尿酸对大鼠血管平滑肌细胞增殖的影响(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2005年第3期
- 页码:
- 218-220,224
- 栏目:
- 基础研究
- 出版日期:
- 2005-05-05
文章信息/Info
- Title:
- Effect of uric acid on proliferation of rat vascular smooth muscle cells
- Author(s):
- SUN Haiou1; JI Qiuhe1; MA Heng2; HU Yuzhen2; GAO Feng2
- 1.Department of Endocrinology, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China
- 分类号:
- R543
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 观察尿酸（uric acid，UA）对动脉粥样硬化关键环节——血管平滑肌细胞增殖的影响。 方法 以体外培养的大鼠血管平滑肌细胞为实验对象，分为对照组、尿酸组和洛沙坦＋尿酸组，每组处理后用MTT法分析细胞增殖活力，流式细胞仪（flow cytometry，FCM）检测细胞增殖周期。 结果 尿酸作用后培养细胞的MTT值明显高于对照组（40 mg/L UA:0.65±0.06 ;对照：0.37±0.07, P<0.01)，并且存在剂量依赖效应；尿酸所致上述效应还存在时间依赖性；洛沙坦能部分阻断尿酸刺激血管平滑肌细胞增殖作用。流式细胞分析显示尿酸作用使血管平滑肌细胞G1期细胞比例减少，S期细胞比例增加。结论 尿酸能够促进血管平滑肌细胞增殖，存在剂量及时间依赖效应；该作用可能部分通过血管紧张素Ⅱ的受体介导。探讨氯沙坦对心脏保护作用与肝细胞生长因子（HGF）的关系。方法 实验动物为自发性高血压大鼠（SHR）36只，分别为6周龄，14周龄和24周龄。分为两组，一组为氯沙坦组（SL），另一组为对照组（SC）；以Wistar大鼠为正常对照组（WC），年龄和体质量配比，每组各6只，雌雄配对。SL组每天按30 mg/kg灌胃法喂服，对照组采用灌胃法喂服等量蒸馏水，疗程6周。实验结束后分别测量血压，左室质量，采用M30apoptosense ELISA法测心肌细胞凋亡数量及血浆，组织HGF浓度，心肌胶原容积和血管周围胶原容积。结果 治疗6周后，与未服药的对照组相比，SL组不但血压下降，同时左室质量指数降低，间质纤维化及血管周围纤维化程度减轻，心肌细胞凋亡数量减少，血浆HGF降低，伴有组织HGF浓度升高。结论 氯沙坦能够促进组织HGF分泌，恢复其自身修复能力而逆转心室重构。同时改善内皮功能，维护心肌组织与内皮的稳定性，可能有降低心脑血管病危险性的作用。
- Abstract:
- AIM To observe the effect of uric acid on the proliferation of rat vascular smooth muscle cells, the key step of atherosclerosis. METHODS Cultured vascular smooth muscle cells(VSMC) were used and divided into control group, Uric acid(UA) group(20 mg/L, 40 mg/L, 60 mg/L )and UA+losartan group. The UA (40 mg/L) group was further divided into three subgroups on the basis of different incubation times (24, 48, 72 hours) . MTT assay and flow cytometry (FCM) were used to detect the proliferation of the cells. RESULTS UA significently increased the values of MTT absorbance（40 mg/L UA:0.65±0.06, P<0.01 compared with control) in a time and dosedependent manner. This effect co uld be partly blocked by losartan (0.59±0.05，P<0.01 compared with control). FCM showed that the percentage of G1 phase cells decreased and the percentage of S phase cells increased after UA treatment. CONCLUSION UA can accelerate the proliferation of VSMC in a time and dosedependent manner, and the receptor of angiotensin Ⅱmay play a role in the effects of UA stimulated VSMC proliferation.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期：2004-07-13.基金资助:陕西省卫生厅资金资助（04D25）
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