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|本期目录/Table of Contents|

猪主动脉瓣膜去细胞基质体外酶解分析(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第2期
页码:
128-130
栏目:
基础研究
出版日期:
2006-03-01

文章信息/Info

Title:
Degradation of acellular porcine aorta valve in vitro
作者:
刘洋;  刘维永;  顾春虎;  李悟;  张近宝;  谭红梅
第四军医大学西京医院心脏外科， 陕西 西安710032
Author(s):
LIU Yang; LIU Wei-yong; GU Chun-hu; LI Wu; ZHANG Jin-bao; TAN Hong-mei
Department of Cardiovascular Surgery,Xijing Hospital,Fourth Military Medical University,Xi′an,Shaanxi 710032,China
关键词:
体外降解;  去细胞;  瓣膜;  细胞外基质
Keywords:
degradation in vitro; acellularise; valve;  ECM
分类号:
R654.2
DOI:
-
文献标识码:
A
摘要:
目的 研究猪主动脉瓣膜去细胞基质的体外降解过程。方法 应用胶原酶Ⅰ、弹性蛋白酶体外降解猪主动脉瓣膜去细胞基质，于不同时段观察降解失重率、降解液外观、蛋白及羟脯氨酸含量。结果 胶原酶和弹性蛋白酶对猪主动脉瓣膜去细胞基质均有较强的降解作用，胶原酶的作用较弹性蛋白酶强。结论 分析了猪主动脉瓣膜去细胞基质体外降解规律，为其可控性降解研究做了一定准备工作。
Abstract:
AIM To study the degradation behavior of acellular porcine aorta valve in vitro. METHODS Acellular porcine aorta valve was degaradated with collagenase I and elastase. The weight loss, value of protein and hydroxyproline were observed at different time points. RESULTS Both collagenase I and elastase markedly degradated the acellular porcine aorta valve in vitro, and collagenase I had a stronger degradation effect than elastase. CONCLUSION We have gained a better understanding of the degradation behavior of acellular porcine aorta valve in vitro, which will be helpful to the researches of degradation control.

参考文献/References

［1］O′Brien MF, Goldstein S, Walsh S, et al. The SynerGraft valve: a new acellular (nonglutaraldehydefixed) tissue heart valve for autologous recellularization first experimental studies before clinical implantation［J］. Semin Thorac Cardiovasc Surg,1999,11(4 Suppl 1):194-200.

［2］ 顾春虎，刘维永，张近宝,等. 去细胞猪主动脉瓣膜基质制备［J］. 中华实验外科杂志,2004,12（9）: 687-688.

［3］ Gratzera B, Santerrec JP, Leea JM, et al. Modulation of collagen proteolysis by chemical modification of amino acid sidechains in acellularized arteries［J］. Biomaterials, 2004, 25:2081-2094.

［4］Vaz CM,Yilmaz G,de Graaf LA,et al. In vitro degradation behaviour of biodegradable soy plastics: effects of crosslinking with glyoxal and thermal treatment l［J］. Polym Degrad Stab, 2003,81:65-74.

［5］ SchenkeLayland O,Vasilevski F,Opitz. K，et al. Impact of decellularization of xenogeneic tissue on extracellular matrix integrity for tissue engineering of heart valves［J］. J Struct Biol,2003, 143: 201-208.

［6］ Simionescu DT, Lovekamp JJ, Vyavahare NR. Extracellular matrix degrading enzymes are active in porcine stentless aortic bioprosthetic heart valves［J］. J Biomed Mater Res A， 2003,66(A): 755-763.

备注/Memo

备注/Memo:
收稿日期：2005-09-19.基金项目：国家自然科学基金项目资助（No.30271292） 通讯作者：刘维永，主任医师，教授，主要从事胸部战创伤组织工程瓣膜等方面的研究 Tel:(029)84775314 Email:weiyongl@fmmu.edu.cn 作者简介：刘洋，硕士生 Tel：（029）84775311 Email：liuyangl@fmmu.edu.cn
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