin , AVP) 诱导的心脏成纤维细胞(CFs) 增 殖及p27 蛋白表达的影响。方法:以培养的新生Sprague2Dawley(SD) 大鼠CFs 为实验模型,四氮唑盐(MTT) 比色法检 测细胞增殖,流式细胞分析仪(FCM) 技术测定细胞周期及p27 蛋白表达阳性率。结果: ①10 000 U/ ml IL26 作用于 CFs 48 h ,MTT法A 值(0. 38 ±0. 01) 显著高于基础状态组(0. 32 ±0. 01 , P < 0. 01) ;10 - 7 mol/ L AVP 和10 000 U/ ml IL2 6 共同作用组CFs 的A 值(0. 41 ±0. 01) 也明显高于AVP 单独作用组(0. 39 ±0. 01 , P < 0. 01) 。②IL26 作用组CFs 的 S 期细胞百分率(13. 0 %±3. 5 %) 和细胞增殖指数(PI) (29. 4 %±1. 9 %) 均高于基础状态组(7. 5 % ±1. 0 %和26. 0 % ±1. 0 % , P < 0. 01) ,G0/ G1 期细胞百分率(70. 6 %±1. 9 %) 低于基础状态组(74. 0 %±1. 0 %) ;AVP + IL26 组CFs 的S 期细胞百分率和PI 分别为18. 8 %±1. 5 %和35. 2 %±1. 6 % ,明显高于AVP 组(15. 5 %±1. 4 %和31. 4 %±1. 5 % , P < 0. 01) ,而G0/ G1 期细胞百分率(64. 8 %±1. 6 %) 明显低于AVP 组(68. 6 %±1. 5 % , P < 0. 01) 。(3) IL26 组CFs p27 蛋白表达阳性率(72. 6 %±2. 5 %) 明显低于基础状态组(78. 4 % ±1. 9 % , P < 0. 01) ;而AVP + IL26 组p27 蛋白表达 阳性率(60. 2 %±1. 7 %) 明显低于AVP 单独作用组(63. 3 %±1. 9 % , P < 0. 01) 。结论: IL26 能够增强AVP 诱导CFs 增殖的效应,p27 蛋白可能是IL26 和AVP 调控CFs 增殖共同的细胞内靶蛋白。
Abstract:
AIM: To study the intervention effects of interleukin-6 ( IL26) on cell proliferation and p27 expression in cardiac fibroblasts (CFs) modulated by argipressin (AVP) . METHODS: Neonatal Sprague-Dawley (SD) rats CFs were cultured as models. MTT assay was used to evaluate cell proliferation. Cell cycle distribution and p27 expression were determined with flow cytometre (FCM) . RESULTS : ①A value of CFs measured with MTT assay in the 10 000 U/ ml IL26 group was 0. 38 ±0. 01 , which was significantly higher than that of basal condition (0. 32 ±0. 01 , P < 0. 01) ; A value in 10 - 7mol/ L AVP + 10 000 U/ ml IL26 group was 0. 41 ±0. 01 , which was significantly higher than that of the AVP group (0. 39 ±0. 01 , P < 0. 01) . ②The percentage of CFs at stage S (13. 0 % ±3. 5 %) and proliferation index ( PI) (29. 4 % ±1. 9 %) in the IL-6 group were higher than those of the basal condition (7. 5 %±1. 0 % and 26. 0 %±1. 0 % , P < 0. 01) , commitment with a significant decrease in the percentage of stage G0/ G1 cells (70. 6 % ±1. 9 % vs 74. 0 % ±1. 0 % , P < 0. 01) ; the percentages of CFs at stage S (18. 8 % ±1. 5 %) and PI (35. 2 % ±1. 6 %) were much higher in the AVP + IL-6 group than in the AVP group (15. 5 % ±1. 4 % and 31. 4 % ±1. 5 % , P < 0. 01) ,commitment with a significant decrease in the percentage of stage G0/ G1 cells (64. 8 % ±1. 6 % vs 68. 6 % ± 1. 5 % , P < 0. 01) . ③Positive rate of p27 expression in IL-6 group (72. 6 % ±2. 5 %) was significantly lower than that of basal condition (78. 4 % ±1. 9 % , P < 0. 01) ; Moreover , positive rate of p27 expression in AVP + IL-6 group (60. 2 % ±1. 7 %) was significantly lower than that of AVP group (63. 3 % ±1. 9 % , P < 0. 01) . CONCLUSION: IL-6 can enhance CFs proliferation induced by AVP. p27 may be the same target regulatory protein of IL-6 and AVP in CFs.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2003-8-5
更新日期/Last Update: 2004-09-01