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¡¶ÐÄÔàÔÓÖ¾¡·[ISSN:1009-7236/CN:61-1268/R]

ÆÚÊý:

2006ÄêµÚ1ÆÚ

Ò³Âë:

23-27

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2006-01-01

ÎÄÕÂÐÅÏ¢/Info

Title:

Activation of reverseª²mode NCX during metabolic inhibition pretreatment triggers the cardioprotection of preconditionin

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ÀîÊ÷׳¹; ê°ÒæÃñ¹; Íõ²¨³; Î¤¹¢Ôó¹; ºúÓñÕä¹; ÇØÏòÑô²; ÅὨÃ÷¹; Öܾ©¾ü¹

µÚËľüÒ½´óѧ£º1. »ù´¡²¿ÉúÀíѧ½ÌÑÐÊÒ£¬2. ҩѧϵ»¯Ñ§½ÌÑÐÊÒ£¬ÉÂÎ÷ Î÷°² 710032£»3. ÉÂÎ÷ʦ·¶´óѧÉúÃü¿ÆѧԺ£¬ÉÂÎ÷ Î÷°² 710062

Author(s):

LI Shu-Zhuang¹; ZANG Yi-Min¹; WANG Bo³; WEI Geng-Ze¹;  HU Yu-Zhen¹;  QIN Xiang-Yang²; PEI Jian-Ming¹;  ZHOU Jing-Jun¹

1.Department of Physiology, 2.Department of Chemistry, Fourth Military Medical University, Xi¡äan , Shaanxi 710032, China; 3. College of Life Sciences, Shaanxi Normal University, Xi¡äan , Shaanxi 710062, China

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Keywords:

sodium/calcium exchanger;  metabolic inhibition preconditioning;  cardioprotection; ventricular myocyte

·ÖÀàºÅ:

R542.2;Q256

DOI:

-

ÎÄÏ×±êʶÂë:

A

ÕªÒª:

Ä¿µÄ Ñо¿´óÊóÐÄÊÒ¼¡Ï¸°ûÔÚ´úлÐÔÒÖÖÆÔ¤´¦ÀíÖÐÄÆ/¸Æ½»»»Ì壨NCX£©·´ÏòתÔ˵ĻîÐÔ£¬ÒÔ¼°NCX·´ÏòתÔËÒÖÖƼÁÊÇ·ñ¿ÉÒÔ×èÖ¹´úлÐÔÒÖÖÆÔ¤´¦ÀíºóµÄÐļ¡±£»¤×÷Ó᣷½·¨ ø½â·¨·ÖÀëÖƱ¸¸ÆÄÍÊÜÐļ¡Ï¸°û£¬ÓÃFuraª²2/AM¸ºÔØ£¬²ÉÓÃË«¼¤·¢Ó«¹â¹âµç±¶Ôöϵͳ£¨IonOptix Photometry System£©¼ì²â¸ÆÐźţ¬Óõ¥Ðļ¡Ï¸°û¶¯ÔµÌ½²â¼¼Êõ¹Û²ìÐļ¡Ï¸°ûÊÕËõ/ÊæÕŹ¦ÄÜ£¬Ì¨ÅÎÀ¶È¾É«·¨¼ì²âϸ°û´æ»îÂÊ¡£½á¹û ÔÚ´úлÐÔÒÖÖÆÔ¤´¦Àí30 minʱ£¬NCX·´ÏòתÔ˱»¼¤»î¡£NCX·´ÏòתÔËÒÖÖƼÁKBª²R 7943£¨0.5 ¦Ìmol/L£©¿ÉÒÔÒÖÖÆ´úлÐÔÒÖÖÆÔ¤´¦Àí¶ÔÐļ¡Ï¸°ûÊÕËõ¹¦ÄܺÍϸ°û´æ»îÂʵÄ×÷Óá£NCX¼¤¶¯¼ÁE4031£¨1 ¦Ìmol/L£©¿ÉÒÔÄ£Äâ´úлÐÔÒÖÖÆÔ¤´¦Àíºó¶ÔÐļ¡Ï¸°ûÊÕËõ¹¦Äܵı£»¤×÷Óã¬ÕâÒ»×÷ÓÃÒ²¿É±»KBª²R 7943×è¶Ï¡£½áÂÛ ´úлÐÔÒÖÖÆÔ¤´¦ÀíÖУ¬NCX·´ÏòתÔ˵ļ¤»î´¥·¢ÁË´úлÐÔÒÖÖÆÔ¤´¦ÀíºóµÄÐļ¡±£»¤×÷Óã»NCXµÄÒÖÖƼÁ¿ÉÒÔ×èÖ¹´úлÐÔÒÖÖÆÔ¤´¦ÀíºóµÄÐļ¡±£»¤×÷Óá£

