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|本期目录/Table of Contents|

辛伐他汀对NF-κBDNA结合活性和表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第4期
页码:
369-372
栏目:
基础研究
出版日期:
2006-08-25

文章信息/Info

Title:
Effects of simvastatin on NF-kappaB-DNA binding activity and expression in atherosclerotic plaque in rabbits
作者:
杨晓云; 周宁; 王琳; 曾和松; 卜军
华中科技大学同济医学院附属同济医院心内科，湖北 武汉 430030
Author(s):
YANG Xiao-yun;  ZHOU Ning;  WANG Lin;  ZENG He-song;  BU Jun
Department of Cardiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China
关键词:
辛伐他汀; 核因子κB; 动脉粥样硬化
Keywords:
simvastatin; nuclear factorkappaB; atherosclerosis
分类号:
R972;R541.4
DOI:
-
文献标识码:
A
摘要:
目的 观察辛伐他汀对兔动脉粥样硬化（AS）斑块中核因子-κB（NF-κB）-DNA结合活性及其表达的影响，进一步探讨辛伐他汀抗AS的作用机制。方法 将36只雄性新西兰大耳白兔随机分为正常对照组（NC）、高脂对照组（HC）和辛伐他汀组（HC+S）。实验中动态观察血清总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇（LDLC）的变化；实验结束时，用电泳移动迁移技术（EMSA）检测3组兔主动脉组织中NF-κB-DNA结合活性；用免疫组化技术观察各组血管组织中NF-κB的表达；显微镜下测定各组主动脉内膜厚度与斑块面积。结果 用药后4、8、12周时，（HC+S）组的TC、TG、LDL-C水平显著低于HC组（P<0.05），但高于NC组（P<0.05）；（HC+S）组的NF-κB-DNA结合活性及其表达强于NC组（P<0.05），但弱于HC组（P<0.05）；（HC+S）组的AS斑块面积及血管内膜厚度均大于NC组（P<0.05），但小于HC组（P<0.05）。结论 辛伐他汀可以抑制NF-κB-DNA结合活性及其表达，减轻AS的形成。
Abstract:
AIM To observe the effects of simvastatin on nuclear factorkappaB (NF-κB)-DNA binding activity and expression in atherosclerotic plaque of rabbits and to explore the mechanism of simvastatin in reducing atherosclerosis. METHODS Thirtysix male New Zealand white rabbits were randomly divided into normalCholesterol control group (NC), highCholesterol control group (HC) and highCholesterol+simvastatin group （HC+S）. The levels of serum total cholesterol (TC), triglyceride (TG) and Low density lipoprotein cholesteerol (LDL-C) were examined throughout the experiment. The NF-κB-DNA binding activity was measured with electrophoretic mobility shift assay (EMSA) and the expression of NF-κB in the rabbit aorta was detected with immunohistochemistry staining. The aortic plaque acreage and intimal thickness of aorta were measured with microscopy. RESULTS At the end of 4, 8 and 12 weeks, the levels of TC, TG and LDL-C in the （HC+S） group were significantly higher than those in the NC group, but significantly lower than those in the HC group（P<0.05）. At the end of experiment, the NFκBDNA binding activity and the expression of NF-κB in the （HC+S） group were significantly higher than those in the NC group, but significantly lower than those in the HC group（P<0.05）.The aortic plaque acreage and intimal thickness in the （HC+S） group were significantly higher than those in the NC group, but significantly lower than those in the HC group（P<0.05）. CONCLUSION This study demonstrates that simvastatin inhibits NF-κB-DNA binding activity, reduces the expression of NFκB protein and decreases atherosclerosis.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-07-01.基金项目：国家自然科学基金项目资助（No.30470713） 通讯作者：王琳，主任医师，主要从事心脏电生理研究 Tel:(027)83663607 Email:wanglin004900@yahoo.com.cn 作者简介：杨晓云，主治医师，博士生 Tel：(027)83663391 Email:yanglxiaoyun321@yahoo.com.cn
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