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Title:: Effects of Tetrandrine on phenotypic modulation of vascular smooth muscle cells and expression of mitogenactivated protein kinase phosphotase-1 after intimal injury of rabbit carotid arteries

作者:: 张新平¹; 庞月华²; 冯义伯¹; 付作林¹; 史春志¹; 谷翔¹; 1华中科技大学同济医学院附属协和医院心内科，湖北武汉 430022; 2新疆乌鲁木齐市友谊医院，新疆乌鲁木齐 830049

Author(s):: ZHANG Xin-ping¹; PANG Yue-hua²; FENG Yi-bo¹; FU Zuo-lin¹; SHI Chun-zhi¹; GU Xiang¹; 1.Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China; 2.Friendship Hospital, Urumchi, Xinjiang 830049, China

关键词:: 粉防己碱; 增殖细胞核抗原; 平滑肌α肌动蛋白; 丝裂原激活蛋白激酶磷酸酶1; 平滑肌细胞; 血管; 表型转化

Keywords:: Tetrandrine; Proliferation cell nuclear antigen; Smooth muscle α-actin; Mitogenactivated protein kinase phosphotase-1; Vascular smooth muscle cells; Phenotypic modulation

分类号:: R541.9

DOI:: -

文献标识码:: A

摘要:: 目的研究粉防己碱(tetrandrine,Tet)在防治血管内膜损伤后再狭窄(RS)与血管平滑肌细胞(VSMC)表型转化及丝裂原激活蛋白激酶磷酸酶-1(MKP-1)表达之间的关系。方法采用HE染色检测假损伤组(S组)、损伤组(I组)和损伤+Tet治疗组(Tet组) 28 d血管形态学改变;分别使用免疫组化和RT-PCR技术检测I组和Tet组7、14、28 d增殖细胞核抗原(PCNA)、平滑肌α肌动蛋白(SMα-actin)和MKP-1表达的变化。结果 ①28 d血管形态观察,S组血管壁各层结构完整; I组新生内膜(neointima,NI)面积显著增加,管腔面积(LA)显著缩小;Tet组NI增殖较I组明显减轻, LA增加。②损伤后7 d, Tet组与I组之间血管壁SMα-actin、PCNA和MKP-1表达变化无显著差异,NI增殖程度亦基本相同。Tet组14 d和28 d血管壁中PCNA表达均低于I组,而MKP-1表达均高于I组;14 d SMα-actin表达略高于I组,28 d两组间无差异。结论 Tet可不同程度地拮抗内膜损伤后VSMC表型转化及其调节,继而减缓NI增殖。

Abstract:: AIM To investigate the relationship between tetrandrine (Tet) on the prevention and treatment of restenosis and phenotypic modulation of vascular smooth muscle cells (VSMC) as well as the expression of mitogen activated protein kinase phosphotase-1 (MKP-1) after vascular intimal injury. METHODS HE staining was used to analyze the vascular morphology changes at the 28th day in the shaminjured, injured and Tettreated groups. Immunohistochemistry and RTPCR were respectively used to detect the expression changes of smooth muscleαactin (SMα-actin) and proliferation cell nuclear antigen(PCNA) and MKP-1 in injured group and Tet group at 7th, 14th and 28th days after balloon injury. RESULTS ①Each layer structure in the vascular wall of shaminjured arteries was intact at 28th day. Neointimal area was obviously increased and lumen area notably decreased in the injured group at 28th day after injury. In the group treated by Tet, the intimal proliferation was notably weaker than that in the injured group and the lumen area notably increased at 28th day. ②Compared with those in Tet group, the expression of SMα-actin, PCNA and MKP-1 in vascular walls in the injured group were not significantly different and so was the neointimal proliferation at 7th day after injury. The expression of PCNA in Tet group was significantly lower than that in injured group in vascular walls at 14th and 28th days after injury. However, the expression of MKP-1 in Tet group was significantly higher than that in injured group in vascular walls at 14th and 28th days after injury. The expression of SMα-actin in Tet group was slightly higher than that in injured group at 14th days but no significant difference was found between the two groups at 28th day. CONCLUSION Tet reduces to some degree the neointimal hyperplasia by inhibiting the VSMC phenotypic modulation and its regulation.

参考文献/References

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粉防己碱对内膜损伤后平滑肌细胞表型转化和MKP-1表达的影响(PDF)

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