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MMP-2表达与大鼠动脉粥样硬化和血管钙化的关系及辛伐他汀对其影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第4期
页码:
376-378
栏目:
基础研究
出版日期:
2006-08-25

文章信息/Info

Title:
Relation between expression of MMP-2 and artery calcification in rats, and the effect of Simvastatin
作者:
卢维晟; 王一尘
上海第二医科大学附属新华医院老年科，上海 200092
Author(s):
LU Weisheng; WANG Yi-chen
Department of Geriatics， Affiliated Xinhua Hospital, Shanghai Second Medical University,Shanghai 200092，China
关键词:
动脉硬化; 血管钙化; 辛伐他汀; 基质金属蛋白酶-2
Keywords:
atherosclerosis; artery calcification; simvastatin; MMP-2
分类号:
Q344;R972
DOI:
-
文献标识码:
A
摘要:
目的 观察基质金属蛋白酶-2（MMP-2）随动脉粥样硬化和血管钙化病理变化过程的量效变化，并探讨辛伐他汀对其影响。方法 健康雄性SD大鼠44只，体质量150~200 g。随机分为5组：①对照组；②VitD3组；③ 高脂组；④ 高脂高钙组；⑤ 辛伐他汀组。应用免疫组化技术，对动脉粥样硬化大鼠主动脉MMP-2的表达进行测定。结果 高脂高钙组的MMP-2表达高于高脂组（P<0.05）。辛伐他汀组的MMP-2表达低于高脂高钙组（P<0.05）。辛伐他汀能抑制主动脉MMP-2的表达，并且随着MMP-2表达程度的降低，主动脉钙化程度也有所改善。结论 MMP-2表达与钙代谢及血管钙化密切相关。辛伐他汀减轻主动脉钙化的作用可能是通过影响MMP-2表达水平而实现的。
Abstract:
AIM To observe the changes of MMP-2 level during atherosclerosis and artery calcification, and to investigate the mechanism of simvastatin affecting the vessel calcium metabolism. METHODS Fortyfour male SD white rats, with body weight of 150-200 g, were divided into five groups at random: ① baseline group(n=8):normal diet; ② VitD3 group(n=9):normal diet+VitD3; ③ highcholesterol diet group(n=9):high cholesterol diet; ④ highcholesterolcalcium diet group (n=9):high cholesterol and high calcium diet; ⑤Simvastatin group(n=9):high cholesterol and high calcium diet, with simvastatin 10 mg/（kg·d) added. The content of MMP-2 in artery walls was determined by immunohistochemistry method. RESULTS The highcholesterolcalcium diet group, compared with highcholesterol diet group, had higher expression of MMP-2 (P<0.05). While compared with highcholesterolcalcium diet group, MMP-2 expression of the simvastatin group was lower (P<0.05). CONCLUSION MMP-2 expression is correlated with calcium metabolism and artery calcification. Simvastatin can restrain the expression of MMP2 in artery and therefore alleviate the artery calcification.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-01-20.作者简介：卢维晟，硕士 Tel：（021）65790000-7415 Email：weiyu19781@126.com
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