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Title:: Effect of cardiac chymase inhibitor on cardiac myofibrosis in spontaneously hypertensive rats

作者:: 王先梅; 赵连友; 郑强荪; 武利军; 陈永清; 牛晓琳; 黄志刚; 第四军医大学唐都医院心内科，陕西西安 710038

Author(s):: WANG Xian-mei; ZHAO Lian-you; ZHENG Qiang-sun; WU Li-jun; CHEN Yong-qing; NIU Xiao-lin; HUANG Zhi-gang; Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi′an， Shaanxi 710038, China

关键词:: 糜酶; 大鼠; 自发性高血压; 心肌纤维化; 胶原

Keywords:: chymase; rats; spontaneously hypertension; cardiac myofibrosis; collagen

分类号:: R542.23

DOI:: -

文献标识码:: A

摘要:: 目的观察心脏糜酶在自发性高血压大鼠（SHR）心肌组织胶原合成和心肌纤维化中的作用。方法应用病理检查、计算机分析结合逆转录-聚合酶链式反应等方法，检测SHR应用糜酶抑制剂组（Chy-I组）、SHR组及对照正常血压大鼠Wistar-Kyoto组（WKY组）收缩压、心肌胶原容积分数（CVF）、心肌血管周围胶原面积比（PVCA）和心肌糜酶及I、III型胶原mRNA表达。结果 Chy-I组心脏CVF、PVCA分别为（27±9）%和0.4±0.1，SHR组分别为（46±8）%和1.9±0.9，WKY组为（24±11）%和0.4±0.1，ChyI组比SHR组显著下降（P＜0.01），与WKY组无显著差异。ChyI组心肌组织I、III型胶原和糜酶mRNA表达相对含量均显著低于SHR组（P＜0.01），与WKY组无显著差异。应用ChyI后大鼠血压与SHR比较，无显著改变。结论心肌组织糜酶参与胶原的合成，参与细胞外基质的形成和降解，促进SHR心肌纤维化，糜酶抑制剂可能对改善心肌纤维化有益。

Abstract:: AIM To evaluate the role of cardiac chymase on collagen synthesis of myocardial tissue and cardiac myofibrosis in spontaneously hypertensive rats. METHODS Pathological examination and the reverse transcriptasepolymerase chain reaction were performed to determine systolic blood pressure (SBP), collagen volume fraction (CVF), perivascular circumferential area (PVCA) of the heart and mRNA expression of cardiac chymase, typeI and typeIII collagen in chymase inhibitor (ChyI) group, spontaneously hypertensive rats (SHR) group and WistarKyoto (WKY) group. RESULTS The cardiac CVF, PVCA were (27±9)% and 0.4± 0.1 respectively in ChyI group vs (46±8)% and 1.9±0.9 in SHR group and (24±11)% and 0.4±0.1 in WKY group. Cardiac chymase in ChyI group decreased significantly compared with SHR group (P＜0.01, respectively), whereas there was no significant difference between ChyI group and WKY group. mRNA expressions of typeI and typeIII collagen and chymase in myocardial tissue in ChyI group were markedly lower than those in SHR group (P＜0.01, respectively), but as much as those in WKY group. No changes were observed in systolic blood pressure after ChyI was employed. CONCLUSION The cardiac chymase may be involved in the formation and degradation of extracellular matrix in myocardial tissue and may increase cardiac myofibrosis in spontaneously hypertensive rats.

参考文献/References

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糜酶抑制剂对自发性高血压大鼠心肌纤维化的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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