Abstract:

AIM To study the activity of reverseª²mode Na+/Ca2+ exchanger (NCX) during metabolic inhibition pretreatment (MIP) of rat ventricular myocytes, and to determine whether the cardioprotection of metabolic inhibition preconditioning can be prevented by reverseª²mode NCX inhibitor. METHODS Single ventricular myocytes were enzymatically isolated and loaded with Furaª²2/AM. Intracellular calcium concentration (£ÛCa2+£Ýi) was measured by IonOptix Photometry System. The activity of reverseª²mode NCX was assessed by withdrawal extracellular Na+ and the changes of £ÛCa2+£Ýi were measured. The amplitude of single myocyte contraction and cell viability were used as indices of cell injury and death, respectively. RESULTS MIP for 30 min significantly increased the activity of reverseª²mode NCX. The effects of MIP on the amplitude of single surviving myocyte contraction and cell viability were attenuated by 0.5 ¦Ìmol/L KBª²R 7943£¨KBR£©, a specific inhibitor of reverseª²mode NCX, administered before and during MIP. Pretreatment of cells with 1 ¦Ìmol/L E4031, a selective reverseª²mode NCX enhancer, mimicked the effects of metabolic inhibition preconditioning on cell contraction, which was also blocked by 0.5 ¦Ìmol/L KBR. CONCLUSION During MIP the activation of reverseª²mode NCX triggers the cardioprotection of metabolic inhibition preconditioning, which can be prevented by blocking NCX reverseª²mode.

²Î¿¼ÎÄÏ×/References

£Û1£ÝWang B, Li SZ, Wei GZ, et al. Changes of the reverse mode of Na+/Ca2+ exchanger in isolated rat ventricular myocytes subjected to metabolic inhibition£ÛJ£Ý. Chin Heart J, 2005, 17(5): 413-415.

£Û2£Ý Zhou JJ, Pei JM, Wang GY, et al. Inducible HSP70 mediates delayed cardioprotection via Uª²50488H pretreatment in rat ventricular myocytes£ÛJ£Ý. Am J Physiol Heart Circ Physiol,2001, 281:H40 - H47.

£Û3£Ý Wu S, Li HY, Wong TM. Cardioprotection of preconditioning by metabolic inhibition in the rat ventricular myocyte. Involvement of kappaª²opioid receptor£ÛJ£Ý. Circ Res, 1999, 84:1388 - 1395.

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£Û6£ÝWu DM, Lu JY, Wu BW. Class III antiª²arrhythmia drug Eª²4031 potentiates Na+/Ca2+ exchange current in rat ventricular myocytes£ÛJ£Ý. Acta Pharmacol Sin, 2000, 21: 249-252.

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£Û10£ÝEigel BN, Gursahani H, Hadley RW. ROS are required for rapid reactivation of Na+/Ca2+ exchanger in hypoxic reoxygenated guinea pig ventricular myocytes£ÛJ£Ý. Am J Physiol Heart Cric Physiol, 2004, 286: H955-H963.

£Û11£ÝIwamoto T, Pan Y, Nakamura TY, et al. Protein kinase Cª²dependent regulation of Na+/Ca2+ exchanger isoforms NCX1 and NCX3 does not require their direct phosphorylation£ÛJ£Ý. Biochemistry, 1998, 37: 17230-17238.

£Û12£ÝHilgemann DW. New insights into the molecular and cellular workings of the cardiac Na+/Ca2+ exchanger£ÛJ£Ý. Am J Physiol Cell Physiol, 2004, 287: C1167-C1172.

±¸×¢/Memo

±¸×¢/Memo:

ÊÕ¸åÈÕÆÚ£º2005-09-28.Supported by: national natural science foundation of china(No. 30400177) Correspondence to: ZHOU Jingª²jun, Ph.D.Tel:£¨029£©84774521 Email: jjzhou71@yahoo.com.

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