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反义MAPK寡核苷酸可抑制血管升压素诱导的心肌成纤维细胞一氧化氮合酶活性(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第4期
页码:
379-381
栏目:
基础研究
出版日期:
2006-08-25

文章信息/Info

Title:
Inhibitory effects of antisense MAPK oligodeoxynucleotide on NOS activity in cardiac fibroblasts induced by arginine vasopressin
作者:
范延红1; 赵连友2; 王海昌1
第四军医大学：1.西京医院心脏内科，2.唐都医院心脏内科，陕西 西安 710032
Author(s):
FAN Yan-hong1;  ZHAO Lian-you2;  WANG Hai-chang1
1.Department of Cardiology, Xijing Hospital, 2.Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi′an，Shaanxi 710032, China
关键词:
心肌成纤维细胞; 血管升压素; 一氧化氮合酶; 丝裂素活化蛋白激酶; 反义寡核苷酸
Keywords:
cardiac fibroblasts;  vasopressin;  nitric oxide synthase;  antisense mitogenactivated protein kinase;  antisense oligodeoxynucleotide
分类号:
R329.2;R331.3
DOI:
-
文献标识码:
A
摘要:
目的 探讨反义丝裂素活化蛋白激酶(MAPK)寡核苷酸对血管升压素(VP)诱导的心肌成纤维细胞(CFs)一氧化氮合酶(NOS)活性的抑制作用。方法 分离培养SD仔鼠CFs，采用分光光度法和RT-PCR测定CFs NOS活性和诱导型一氧化氮合酶(iNOS) mRNA表达。结果 VP显著提高CFs NOS活性和iNOS mRNA表达；反义MAPK 寡核苷酸剂量依赖性地抑制VP诱导下CFs NOS活性增高和iNOS mRNA表达增强，其中0.2 μmol/L 和0.3 μmol/L反义MAPK寡核苷酸可将VP诱导下CFs NOS活性和iNOS mRNA表达抑制到与对照组近似水平。结论 MAPK参与了VP诱导下CFs NOS活性增高和iNOS mRNA表达增强。
Abstract:
AIM To study the inhibitory effects of antisense mitogenactivated protein kinase (MAPK) oligodeoxynucleotide on nitric oxide synthase (NOS) activity in cardiac fibroblasts (CFs) induced by vasopressin (VP). METHODS CFs were isolated and cultured, and spectrophotometry and RTPCR were used to detect the nitric oxide synthase (NOS) activity and inducible nitric oxide synthase (iNOS) mRNA expression. RESULTS VP significantly enhanced the NOS activity and the iNOS mRNA expression in CFs. Antisense MAPK oligodeoxynucleotide inhibited the VPinduced enhancement of NOS activity and iNOS mRNA expression in a concentrationdependent manner. 0.2 μmol/L and 0.3 μmol/L antisense MAPK oligodeoxynucleotide decreased the NOS activity and iNOS mRNA expression to the levels of control. CONCLUSION MAPK is involved in the NOS activity increase in VPinduced CFs.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-06-01.通讯作者：赵连友，主任医师，主要从事高血压发病机制研究 Tel：（029）84777656 作者简介：范延红，主治医师，博士 Tel:(029)84775189 Email：fanyh1@fmmu.edu.cn
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